Methods for lipase detection and assay: a critical review

Beisson, Frédéric; Tiss, Ali; Rivière, C.; Verger, Robert

doi:10.1002/(sici)1438-9312(200002)102:2<133::aid-ejlt133>3.0.co;2-x

Cited by 327 publications

(185 citation statements)

References 183 publications

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“…Measurement of lipase activity requires a method for determining the amount of these released fatty acids. As can be seen in the literature, there are many procedures available for assaying the hydrolytic activity of lipase 9,10 . The most widely used assays for measuring the free fatty acids librated during the enzymatic hydrolysis of the triglycerides are the titrimetric method and the colori-benzene.…”

Section: Introductionmentioning

confidence: 99%

A Sensitive Microplate Assay for Lipase Activity Measurement Using Olive Oil Emulsion Substrate: Modification of the Copper Soap Colorimetric Method

2016

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Section: Introductionmentioning

confidence: 99%

A Sensitive Microplate Assay for Lipase Activity Measurement Using Olive Oil Emulsion Substrate: Modification of the Copper Soap Colorimetric Method

2016

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“…Industrial lipases are mainly produced from microorganisms that secrete extracellular lipase 4 . Microbial lipases are usually produced by submerged fermentation and their activities are infl uenced by the type and concentration of carbon/nitrogen sources, pH and temperature 5 . Lipases can also be obtained from extremophiles.…”

Section: Introductionmentioning

confidence: 99%

Enzymatic activity of a novel halotolerant lipase from Haloarcula hispanica 2TK2

Ozgen

Attar

Elalmış

et al. 2016

Polish Journal of Chemical Technology

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A strain of Haloarcula hispanica isolated from Tuzkoy salt mine, Turkey exhibited extracellular lipolytic activity. Important parameters such as carbon sources and salt concentration for lipase production were investigated. Optimal conditions for the enzyme production from Haloarcula hispanica 2TK2 were determined. It was observed that the lipolytic activity of Haloarcula hispanica was stimulated by some of the carbon sources. The high lipase acitivity values were obtained in the presence of 2% (v/v) walnut oil (6.16 U/ml), 1% (v/v) fi sh oil (5.07 U/ml), 1% (v/v) olive oil (4.52 U/ml) and 1% (w/v) stearic acid (4.88 U/ml) at 4M NaCl concentration. Lipase was partially purifi ed by ammonium sulfate precipitation and ultrafi ltration. Optimal temperature and pH values were determined as 45 o C and 8.0, respectively. Lipase activity decreased with the increasing salt concentration, but 85% activity of the enzyme was maintained at 5M NaCl concentration. The enzyme preserved 41% of its relative activity at 90 o C. The partially purifi ed lipase maintained its activity in the presence of surfactants such as Triton X-100 and SDS. Therefore, the lipase which is an extremozyme may have potential applications especially in detergent industry.

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“…Triglyceride, the main component of natural oil or fat, is stepwise converted into diacylglycerol, monoacylglycerol and glycerol by hydrolysis accompanied with the liberation of a fatty acid at each step [1]. Glycerol and fatty acid are widely used as raw materials, and monoacylglycerol is used as an emulsifying agent in the food, cosmetic and pharmaceutical industries [2].…”

Section: Introductionmentioning

confidence: 99%

Kinetic Model for Triglyceride Hydrolysis Using Lipase: Review

Hermansyah

Wijanarko

2010

MST

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Triglyceride hydrolysis using lipase has been proposed as a novel method to produce raw materials in food and cosmetic industries such as diacylglycerol, monoacylglycerol, glycerol and fatty acid. In order to design a reactor for utilizing this reaction on industrial scale, constructing a kinetic model is important. Since the substrates are oil and water, the hydrolysis takes place at oil-water interface. Furthermore, the triglyceride has three ester bonds, so that the hydrolysis stepwise proceeds. Thus, the reaction mechanism is very complicated. The difference between the interfacial and bulk concentrations of the enzyme, substrates and products, and the interfacial enzymatic reaction mechanism should be considered in the model.

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Methods for lipase detection and assay: a critical review

Cited by 327 publications

References 183 publications

A Sensitive Microplate Assay for Lipase Activity Measurement Using Olive Oil Emulsion Substrate: Modification of the Copper Soap Colorimetric Method

A Sensitive Microplate Assay for Lipase Activity Measurement Using Olive Oil Emulsion Substrate: Modification of the Copper Soap Colorimetric Method

Enzymatic activity of a novel halotolerant lipase from Haloarcula hispanica 2TK2

Kinetic Model for Triglyceride Hydrolysis Using Lipase: Review

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