Methods for the estimation of the number and quality of animal cells immobilized in carbohydrate gels

Al-Rubeai, M.; Musgrave, S.C.; Lambe, C. A.; Walker, A.G.; Evans, Natasha; Spier, R.E.

doi:10.1016/0141-0229(90)90058-x

Cited by 10 publications

(2 citation statements)

References 4 publications

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“…It was not possible to use the FA labelling technique directly on activated sludge flocs obtained from waste water (17). A qualitative spatial distribution of one artificially immobilized species has been determined with an FA labelling technique (1,3,11,13,14,24).…”

Section: Tionsmentioning

confidence: 99%

Quantitative Determination of the Spatial Distribution of Nitrosomonas europaea and Nitrobacter agilis Cells Immobilized in κ-Carrageenan Gel Beads by a Specific Fluorescent-Antibody Labelling Technique

Hunik

Hoogen

Boer

et al. 1993

Appl Environ Microbiol

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A novel technique, combining labelling and stereological methods, for the determination of spatial distribution of two microorganisms in a biofilm is presented. Cells ofNitrosomonas europaea (ATCC 19718) and Nitrobacter agilis (ATCC 14123) were homogeneously distributed in a k-carrageenan gel during immobilization and allowed to grow out to colonies. The gel beads were sliced in thin cross sections after fixation and embedding. A two-step labelling method resulted in green fluorescent colonies of either N. europaea or N. agilis in the respective cross sections. The positions and surface areas of the colonies of each species were determined, and from that a biomass volume distribution for N. europaea and N. agilis in K-carrageenan gel beads was estimated. This technique will be useful for the validation of biofilm models, which predict such biomass distributions.

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Section: Tionsmentioning

confidence: 99%

Quantitative Determination of the Spatial Distribution of Nitrosomonas europaea and Nitrobacter agilis Cells Immobilized in κ-Carrageenan Gel Beads by a Specific Fluorescent-Antibody Labelling Technique

Hunik

Hoogen

Boer

et al. 1993

Appl Environ Microbiol

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“…Several reports on the application of MTT as a calorimetric assay for the qualitative (Ohlson et al, 1994) and quantitative analysis of cells in porous carriers or agarose gel (Yamaji et al, 1992, Al-Rubeai et al, 1990 have been described. In these papers, formazan formed from MTT by the myeloma or hybridoma was solubilized with isopropanol or by sonication.…”

Section: Introductionmentioning

confidence: 99%

Improved MTT(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assay for the measurement of viable animal cell number in porous cellulose carriers

et al. 1996

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A method to measure cell concentration in porous cellulose carriers was developed. Results of the comparison of 5 calorimetric assays showed that MlT(3-(4,5-dimethylthiazol-2-yl)-2.5diphenyl tetrazolium bromide) assay by the solubilization of the formazan with DMSO(dimethy1 sulfoxide) was the most suitable rnetbod for the measurement of cell concentration in porous cellulose carriers. The growth of CHO-Kl (Chinese hamster ovary)-immobilized in different types of carrier was evaluated.

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Production of Biologics from Animal Cell Cultures

Warnock

Al‐Rubeai

2005

Applications of Cell Immobilisation Biotechnology

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Methods for the estimation of the number and quality of animal cells immobilized in carbohydrate gels

Cited by 10 publications

References 4 publications

Quantitative Determination of the Spatial Distribution of Nitrosomonas europaea and Nitrobacter agilis Cells Immobilized in κ-Carrageenan Gel Beads by a Specific Fluorescent-Antibody Labelling Technique

Quantitative Determination of the Spatial Distribution of Nitrosomonas europaea and Nitrobacter agilis Cells Immobilized in κ-Carrageenan Gel Beads by a Specific Fluorescent-Antibody Labelling Technique

Improved MTT(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assay for the measurement of viable animal cell number in porous cellulose carriers

Production of Biologics from Animal Cell Cultures

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