A Sensitive Microplate Assay for Lipase Activity Measurement Using Olive Oil Emulsion Substrate: Modification of the Copper Soap Colorimetric Method

Mustafa, Azeman; Karmali, Amin; Abdelmoez, Wael

doi:10.5650/jos.ess16066

Cited by 21 publications

(7 citation statements)

References 20 publications

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“…In this method, proteases split peptide bonds, and casein acts as a substrate. Lipase activity was assayed, according to Mustafa et al (2016). Experiments were carried out in triplicate, and the standard deviations from the mean values were calculated.…”

Section: Digestive Enzyme Analysismentioning

confidence: 99%

Singular effects of Bacillus subtilis C-3102 or Saccharomyces cerevisiae type 1 on the growth, gut morphology, immunity, and stress resistance of red sea bream (Pagrus major)

Zaineldin

Hegazi

Koshio

et al. 2021

Annals of Animal Science

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The beneficial effects of Bacillus subtilis C-3102 and Saccharomyces cerevisiae type 1 were tested in red sea bream (Pagrus major) feeds. A basal diet (control) and two other diets were prepared by supplementation with B. subtilis C-3102 (PB) or S. cerevisiae type 1 (PY). After 60 days, both probiotic-supplemented groups exhibited significant enhancement in growth performance, the protein efficiency ratio (PER), and digestive enzyme secretion (protease and amylase) compared to the control group (P<0.05). The anterior, middle, and posterior parts of the intestines exhibited significantly increased values of intestinal fold height (hF), enterocyte height (hE), and microvillus height (hMV) in fish fed PB- or PY-supplemented diets (P<0.05). Serum peroxidase, anti-protease, and bactericidal activities were enhanced significantly in both probiotic-treated groups compared to the control group (P<0.05). Serum and mucus lysozyme activities improved significantly in the PB group compared to the control group (P<0.05). Catalase activity was also significantly decreased in both probiotic groups, with relatively lower activity observed in the PY group (P<0.05). Both probiotic groups showed increased tolerance considerably to freshwater exposure (P<0.05). In conclusion, B. subtilis C-3102 and S. cerevisiae type 1 can be used as functional probiotics to enhance the growth performance, digestion capacity, gut morphology, immune response, and stress resistance of the red sea bream with relatively higher efficiency by B. subtilis C-3102.

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Section: Digestive Enzyme Analysismentioning

confidence: 99%

Singular effects of Bacillus subtilis C-3102 or Saccharomyces cerevisiae type 1 on the growth, gut morphology, immunity, and stress resistance of red sea bream (Pagrus major)

Zaineldin

Hegazi

Koshio

et al. 2021

Annals of Animal Science

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show abstract

“…Lipase activity was analysed following Mustafa, Karmali, and Abdelmoez (), with slight modifications. Olive oil emulsion was prepared by mixing 50 ml of olive oil with 150 ml 4% polyvinyl alcohol solution in a homogenizer.…”

Section: Methodsmentioning

confidence: 99%

Effects of dietary astaxanthin and vitamin E and their interactions on the growth performance, pigmentation, digestive enzyme activity of kuruma shrimp (Marsupenaeus japonicus )

et al. 2019

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A 56‐day feeding trial was done to investigate the interactive effects of astaxanthin (Ax) and vitamin E (α‐Toc) on the performance of kuruma shrimp (Marsupenaeus japonicus). A 2 × 3 factorial experiment was conducted with six experimental diets containing two levels of Ax (0 and 0.6 g/kg diet [Ax0 and Ax0.6]) and three levels of α‐Toc (0, 0.2 and 1 g/kg diet [α‐Toc0, α‐Toc0.2 and α‐Toc1]). The results indicated that growth performance was significantly (p < 0.05) increased in shrimp fed with the Ax0.6 × α‐Toc0.2 diet. Interactive effects between Ax and α‐Toc on the growth parameters were observed. Furthermore, pigmentation performance was significantly (p < 0.05) better in the Ax0.6 groups. Interaction between Ax and α‐Toc was also found in the Ax content of shrimp body parts. Interestingly, dietary α‐Toc helped to reduce the Ax consumption rate, promote the absorption and increase the deposition of Ax in the muscle. Shrimps from the Ax0.6 groups showed significantly (p < 0.05) improved hepatopancreatic digestive enzyme activities compared with those of the Ax0 groups. Although no interactive (p > 0.05) effects were found between dietary α‐Toc and Ax on total haemocyte count and tolerance against freshwater, dietary Ax and α‐Toc supplementation showed better performance on these two parameters. It was concluded that dietary Ax and α‐Toc functioned synergistically, and the shrimp fed with the diet containing 0.6 g Ax/kg diet Ax and 0.2 g α‐Toc/kg diet showed improved growth and pigmentation performance compared with the other groups in the current study.

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“…Protease activity was analysed with the method of Sigma's Non‐specific Protease Activity Assay using casein as the substrate and reacting with Folin‐phenol reagent (Cupp‐Enyard, ). Lipase activity was analysed by using olive oil emulsion as the substrate according to Mustafa, Karmali, and Abdelmoez (). Amylase activity was analysed by using starch as the substrate according to Shiu et al () and Wang et al ().…”

Section: Methodsmentioning

confidence: 99%

Interactive effects of dietary astaxanthin and cholesterol on the growth, pigmentation, fatty acid analysis, immune response and stress resistance of kuruma shrimp ( Marsupenaeus japonicus )

et al. 2019

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A 56‐day feeding trial was carried out to evaluate the interactive effects of chemically synthesized astaxanthin (Ax) and cholesterol (CHO) on the performances of kuruma shrimp juveniles. Six experimental diets containing 2 levels of Ax (0 and 0.6 g/kg diet) and 3 levels of CHO (0, 6 and 20 g/kg diet) were formulated in 2 × 3 factorial design. The results indicated that the interactions between dietary CHO and Ax were not significant on survival, growth performances, body proximate analysis, immune response and freshwater stress test. Significantly, better pigmentation performances and Ax contents of different parts of juveniles (whole body, muscle, carapace and head) were observed in higher Ax (Ax0.6)‐supplemented groups. Meanwhile, interactions of dietary Ax and CHO were also found on colour reading parameters and whole‐body Ax contents. In addition, juveniles fed with the diet containing Ax at 0.6 g/kg diet in addition to CHO at 0, 6 and 20 g/kg diet showed better performances on digestive enzyme activities (protease, lipase and amylase) of hepatopancreas than 0 g Ax/kg diet supplemented with CHO at 0, 6 and 20 g/kg diet. It was concluded that dietary Ax and CHO functioned interactively.

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A Sensitive Microplate Assay for Lipase Activity Measurement Using Olive Oil Emulsion Substrate: Modification of the Copper Soap Colorimetric Method

Cited by 21 publications

References 20 publications

Singular effects of Bacillus subtilis C-3102 or Saccharomyces cerevisiae type 1 on the growth, gut morphology, immunity, and stress resistance of red sea bream (Pagrus major)

Singular effects of Bacillus subtilis C-3102 or Saccharomyces cerevisiae type 1 on the growth, gut morphology, immunity, and stress resistance of red sea bream (Pagrus major)

Effects of dietary astaxanthin and vitamin E and their interactions on the growth performance, pigmentation, digestive enzyme activity of kuruma shrimp (Marsupenaeus japonicus )

Interactive effects of dietary astaxanthin and cholesterol on the growth, pigmentation, fatty acid analysis, immune response and stress resistance of kuruma shrimp ( Marsupenaeus japonicus )

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