Methods for monitoring and imaging nanoparticles in cells

Lapotko, Dmitri O.; Lukianova, Ekaterina; Чижик, С. А.

doi:10.1117/12.712855

Cited by 5 publications

(5 citation statements)

References 26 publications

(41 reference statements)

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“…We have found that PTB lifetime almost linearly increased with the increase of pump laser fluence (both for NPs and their clusters) and thus the maximal diameter of PTBs can be controlled though the level of laser pulse fluence (Figure 5A). Additional details can be found in [29,51,55–58]. …”

Section: Plasmonic Np-generated Ptbsmentioning

confidence: 99%

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Plasmonic Nanoparticle-Generated Photothermal Bubbles and Their Biomedical Applications

2009

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This article is focused on the optical generation and detection of photothermal vapor bubbles around plasmonic nanoparticles. We report physical properties of such plasmonic nanobubbles and their biomedical applications as cellular probes. Our experimental studies of gold nanoparticle-generated photothermal bubbles demonstrated the selectivity of photothermal bubble generation, amplification of optical scattering and thermal insulation effect, all realized at the nanoscale. The generation and imaging of photothermal bubbles in living cells (leukemia and carcinoma culture and primary cancerous cells), and tissues (atherosclerotic plaque and solid tumor in animal) demonstrated a noninvasive highly sensitive imaging of target cells by small photothermal bubbles and a selective mechanical, nonthermal damage to the individual target cells by bigger photothermal bubbles due to a rapid disruption of cellular membranes. The analysis of the plasmonic nanobubbles suggests them as theranostic probes, which can be tuned and optically guided at cell level from diagnosis to delivery and therapy during one fast process. Keywordscell; imaging; laser; plasmonic nanoparticle; photothermal; theranostics; vapor bubble Biomedical applications of plasmonic nanoparticles (NPs) have demonstrated their biocompatibility [1,2], excellent optical scattering [3][4][5][6][7] and photothermal (PT) [8][9][10][11][12] properties and high photo-and thermal stability in comparison to any molecular optical absorbers. The combination of PT-sensing techniques [13] with plasmonic properties of the NPs has shown very promising results with a detection limit of several nanometers [14]. However, the sensitivity of PT sensing requires an increase in the laser-induced temperature, which may cause thermal damage to cells and tissues. Laser-induced PT phenomena include the initial thermalization of NPs that, in turn, rapidly causes several environmental thermal processes: the heating of the surrounding media [15][16][17] (due to thermal diffusion), its vaporization (if the temperature exceeds the vaporization threshold) and the generation of acoustic and shock waves [18]. Pulses that are too long (or continuous optical activation) cause a large spatial spread of the thermal field (many orders of magnitude larger than NP size) due to the thermal diffusion. This limits the selectivity and safety of NP-based PT diagnostics and therapy. Ultra-short laser pulses concentrate the thermal field within the NP but generate pressure (and shock) waves that also spread over a large volume and may cause uncontrollable damage [19]. The sensitivity, safety and specificity of NP methods are limited at cell and molecular levels by the strong scattering background of a highly heterogeneous bioenvironment and also by the incidental (nonspecific) accumulation of NPs in normal cells and tissues. Therefore, despite the apparent advantages of nanomaterials, their biomedical application does not yet bring a significant gain on the established methods. NIH Public AccessAutho...

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Section: Plasmonic Np-generated Ptbsmentioning

confidence: 99%

“…Formation of clusters does not compromise viability of the cells. Additional details can be found in [51,53,58,89,90]. …”

Section: Biomedical Properties Of Gold Np-generated Ptbsmentioning

confidence: 99%

Plasmonic Nanoparticle-Generated Photothermal Bubbles and Their Biomedical Applications

2009

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“…(1) synthesis of new biomarkers with minimum overlap of their emission spectra with the background fluorescence and (2) development of advanced experimental techniques permitting one to decrease the background signal. [26][27][28][29][30][31][32][33][34] Intense work is being carried out on the search for and synthesis of nanoparticles with optimal properties for biological applications as biomarkers, absorbents, and drug carriers. [2][3][4][5] The existing biomarkers have several drawbacks.…”

Section: Introductionmentioning

confidence: 99%

“…Most of the methods of optical imaging are based on optical microscopy. [26][27][28][29][30] Confocal laser scanning microscopy (CLSM) is a commonly used tool that provides morphological and functional information within cells and tissues. 28,29 It was shown that the unique capabilities of CLSM such as the ability to detect fluorescence, three-dimensional (3-D) image reconstruction, and use of the reflection mode in tandem with other methods, provide great promise with broad applications for the visualization of various nanoparticles such as quantum dots, fullerenes, dendrimers, for example, at their penetration into the surface of the skin.…”

Section: Introductionmentioning

confidence: 99%

Optical imaging of fluorescent carbon biomarkers using artificial neural networks

et al. 2014

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The principle possibility of extraction of fluorescence of nanoparticles in the presence of background autofluorescence of a biological environment using neural network algorithms is demonstrated. It is shown that the methods used allow detection of carbon nanoparticles fluorescence against the background of the autofluorescence of egg white with a sufficiently low concentration detection threshold (not more than 2 μg/ml for carbon dots 3 μg/ml and for nanodiamonds). It was also shown that the use of the input data compression can further improve the accuracy of solving the inverse problem by 1.5 times.

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“…Table provides a sampling of the most common microscopy techniques for the visualization specifically of inorganic nanoparticles, including their prominent advantages and disadvantages. Although innovative techniques involving confocal Raman mapping, − atomic force microscopy, − and magnetic force microscopy , have been used, TEM and fluorescence microscopy are by far the most common. In particular, TEM is valuable because of the extremely high resolution (<1 nm depending on experimental parameters) that allows for visualization of nanoparticle localization in cellular compartments as well as an accurate assessment of the nanoparticle state (aggregation, shape change, etc.).…”

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confidence: 99%

Dark Field Transmission Electron Microscopy as a Tool for Identifying Inorganic Nanoparticles in Biological Matrices

et al. 2015

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Dark field transmission electron microscopy has been applied herein to visualize the interactions of inorganic nanomaterials with biological systems. This new application of a known technique addresses a deficiency in status quo visualization techniques. High resolution and low noise images can be acquired to locate and identify crystalline nanoparticles in complex biological matrices. Moreover, through the composition of multiple images taken at different angular beam tilts, it is possible to image a majority of nanoparticles present at a site in dark field mode. This facilitates clarity regarding the internalization of nanomaterials in cellular systems. In addition, comparing dark field images recorded at different angular tilts yields insight into the character of nanoparticle faceting.

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Methods for monitoring and imaging nanoparticles in cells

Cited by 5 publications

References 26 publications

Plasmonic Nanoparticle-Generated Photothermal Bubbles and Their Biomedical Applications

Plasmonic Nanoparticle-Generated Photothermal Bubbles and Their Biomedical Applications

Optical imaging of fluorescent carbon biomarkers using artificial neural networks

Dark Field Transmission Electron Microscopy as a Tool for Identifying Inorganic Nanoparticles in Biological Matrices

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