Methods for animal cell immobilization using electrostatic droplet generation

Bugarski, Branko; Smith, Jodi-Anne; Wu, Jianyong; Goosen, Mattheus F. A.

doi:10.1007/bf00151869

Cited by 33 publications

(13 citation statements)

References 5 publications

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“…Hommel et al (1988) proposed replacing the continuous voltage by very short pulses (1 to 6 ms) with a controlled frequency (10 to lOO/s) in order to obtain beads in the range of 100 to 200 pm. Bugarski et al (1993) demonstrated alginate microbead production (450 pm) by electrostatic spraying. In this case, pulsed voltage was not necessary.…”

Section: Extrusion Under Electrostatic Potentialmentioning

confidence: 99%

Immobilization of Cells for Application in the Food Industry

Groboillot

Boadi

Poncelet

et al. 1994

Critical Reviews in Biotechnology

165

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Immobilization of cells offers advantages to the food process industries, including enhanced fermentation productivity and cell stability and reduced downstream processing costs due to facilitated cell recovery and recycle. This article summarizes the varied immobilization methodologies, including adsorption, entrapment, covalent binding, and microencapsulation. Examples of interest to the food industry are provided, together with a review of the physiological effects of immobilization. Topics in process engineering include immobilized cell bioreactor configurations and the scale-up potential of the various immobilization techniques.

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Section: Extrusion Under Electrostatic Potentialmentioning

confidence: 99%

Immobilization of Cells for Application in the Food Industry

Groboillot

Boadi

Poncelet

et al. 1994

Critical Reviews in Biotechnology

165

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“…Electrostatic extrusion is a novel, proven technique for immobilization of various cell types, such as brewing yeast cells (Nedovic et al 2001a, 2001b, 2002), insect cells (Bugarski et al 1993, 1994), and plant cells (Sajc et al 1995). The advantages of electrostatic extrusion make the technique a first choice for immobilization procedures in medicine.…”

Section: Introductionmentioning

confidence: 99%

Immobilization of cells by electrostatic droplet generation: a model system for potential application in medicine

Manojlović¹,

Djonlagić²,

Obradović³

et al. 2006

International Journal of Nanomedicine

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Abstract:The process of electrostatic extrusion as a method for cell immobilization was investigated that could be used for potential applications in medicine. An attempt was made to assess the effects of cell addition and polymer concentration on the overall entrapment procedure, ie, on each stage of immobilization: polymer-cell suspension rheological characteristics, electrostatic extrusion process, and the process of gelation. The findings should contribute to a better understanding of polymer-cell interactions, which could be crucial in possible medical treatments. Alginate-yeast was used as a model system for carrier-cells. The electrostatic extrusion was considered as a complex two-phase flow system and the effects of cell and alginate concentrations on the resulting microbead size and uniformity were assessed. Under investigated conditions, microbeads 50-600 µm in diameter were produced and the increase in both alginate and cell concentrations resulted in larger microbeads with higher standard deviations in size. We attempted to rationalize the findings by rheological characterization of the cell-alginate suspensions. Rheological characterization revealed non-Newtonian, pseudoplastic behavior of cell-alginate suspensions with higher viscosities at higher alginate concentrations. However, the presence of cells even at high concentrations (5x10 8 and 1x10 9 cells/mL) did not significantly affect the rheological properties of Na-alginate solution. Lastly, we investigated the kinetics of alginate gelation with respect to the quantity of Ca 2+ ions and cell presence. The gelation kinetics were examined under conditions of limited supply with Ca 2+ ions, which can be essential for immobilization of highly sensitive mammalian cells that require minimal exposure to CaCl 2 solution. The molar ratio of G units to Ca 2+ ions of 3.8:1 provided complete crosslinking, while the increase in alginate concentration resulted in prolonged gelation times but higher strength of the resulting gel. The cell presence decreased the rate of network formation as well as the strength of the obtained Ca-alginate hydrogel.

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“…Few attempts though have been made in the application of electric fields to the production of micron-sized polymer beads for cell immobilization . In recent articles (Bugarski et al, 1993(Bugarski et al, , 1994a, the mechanism of droplet formation using an electrostatic droplet generator was investigated with a variable-voltage power supply. Animal cell suspensions were successfully extruded using the electrostatic droplet generator.…”

Section: Introductionmentioning

confidence: 99%

Electrostatic Encapsulation and Growth of Plant Cell Cultures in Alginate

et al. 1999

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The growth of callus tissue from African Violets, encapsulated in alginate using electrostatics, was investigated as well as the mechanism of alginate droplet formation. Alginate microbeads as small as 500 (+/-50) microns in diameter could be produced by electrostatic extrusion directly from a plastic syringe (1900 micron extrusion orifice), in the absence of a needle. Video analysis of the mechanism of electrostatic alginate droplet formation from the syringe showed the development of a Taylor cone-like droplet which extended to form a thin strand that then broke up into droplets. Autoclaving of the alginate/medium solution significantly reduced its viscosity, giving smaller beads. Calculated microbead diameters agreed well with experimental values. Callus tissue from leaf explants was successfully immobilized and cultured using electrostatic extrusion. Tissue immobilized using 4% alginate in medium and cultured on agar grew best, producing a complete plantlet within four months. The long-term aim is to develop an effective method for large production of artificial seeds.

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Methods for animal cell immobilization using electrostatic droplet generation

Cited by 33 publications

References 5 publications

Immobilization of Cells for Application in the Food Industry

Immobilization of Cells for Application in the Food Industry

Immobilization of cells by electrostatic droplet generation: a model system for potential application in medicine

Electrostatic Encapsulation and Growth of Plant Cell Cultures in Alginate

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