Methods for Lipase Immobilization and Their Use for Biodiesel Production from Vegetable Oil

Yücel, Yasin; Demir, Cevdet; Dizge, Nadir; Keskinler, Buelent

doi:10.1080/15567036.2010.545805

Cited by 9 publications

(1 citation statement)

References 7 publications

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“…However, Lipozyme TL IM is essentially inactive after 3 batches of the methyl esterification reaction due to Lipozyme TL IM absorb water in the reaction and lead to self-cleavage. Compared with the immobilized enzymes reported by Yücel et al (2014), the catalytic activity and batch stability have been greatly improved. The CLEA-TLL@NKA had high stability and esterification activity, making it ideal biocatalyst for large-scale applications.…”

Section: Batch Stability Of Clea-tll@nkamentioning

confidence: 99%

Immobilization of Lipozyme TL 100L for methyl esterification of soybean oil deodorizer distillate

et al. 2020

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An immobilization method for binding cross-linked enzyme aggregates of Lipozyme TL 100L on macroporous resin NKA (CLEA-TLL@NKA) was developed in this study. The esterification activity of CLEA-TLL@NKA reached 6.4 U/mg. The surface structure of immobilized lipase was characterized by scanning electron microscopy. Methyl esterification reaction of soybean oil deodorizer distillate (SODD) was catalyzed by CLEA-TLL@NKA, which the conversion rate reached 98% and its activity retained over 90% after 20 batches of reaction. Compared with the commercial enzyme Lipozyme TLIM, half-life (t 1/2 ) of CLEA-TLL@NKA increased by 25 times and the catalytic activity increased by approximate 10 times. Thus, CLEA-TLL@NKA had high catalytic activity, good operational stability, and potential industrial application in the field of oil processing.

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Section: Batch Stability Of Clea-tll@nkamentioning

confidence: 99%

Immobilization of Lipozyme TL 100L for methyl esterification of soybean oil deodorizer distillate

et al. 2020

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Enhancement of biochemical aspects of lipase adsorbed on halloysite nanotubes and entrapped in a polyvinyl alcohol/alginate hydrogel: strategies to reuse the most stable lipase

Mohammadi

Khiabani

Ghanbarzadeh

et al. 2020

World J Microbiol Biotechnol

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Plasma Functionalized Multiwalled Carbon Nanotubes for Immobilization of Candida antarctica Lipase B: Production of Biodiesel from Methanolysis of Rapeseed Oil

Rastian

Khodadadi

Guo

et al. 2015

Appl Biochem Biotechnol

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Surface modification of multiwalled carbon nanotubes (MWCNTs) through functionalization could improve the characteristics of these nanomaterials as support for enzymes. Carboxylation of MWCNTs (MWCNT-COOH) has been carried out in this study using the dielectric barrier discharge (DBD) plasma reactor through humidified air. The chemical method was also used for further functionalization of the MWCNT-COOH through which the amidation of the surfaces with either butylamine (MWCNT-BA) or octadecylamine (MWCNT-OA) was performed. By immobilization of Candida antarctica B lipase (CALB) on these nanoparticles, performance of the immobilized enzyme in catalyzing methanolysis of rapeseed oil was evaluated. The CALB loading on the MWCNT-BA and MWCNT-COOH was 20 mg protein/g, while the value for MWCNT-OA was 11 mg protein/g. The yield of biodiesel was determined as percentage of mass of fatty acid methyl ester (FAME) produced per initial mass of the oil, and the yield value for the two of these three supports namely, MWCNT-COOH and MWCNT-BA used for the CALB immobilization was similar at about 92 %, while 86 % was the yield for the reaction catalyzed by the lipase immobilized on MWCNT-OA. Thermal stability of the immobilized CALB and the catalytic ability of the enzyme in the repeated batch experiments have also been determined.

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Methods for Lipase Immobilization and Their Use for Biodiesel Production from Vegetable Oil

Cited by 9 publications

References 7 publications

Immobilization of Lipozyme TL 100L for methyl esterification of soybean oil deodorizer distillate

Immobilization of Lipozyme TL 100L for methyl esterification of soybean oil deodorizer distillate

Enhancement of biochemical aspects of lipase adsorbed on halloysite nanotubes and entrapped in a polyvinyl alcohol/alginate hydrogel: strategies to reuse the most stable lipase

Plasma Functionalized Multiwalled Carbon Nanotubes for Immobilization of Candida antarctica Lipase B: Production of Biodiesel from Methanolysis of Rapeseed Oil

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