Methods for isolation of marine-derived endophytic fungi and their bioactive secondary products

Abstract

Marine-derived fungi have been shown in recent years to produce a plethora of new bioactive secondary metabolites, some of them featuring new carbon frameworks hitherto unprecedented in nature. These compounds are of interest as new lead structures for medicine as well as for plant protection. The aim of this protocol is to give a detailed description of methods useful for the isolation and cultivation of fungi associated with various marine organisms (sponges, algae and mangrove plants) for the extraction, characterization and structure elucidation of biologically active secondary metabolites produced by these marine-derived endophytic fungi, and for the preliminary evaluation of their pharmacological properties based on rapid 'in house' screening systems. Some results exemplifying the positive outcomes of the protocol are given at the end. From sampling in marine environment to completion of the structure elucidation and bioactivity screening, a period of at least 3 months has to be scheduled.

Fungos e aflatoxinas no arroz: ocorrência e significado na saúde do consumidor

“…Um fator preocupante do arroz é que este cereal é um ótimo substrato para a produção de micotoxinas e os fungos as produzem em grandes quantidades (KJER et al, 2010). As micotoxinas que já foram encontradas nas amostras de arroz são: citrinina, luteosquirina, cicloclorotina, islanditoxina, rugulosina, ocratoxina A, aflatoxinas, desoxinivalenol, fumonisinas e zearalenona, entre outras (FREDLUND et al, 2009;AYDIN et al, 2011;ALMEIDA et al, 2012).…”

Antibacterial Activity of Indonesian Sponge Associated Fungi Against Clinical Pathogenic Multidrug Resistant Bacteria

“…This study applied surface sterilization methods from Kjer et al (2010) and Sibero et al (2017) for fungal isolation with several modifications. Firstly, sponge was cut into 3 pieces in size 2 × 1 cm 2 and washed using sterile marine water then was immersed in alcohol 70% for 60 s. After that, sponge was washed using sterile marine water to clean the alcohol residue.…”

Antibacterial Activity of Red Pigment Isolated From Coastal Endophytic Fungi Against Multi-Drug Resistant Bacteria

“…Each fungus had different macroscopic characteristics based on colony forms, mycelia colors and reverse media colors. Biodiversity of culturable fungi is influenced by nutrient content in media and isolation method (Kjer et al 2010;Toma & Abdulla 2013). Surface sterilization is the most important procedure in endophytic fungal isolation.…”

“…Surface sterilization is the most important procedure in endophytic fungal isolation. The aim of surface sterilization using ethanol 70% is to eliminate spores and other microbial contaminants attached to the sample surface (Kjer et al 2010). Environmental control petri dish is also important to minimize the possibility of improper isolation.…”