“…Test-tube dilution methods (with extract) provide easy approaches to a standard curve. FRICK (1964) specifically discussed methods of reducing variability in the results of glass slide spore germination assays of fungitoxicity and that discussion is useful for the bioassayist. This is a sensitive assay procedure, simple in its general outline but requiring careful controls of the organism source, culturing conditions, temperature, and other variables (NEELEY 1969).…”