Methods of Analysis (Extraction, Separation, Identification and Quantification) of Carotenoids from Natural Products

Butnariu, Monica

doi:10.4172/2157-7625.1000193

Cited by 108 publications

(81 citation statements)

References 49 publications

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“…For the hot extraction are used several methods, i.e., the reflux extraction, solid-liquid or liquid-liquid extraction. The best results are obtained by the ultra-sonication of the material extracted with solvents -the ultrasound-assisted extraction (UAE) and the supercritical fluid extraction (SFE) [20][21].…”

Section: Introductionmentioning

confidence: 99%

“…Much effort has been devoted to developing HPLC-mass spectrometry (HPLC-MS) methods with mainly atmospheric pressure ionization interfaces (APCI) or electrospray ionization interfaces (ESI) [30]. Also, capillary electrophoresis (CE) [31], high 3 performance thin layer chromatography (HPTLC), ultraviolet-visible (UV-Vis), infrared (IR) and Raman spectrometry have been employed as an analytical technique to identify and quantify low levels and various forms of carotenoids [21,[32][33][34].…”

Section: Introductionmentioning

confidence: 99%

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Extraction and Analysis of the Major Carotenoids of Agro-Industrial Waste Materials Using Sequential Extraction Techniques and High Performance Liquid Chromatography

Rubashvılı¹,

Tsitsagi²,

Ebralidze³

et al. 2018

Eurasian J Anal Chem

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The objective of the present study was to develop sequential extraction procedures for the major carotenoids -beta-carotene and lycopene from agro-industrial waste materials -tomato skin, tangerine and orange peels using the ultrasound-assisted extraction and the supercritical fluid extraction techniques. A rapid, effective and selective high performance liquid chromatographic method for quantitative determination of beta-carotene and lycopene in organic extracts solutions was developed and validated with respect to robustness, specificity, linearity-range, accuracy, precision, limit of detection (LOD) and quantitation (LOQ) as well. The effect of the operating pressure, the temperature, the extraction time, the flow rate of supercritical fluid, the sample size, the ultrasound power and the solvent nature used was investigated. The optimal conditions for extraction were found. The LOD and the LOQ are 0.0081µg/mL and 0.00405 µg/mL for beta-carotene, 0.034 µg/mL and 0.0085 µg/mL for lycopene, respectively. No interference was observed. The content of betacarotene per 1 g of dried agro-industrial waste material varies 8.39 -12.75 µg (tomato skin), 25.65 -32.18 µg (tangerine peel), 41.66 -59.16 µg (orange peel) and the content of lycopene -165.11 -179.56 µg (tomato skin), 11.12 -17.91 µg (tangerine peel), 8.37 -10.65 µg (orange peel).

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Extraction and Analysis of the Major Carotenoids of Agro-Industrial Waste Materials Using Sequential Extraction Techniques and High Performance Liquid Chromatography

Rubashvılı¹,

Tsitsagi²,

Ebralidze³

et al. 2018

Eurasian J Anal Chem

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“…Following LC/MS of TLC-separated fractions, two derivatives namely tangeraxanthin and 4-ketonostoxanthin were identified. Tangeraxanthins are naturally occurring retro-apo carotenoids (Butnariu 2016). Another carotenoid (4-ketonostoxanthin 3′-sulphate), similar to 4-ketonostoxanthin, was also reported earlier by Yokoyama et al (1996) in a marine bacterium.…”

Section: Discussionmentioning

confidence: 99%

Optimisation and characterisation of the orange pigment produced by a cold adapted strain of Penicillium sp. (GBPI_P155) isolated from mountain ecosystem

et al. 2018

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With globalisation and growing health risks of synthetic colourants, search for pigments from natural sources has increased owing to their non-toxic nature. The present study highlights the bioprospection of natural pigment from a cold adapted fungal strain of Penicillium sp. (GBPI_P155), isolated from soil of Indian Himalayan region. The fungus produced insoluble and orange-coloured pigment in liquid medium with maximum production recorded in potato dextrose (PD) broth at 15°C and 3 pH, while maximum biomass was produced at 25°C and pH 3. While examining the effect of different mineral salts, and carbon and nitrogen sources on pigment production, maximum accumulation of pigment was recorded in PD broth supplemented with 2% maltose. Following production, extraction of pigment was performed using chloroform and characterised partially by UV/vis (λ max at 495 nm and a shoulder peak at 530 nm) and Fourier Transform Infrared (FT-IR) spectroscopy. Thin layer chromatography of chloroform extract resulted in separation of pigment in three fractions with Rf values 0.911, 0.852 and 0.808, which were further analysed using Liquid Chromatography Mass Spectrometry (LC/MS). The overall approach resulted in identification of pigment as a mixture of different derivatives of carotenoids. The extracted pigment also possessed antimicrobial activity against Gram-positive and Gram-negative bacteria and actinobacteria.

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“…(Devlin & Witham 1997). Both Chl-a and Chl-b pigments are associated with light harvesting processes (Butnariu, 2016), which are solely responsible for photosynthesis in higher plants. Chlorophyll concentration in leaves is an indicator of plant health.…”

Section: Research Articlementioning

confidence: 99%

Ethno-medicinal Species of Cassia (C. fistula, C. siamea, C. tora): Documentation and Metabolites Estimation

Rani

Munjani

Lakra

et al. 2019

Int J Appl Sci Biotechnol

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Different species of Cassia found in Jharkhand are consumed as daily vegetables have high nutritional value. Most of them are underutilized and display varied ethenomedicinal values. The work was carried out with an aim to document three ethno medicinal properties of three different species of Cassia, Cassia fistula, Cassia siamea and Cassia tora. The documentation was done among 100 tribal people residing in the area near Morabadi, Ranchi. The knowledge revealed that the three plants were used in almost every tribal houses as a dietary supplement and various medicinal uses. According to the Munda Vaidya’s the plant Cassia is of pitta nature and its different part have different uses. The root is used against tuberculosis glands, diabetes, as a tonic, the root and bark paste mixed in equal amount is used against snake bite. traditionally it is used as laxative, for the treatment of leprosy and various skin disorders. the present study aimed to screen and quantify primary and secondary metabolite by quantitative and qualitative method. The pigments (chlorophyll and carotenoid) were characterised by UV visible spectroscopy. Concentration of chlorophyll-a, chlorophyll-b and carotenoid was calculated by Arnon method (80% acetone extract). The spectrophotometric study of the different pigment of the three species of Cassia, Cassia fistula (chl-a 254.516, chl-b 305.741, total chl 533.7 carotenoid 10.6), Cassia siamea (chl-a 257.88, chl-b 393.16, total chl 630.2 carotenoid 9.395), Cassia tora (chl-a 266.36, chl-b 271.96, total chl 538.32 carotenoid 16.3). The result revealed that chl-a was highest in Cassia tora followed by Cassia siamea and Cassia fistula. Chl-b pigment followed C. siamea> C. fistula>C. tora pattern, total chl C. siemea>C. tora>C. fistula and the carotenoid amount C. tora > C. fistula > C. siamea. In screening of secondary metabolites tannin, saponins alkaloids, terpenoids, resin Phyto-sterols were carried out on the aqueous extract showed +ve test for all except phyto-sterols and on the powdered specimen gave –ve result in aqueous, ethanol as well as acetone extract. Int. J. Appl. Sci. Biotechnol. Vol 7(3): 387-394

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Methods of Analysis (Extraction, Separation, Identification and Quantification) of Carotenoids from Natural Products

Cited by 108 publications

References 49 publications

Extraction and Analysis of the Major Carotenoids of Agro-Industrial Waste Materials Using Sequential Extraction Techniques and High Performance Liquid Chromatography

Extraction and Analysis of the Major Carotenoids of Agro-Industrial Waste Materials Using Sequential Extraction Techniques and High Performance Liquid Chromatography

Optimisation and characterisation of the orange pigment produced by a cold adapted strain of Penicillium sp. (GBPI_P155) isolated from mountain ecosystem

Ethno-medicinal Species of Cassia (C. fistula, C. siamea, C. tora): Documentation and Metabolites Estimation

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