Methods for In Vitro Percutaneous Absorption Studies V: Permeation Through Damaged Skin

Bronaugh, Robert L.; Stewart, Raymond F.

doi:10.1002/jps.2600741008

Cited by 123 publications

(75 citation statements)

References 11 publications

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“…Experiment 2: Experiment 1 was repeated using an abraded skin protocol as suggested by Bronaugh and Stewart (1985): The skin was abraded by drawing the point of a 19-gauge hypodermic needle across the surface (20 marks in one direction and 20 perpendicular).…”

Section: In Vitro Diffusion Systemmentioning

confidence: 99%

“…Using this technique, we investigated the total amount of gold permeating through human skin during a 24-h period. Experiments were run with intact skin as well as abraded skin following the Bronaugh and Stewart (1985) protocol to estimate the effect of skin lesions on the permeation rate. In this study, we applied the protocol adopted in the European project EDETOX (Evaluations and predictions of DErmal absorption of TOXic chemicals), a three-year research program (2001)(2002)(2003)(2004) financially supported by the European Commission, and already used by our group to study the in vitro dermal absorption of silver nanoparticles (Larese Filon et al 2009a).…”

Section: Introductionmentioning

confidence: 99%

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Human skin penetration of gold nanoparticles through intact and damaged skin

Filon¹,

et al. 2011

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Gold nanoparticles (AuNPs) are produced for many applications but there is a lack of available data on their skin absorption. Experiments were performed using the Franz diffusion cell method with intact and damaged human skin. A physiological solution was used as receiving phase and 0.5 mL (1st exp) and 1.5 mL (2nd exp) of a solution containing 100 mgL and 7.1 ± 2.5 ng cm-2 h -1 in intact and damaged skin, respectively, with a lag time less than 1 hour. Transmission Electron Microscope analysis on skin samples and chemical analysis using Inductively Coupled PlasmaMass Spectrometry demonstrated the presence of AuNPs into epidermis and dermis. This study showed that AuNPs are able to penetrate the human skin in an in vitro diffusion cell system.

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Section: In Vitro Diffusion Systemmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Human skin penetration of gold nanoparticles through intact and damaged skin

Filon¹,

et al. 2011

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“…Porcine skin disks, dermatomed from fresh skin to a thickness of 500 μm and presenting an exposed surface of 0.32 cm 2 , were used as barrier membranes in a flow-through diffusion cell system according to the methodology of (Bronaugh & Stewart 1985), as adapted by (Chang & Riviere 1991 5.50 g NaHCO 3 , 2.40 g dextrose, 90.0 g bovine serum albumin, 0.25 ml amikacin, 10 ml heparin and 0.1 ml penicillin G sodium made up to 2 l with glass distilled water. An 8 h experimental period was used, which allowed the estimation of adequate flux/time curves while avoiding complications due to skin degradation.…”

Section: Permeabilitymentioning

confidence: 99%

Effect of vehicles and sodium lauryl sulphate on xenobiotic permeability and stratum corneum partitioning in porcine skin

Merwe

Riviere

2005

Toxicology

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Dermal contact with potentially toxic agricultural and industrial chemicals is a common hazard encountered in occupational, accidental spill and environmental contamination scenarios. Different solvents and chemical mixtures may influence dermal absorption. The effects of sodium lauryl sulphate (SLS) on the stratum corneum partitioning and permeability in porcine skin of 10 agricultural and industrial chemicals in water, ethanol and propylene glycol were investigated. The chemicals were phenol, p-nitrophenol, pentachlorophenol, methyl parathion, ethyl parathion, chlorpyrifos, fenthion, simazine, atrazine and propazine. SLS decreased partitioning into stratum corneum from water for lipophilic compounds, decreased partitioning from propylene glycol and did not alter partitioning from ethanol. SLS effects on permeability were less consistent, but generally decreased permeability from water, increased permeability from ethanol and had an inconsistent effect on permeability from propylene glycol. It was concluded that, for the compounds tested, partitioning into the stratum corneum was determined by the relative solubility of the solute in the donor solvent and the stratum corneum lipids.Permeability, however, reflected the result of successive, complex processes and was not predictable from stratum corneum partitioning alone. Addition of SLS to solvents altered partitioning and absorption characteristics across a range of compounds, which indicates that partition coefficients or skin permeability from neat chemical exposure should be used with caution in risk assessment procedures for chemical mixtures.

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“…Tape-stripping was repeated until the surface of the skin had a glistening ap pearance and light erythema, indicating essentially all of the Stratum corneum to have been removed. 13,14) In the single dose study, the patch applied to intact or damaged skin was removed at 24 hr following application and ex cess unabsorbed test compond was removed with sanita ry cotton. In repetitive dermal applications, fresh patch es were used every 24 hr for 7 days.…”

Section: Animal Treatmentmentioning

confidence: 99%

Percutaneous Absorption and Tissue Distribution of 1-(3,4-dichlorobenzyl)-5-octylbiguanide (OPB-2045) in Rats.

Kudoh

Furukawa

Okumura

et al. 1998

Drug Metabolism and Pharmacokinetics

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Summary: The absorption and tissue distribution of OPB-2045, a new antiseptic antimicrobial drug with a biguanide moiety in the molecule, were studied in male Sprague-Dawley rats with intact or damaged skin fol lowing a single and repetitive dermal application of 0.1% 14C-OPB-2045 in a liquid formulation applied at the dose of 0.3 mg/rat to 6.25 cm2 of the skin. Comparative percutaneous absorption was also investigated in rats treated with single doses of 0.04% 14C-OPB-2045 and 14C-chlorhexidine in aqueous solution at 0.12 mg/rat to the same area of the skin.Radioactivity was hardly detected in the serum of rats with intact and damaged skin during a 24-hr period after single topical application of 0.1% 14C-OPB-2045. Urinary and fecal excretions of radioactivity within 336 hr after single dosing were 1.20/ and 4.1% of the dose, respectively, in rats with intact skin, and 2.4% and 5.0%, respectively, in rats with damaged skin, indicating no significant differences. Percutaneous absorption was estimated to be approximately 1.40/ of the total dose in rats with intact skin following repeti tive dermal application of the compound. Radioactivity in various tissues was quite low except the applica tion site of the compound, and the disappearance of radioactivity from the application site was sluggish in in tact and damaged skin rats. Percutaneous absorption of 14C-OPB-2045 and 14C-chlorhexidine in aqueous so lution was 4.20/ and 4.2% of the dose respectively in intact skin, and 7.1% and 3.8% respectively in damaged skin. The percutaneous absorption of OPB-2045 is thus quite poor, and essentially the same to chlorhexidine. Stratum corneum, an important tissue as a barrier controlling percutaneous absorption of chemicals does not appear to be important to the absorption of either OPB-2045 or chlorhexidine through the skin.

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Methods for In Vitro Percutaneous Absorption Studies V: Permeation Through Damaged Skin

Cited by 123 publications

References 11 publications

Human skin penetration of gold nanoparticles through intact and damaged skin

Human skin penetration of gold nanoparticles through intact and damaged skin

Effect of vehicles and sodium lauryl sulphate on xenobiotic permeability and stratum corneum partitioning in porcine skin

Percutaneous Absorption and Tissue Distribution of 1-(3,4-dichlorobenzyl)-5-octylbiguanide (OPB-2045) in Rats.

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