Methods of detection of <i>Chlamydia psittaci</i> in domesticated and wild birds

McElnea, Catriona; Cross, G. M.

doi:10.1111/j.1751-0813.1999.tb12123.x

Cited by 35 publications

(21 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Olsen et al (1998) used PCR to test fecal samples and found 9/312 (2.89%) positive birds in Sweden. McElnea and Cross (1999) also used PCR as one of the methods for C. psittaci detection in domestic and wild birds and observed 16% positive samples among captured birds with no clinical signs of illness, similarly to the present study (16.8%). These findings assure the importance of birds, especially pigeons, as C. psittaci-carriers in Brazil.…”

Section: Discussionsupporting

confidence: 71%

Chlamydophila psittaci and Toxoplasma gondii infection in pigeons (Columba livia) from São Paulo State, Brazil

Lima

Langoni

Silva

et al. 2011

Veterinary Parasitology

View full text Add to dashboard Cite

Pigeons (Columba livia) cohabit with humans in urban and rural areas, representing a public health problem since microorganisms are transmitted through the inhalation of dust from their dry feces (chlamydiosis) and through ingestion of their undercooked or poorly refrigerated meat (toxoplasmosis). This study aimed to evaluate the presence of Chlamydophila psittaci and Toxoplasma gondii in pigeons from four cities in São Paulo State, Brazil. C. psittaci was evaluated through hemi-nested polymerase chain reaction (hnPCR) using cloacal and tracheal swabs, whereas T. gondii specific antibodies were assessed by means of modified agglutination test (MAT), mouse brain and muscle bioassay, and polymerase chain reaction (PCR). To confirm the infection in mice, T. gondii antibodies were assayed by using indirect fluorescent antibody test (IFAT). Considering C. psittaci, 40/238 (16.8%; 95%CI 12.6-22.1%) samples were positive according to hnPCR, especially for the cities of São Paulo (42.5%) and Bauru (35%). As regards T. gondii, 12/238 (5%; 95%CI 2.9-8.6%) serum samples were positive according to MAT. Of these, five samples had titer equal to 1:8; six samples, 1:16; and one sample, 1:32. Bioassay, IFAT and PCR were negative for mouse toxoplasmosis. The absence of T. gondii antibodies suggests that pigeons may be infected with a low concentration of the agent, not detected by the antigen test. Thus, C. psittaci represents an actual problem concerning bird health.

show abstract

Section: Discussionsupporting

confidence: 71%

Chlamydophila psittaci and Toxoplasma gondii infection in pigeons (Columba livia) from São Paulo State, Brazil

Lima

Langoni

Silva

et al. 2011

Veterinary Parasitology

View full text Add to dashboard Cite

show abstract

“…The major advantage of this study was the use of an internally controlled real-time PCR assay. PCR is a sensitive and specific test compared to enzymelinked immunosorbent assays and tissue culture assays available for C. psittaci detection in birds (7,9). Salinas et al reported one of the largest series on the prevalence of C. psittaci in feral pigeons (10).…”

mentioning

confidence: 99%

Prevalence of Chlamydophila psittaci in Fecal Droppings from Feral Pigeons in Amsterdam, The Netherlands

Heddema

Sluis

Buys

et al. 2006

Appl Environ Microbiol

View full text Add to dashboard Cite

show abstract

“…The vaginal swab samples were inoculated into Vero cells, obtained from the Department of Virology, Faculty of Veterinary Medicine, Cairo University, Egypt, and 6-day-old embryonated chicken eggs, as described previously (McElnea and Cross 1999;Celebi and Seyyal 2006;Anonymous 2008).…”

Section: Isolation In Vero Cell Culture and Embryonated Eggsmentioning

confidence: 99%

Chlamydiaceaein riverine buffalo (Bubalus bubalis) and cows (Bos taurus) in Egypt with and without signs of reproductive disease

Osman

Ali

El-Jakee

et al. 2012

New Zealand Veterinary Journal

View full text Add to dashboard Cite

Methods of detection of Chlamydia psittaci in domesticated and wild birds

Abstract: PCR-dot blot detection of C psittaci in birds appears to be more sensitive than cell culture isolation in this study. C psittaci infection of birds may occur in clinically normal captive birds.

Cited by 35 publications

References 38 publications

Chlamydophila psittaci and Toxoplasma gondii infection in pigeons (Columba livia) from São Paulo State, Brazil

Chlamydophila psittaci and Toxoplasma gondii infection in pigeons (Columba livia) from São Paulo State, Brazil

Prevalence of Chlamydophila psittaci in Fecal Droppings from Feral Pigeons in Amsterdam, The Netherlands

Chlamydiaceaein riverine buffalo (Bubalus bubalis) and cows (Bos taurus) in Egypt with and without signs of reproductive disease

Contact Info

Product

Resources

About