Effects of Actinomycin D and Puromycin on the Acth-Induced Ultrastructural Transformation of Mitochondria of Cortical Cells of Rat Adrenals in Tissue Culture

Kahri, Arvi

doi:10.1083/jcb.36.1.181

Cited by 70 publications

(17 citation statements)

References 52 publications

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“…Abrupt withdrawal of the drug or administration of levallorphan reproduced the change in mitochondrial structure which appeared in case of a single injection of codeine, and corresponded to the fall in body wt.. this resembled that in Actinomycin D (1,6) or Amphenone B (7) treated rats and re administration of codeine to withdrawn rats resulted in a return to the pretreated structure. A satisfactory explanation for the differences between morphine and codeine in mor phological changes of intramitochondrial structure, however, cannot be given only from the results of this experiment.…”

Section: Discussionmentioning

confidence: 78%

Studies on the Physical Dependence Liability of Analgesics

Yano¹,

Masuda²,

Nishino³

et al. 1973

Jpn.J.Pharmacol

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Abstract-Influence of codeine on the fine structure of mitochondria in the cells of zona fasciculata of rat adrenal cortex was studied electronmicroscopically. By the administration of codeine in a dose of 40 mg/kg, the number of intramitochondrial vesicles was decreased, the change being diminished during repeated administration of the drug. Withdrawal of codeine reproduced an extensive transformation, while with a re-injection of the drug, a prompt restoration to the pretreated structure was observed. The feasibility of utilizing this transformation as an index of physical dependence liability has been discussed.It has been evidenced that abrupt withdrawal of morphine or levallorphan challeng ing produces a marked transformation of ultrastructure of zona fasciculata mitochondria in adrenal cortex of the morphine addicted rats (1) and the time course of appearance of the transformation is in close agreement with that of loss in body wt. after withdrawal, this being the best index of physical dependence liability. On the other hand, CG-315, a newly synthesized analgesic, shows neither the mitochondrial transformation nor fall in body wt. after withdrawal of the drug during chronic administration (2, 3).The present experiments were undertaken to clarify whether codeine with moderate physical dependence liability (4, 5) is able to produce the mitochondrial transformation after withdrawal.MATERIALS AND METHODS Unless otherwise noted, the experimental rat strain, feeding condition and procedures for electron-microscopic examination were the same as described in the first report (1).In chronically administered experiments, rats were given four s.c. injections of codeine daily 6 hr intervals (6 a.m., noon, 6 p.m. and midnight) for 50 days. The initial dose of the drug was 20 mg/ kg/day during the first week (5 mg/kg x 4 daily), 40 mg/kg in the second week, 60 mg/kg in the third week, 80 mg/kg in the 4th week and 100 mg/kg in the 5th week until sacrifice.

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Section: Discussionmentioning

confidence: 78%

Studies on the Physical Dependence Liability of Analgesics

Yano¹,

Masuda²,

Nishino³

et al. 1973

Jpn.J.Pharmacol

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“…Later, Sabatini et al, (1962) reported that mitochondria of fasciculata cells in hypophysectomized rat were provided with the straight tubular cristae as similar to those of zona glomerulosa cells, and these cristae were transformed to the vesicular type after ACTH administration. The findings of Kahri (1968) that tubular cristae of cultured adrenocortical cells were reverted to vesicular type under the added ACTH in vitro. He indicated that the mitochondrial cristae is under the control of ACTH, and may be related to the conversion into the original cell function.…”

Section: Discussionmentioning

confidence: 97%

Studies on Regenerating Adrenal Cortex 2. Autoradiographic and Electron Microscopic Observations

Seki¹,

Sekiyama²,

Miyahara³

et al. 1969

Endocrinol Japon

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“…A tissue culture method suitable for long-term cultivation [Kauri, 1966] was used. The medium consisted of 50% Melnick's solution A (Hanks' balanced salt solution -[-0.5 % lactalbumin hydrolysate); 25% calf serum (ultrafiltrated 4-heat inactivated 30 min at 56 °C); 25 % amino acid Parker (Pharmaceutical Manufactures, Orion Oy, Finland).…”

Section: Methodsmentioning

confidence: 99%

“…Several in vitro studies [Kauri, 1966[Kauri, , 1968b have shown that in the rat adrenal cortex, ACTH plays the main role in the differentiation of zona fasciculata and reticularis cells from poorly differentiated zona glomerulosa -zona inter media cells. Several hypotheses have been offered to explain the differences in functional activity of the fetal zone cells of the human embryo and zona fasciculata of the adult cortex, and even more speculative opinions exist on the mechanism which controls the functional activity and morphological differentiation of the fetal zone cells of the adrenal cortex in humans.…”

Section: Introductionmentioning

confidence: 99%