Methods in plant histology,

Chamberlain, Charles J.

doi:10.5962/bhl.title.163242

Cited by 81 publications

(33 citation statements)

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“…The cell wall and other cytoplasmic structures also stain in these sections; however, in fresh stigmas, tissue permeability is limited by cuticular development, hence, staining of these structures would not be expected until cuticle exfoliation. Cell walls, starch grains, cytoplasm, the spindle apparatus, mitochondria, and chromosomes, typically stain with crystal violet (Chamberlain, 1924;Clark, 1973). Cuticle changes may also be responsible for improved cytoplasmic fixation as papillae mature.…”

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confidence: 99%

Development of the Stigmatic Surface of Prunus Avium L., Sweet Cherry

Uwate

Lin

1981

American J of Botany

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The stigmatic papillae of sweet cherry were examined to determine developmental characteristics of the wet‐stigma surface. Early stages of secretion are detectable 1 wk prior to anthesis by using a 1% crystal violet solution. The number of stainable cells and the amount of interstitial staining subsequently increase, although secretions are not visible on unstained specimens until anthesis. Auto‐fluorescence above 500 nm (excited by 335–480 nm) becomes microscopically detectable at floral maturity and grows more intense after anther dehiscence. Light microscopy of plastic sections shows that papillae degenerate in peripheral regions of unpollinated mature stigmas, and that this is even more pronounced in pollinated ones. The distal portions of the papillae are covered with a homogeneous cuticular cap, which when viewed with electron microscopy encloses a subcutaneous secretion prior to cuticle exfoliation. Other exudates observed with electron microscopy prior to anthesis are interstitial electron‐translucent globules and surrounding matrix, and assorted vesicles, lipid globules, and starch grains which are present at floral maturity. Flowers observed under field conditions in the terminal secretion stage accumulate trichomatous structures. Our observations indicate that the stigma of Prunus avium L. is characterized by several phases of secretion which appear to be facilitated by mechanical abrasion. A model for the primary pollen‐receptive area is proposed and suggestions are made concerning the origin of the secretions.

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Development of the Stigmatic Surface of Prunus Avium L., Sweet Cherry

Uwate

Lin

1981

American J of Botany

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“…For obtaining radial sections of buds and adjusting areas of the stem the method of imbedding into paraffin proved to be useless due to a strong lignification of the woody parts of branches. Therefore we treated our material with acetylcellulose according to CHAMBERLAIN (1935) to soften the lignified sections of the Abutilon fixed nodes. The sections were made with the aid of freezing microtome and dyed with safranin.…”

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confidence: 99%

On the Mechanism of the Liberation of Abutilon Thompsonii from the Virus of “Infectious Chlorosis” under the Influence of Defoliation

Terekhova

1964

Journal of Phytopathology

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“…For the histological observation, heart-shaped somatic embryos were fixed in formalin-acetic acid-alcohol, dehydrated in a tertiary-butanol series, and embedded in paraplast. Serial sections were cut at 8 µm and stained with Delafield's Hematoxylin (Chamberlain, 1924), Callus arose from the peripheral region of zygotic embryo cotyledons on all media containing 2,4-D and kinetin after 4 weeks of culture. After 6 weeks, callus from the entire surface of the zygotic embryos, including cotyledons, hypocotyls, and radicles, gave rise to somatic embryos.…”

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In Vitro Flowering of Plantlets Regenerated from Zygotic Embryo-derived Somatic Embryos of Ginseng

Lee¹,

Liu²,

Yang³

et al. 1990

HortSci

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Mature zygotic embryos dissected from ginseng (Panax ginseng C.A. Meyer) seeds were cultured on Murashige and Skoog (MS) medium containing various concentrations of 2,4-D and kinetin. Somatic embryos were induced directly from cotyledonary tissue and from intervening callus. The frequency of somatic embryo induction was up to 55% of zygotic embryo explants. Upon transfer onto half-strength MS medium supplemented with 1 mg BA/liter and 1 mg GA3/liter, most somatic embryos developed into plantlets. More than 50% of the plantlets flowered after 4 weeks of culture, and some developed immature fruits in vitro. These results indicate that adulthood of ginseng root explants is not a prerequisite for flowering of plantlets regenerated through somatic embryogenesis. Chemical names used: (2,4 -dichlorophenoxy) acetic acid (2,4-D); N-(2-furanylmethyl) -1H-purin-6-amine(kinetin); N-(phenylmethyl) -1H-purin-6-amine (BA); gibberellic acid (GA3).

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Methods in plant histology,

Cited by 81 publications

References 0 publications

Development of the Stigmatic Surface of Prunus Avium L., Sweet Cherry

Development of the Stigmatic Surface of Prunus Avium L., Sweet Cherry

On the Mechanism of the Liberation of Abutilon Thompsonii from the Virus of “Infectious Chlorosis” under the Influence of Defoliation

In Vitro Flowering of Plantlets Regenerated from Zygotic Embryo-derived Somatic Embryos of Ginseng

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