Methods of paper chromatography of steroids applicable to the study of steroids in mammalian blood and tissues

Bush, I. E.

doi:10.1042/bj0500370

Cited by 1,083 publications

(192 citation statements)

References 13 publications

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“…In preliminary observations (44), it was found that, in the course of ACTH treatment of tissue cultures of rat adrenals, the enzyme systems of 18-and I l3hydroxylases were activated. This is in agreement with earlier reports concerning the enzymatic pathways of steroid biosynthesis in the rat adrenal cortex, in which it was shown that the adrenal cortex is capable of synthesizing corticosterone, 11 -deoxycorticosterone, 18-hydroxy-l I -deoxycorticosterone, and 18-hydroxycorticosterone (45)(46)(47)(48)(49)(50)(51)(52)(53)(54)(55)(56)(57)(58)(59)(60)(61) and that 1 7ahydroxylase is absent from the albino rat adrenal cortex (62)(63). Of the hydroxylases in steroid biosynthesis, 11/-and 18-hydroxylating enzymes were found in the mitochondrial fraction (64)(65)(66)(67)(68).…”

Section: Steroidogenic Activity Of the Cortical Mitochondriasupporting

confidence: 93%

Effects of Actinomycin D and Puromycin on the Acth-Induced Ultrastructural Transformation of Mitochondria of Cortical Cells of Rat Adrenals in Tissue Culture

Kahri

1968

The Journal of Cell Biology

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The ultrastructure of the mitochondria of the cultured cortical cells of rat adrenals was studied. In vivo it was found that the zona fasciculata mitochondria have vesicular internal structure. 600-A vesicles appear free in the matrix or as protrusions of the inner mitochondrial membrane. In tissue cultures of the fetal and newborn rat adrenal cortex it was seen that ACTH induces transformation of the tubulo-vesicular internal structure of the mitochondria to 600-A vesicles. Actinomycin D and puromycin inhibited this transformation if they were added with ACTH. When added alone, these inhibitors of protein synthesis induced no change in the ultrastructure of the mitochondria in cultured cortical cells of rat adrenals.

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Section: Steroidogenic Activity Of the Cortical Mitochondriasupporting

confidence: 93%

Effects of Actinomycin D and Puromycin on the Acth-Induced Ultrastructural Transformation of Mitochondria of Cortical Cells of Rat Adrenals in Tissue Culture

Kahri

1968

The Journal of Cell Biology

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“…The subjects in the present study were 33 pregnant females, 15 to (12). The chromatograms were studied as previously described (13).…”

Section: Methodsmentioning

confidence: 99%

Placental Passage of 17-Hydroxycorticosteroids: Comparison of the Levels in Maternal and Fetal Plasma and Effect of Acth and Hydrocortisone Administration 1

Migeon¹,

Prystowsky²,

Grumbach³

et al. 1956

J. Clin. Invest.

130

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A progressive rise in the levels of free plasma 17-hydroxycorticosteroids (17-OH-CS) during the course of pregnancy has been observed by a number of investigators (1-4). These findings are in conformity with the increase in urinary glucocorticoids previously reported (5). Normal adult concentrations have been found in the umbilical vein plasma obtained from the newborn infant at the time of delivery (2, 6-8), but significantly lower levels were observed in infants born by cesarean section (7).The site of production and the nature of plasma 17-OH-CS found in increased amounts during pregnancy and at delivery has not yet been elucidated. The maternal and fetal adrenals, as well as the placenta could be the source of C21 steroids with an a-ketolic side chain. A rise in plasma and urinary corticosteroids has been reported in pregnant Addisonian patients (9, 10) which has suggested that during pregnancy an extra-adrenal source, namely the placenta, may be secreting such hormones. The prompt decrease in plasma 17-OH-CS in the newborn infant during the first few days of life (6), and the very low umbilical cord plasma concentrations in infants delivered by cesarean section (7) could be interpreted as indicating a lack of fetal production of corticosteroids.The rather constant ratio of 17-OH-CS in the maternal as compared to the umbilical cord plasma in cases of vaginal delivery has suggested that

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“…50 filter paper for chromatography which had been washed with 2 N sodium hydroxide and distilled .water as described by Sharman (1963). A descending chromatogram was run using a modification of the Bush solvent system C (Bush, 1952). The solvent system was toluene: methanol: water: ethyl acetate in the proportions 10: 5 : 5 : 2 and the chromatogram was developed for 2.5 hr at 27°C after equilitbrating overnight.…”

Section: Resultsmentioning

confidence: 99%

Glycol metabolites of noradrenaline in brain tissue

Sharman

1969

British J Pharmacology

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A gas chromatographic method using electron capture detection is described for the estimation of those catecholamines and their metabolites which possess one or two free alcoholic hydroxyl groups. It is based on acetylation of phenolic hydroxyl and primary and secondary amino groups in aqueous solution, extraction into a water immiscible solvent and esterification of alcoholic hydroxyl groups with heptafluorobutyric anhydride. The method has been successfully applied to the detection and estimation of free 4‐hydroxy‐3‐methoxyphenylethylene glycol (MOPEG) and free 3,4‐dihydroxyphenylethylene glycol (DOPEG) in hypothalamic brain tissue. Although it was possible to measure noradrenaline and normetanephrine by the same method in pure solution, crude tissue extracts could not be used, despite their suitability for estimating the glycol metabolites.

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Methods of paper chromatography of steroids applicable to the study of steroids in mammalian blood and tissues

Cited by 1,083 publications

References 13 publications

Effects of Actinomycin D and Puromycin on the Acth-Induced Ultrastructural Transformation of Mitochondria of Cortical Cells of Rat Adrenals in Tissue Culture

Effects of Actinomycin D and Puromycin on the Acth-Induced Ultrastructural Transformation of Mitochondria of Cortical Cells of Rat Adrenals in Tissue Culture

Placental Passage of 17-Hydroxycorticosteroids: Comparison of the Levels in Maternal and Fetal Plasma and Effect of Acth and Hydrocortisone Administration 1

Glycol metabolites of noradrenaline in brain tissue

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