Methodological Improvements for the Recovery of B. fragilis Phages and Coliphages from Environmental Samples

Araujo, Rosa; Lasobras, J.; Lucena, F.; Jofre, Joan

doi:10.2166/wst.1993.0333

Cited by 8 publications

(3 citation statements)

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“…The low removal rate by this pre-treatment showed that it may be a promise biological indicator for drinking water supply. The MPN method appeared to be more sensible than that of PFU as reported previously by Ajaujo et al (1993) and Tartera et al (1988Tartera et al ( , 1992. (1995).…”

Section: Resultssupporting

confidence: 71%

Quantification of bacteriophages ofBacteroides fragilis in environmental water samples of Seine River

Sun¹,

Lévi²,

Kiéné³

et al. 1997

Water Air Soil Pollut

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Virus detecnon in drinking water is very important to protect human health. The different methodologies for analysing human pathogenic virus are very time consuming and expensive, so until now only a few specialised laboratories carried out this analysis. Detection of bacteriophages may be possible by examining the aquatic virus, with advantages of easy and cheap. The bacteriophages of Bacteroidesfragilis have been proven as specifically present in human faeces and have relationships with water contaminated by enterovirus. Our study, for the first time in France. discovered the B.fragilis phages present in sample of sewage. Seine river and raw water for water supply. Our results also presented that B, fragilis phages may be a better indicator for water bacteriology compared with classical bacteriological indicators in water treatment. On the other hand, our results demonstrated that MPN method (Most Probable Number) has more advantages than that of PFU (Plaque Forming Units).

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Section: Resultssupporting

confidence: 71%

Quantification of bacteriophages ofBacteroides fragilis in environmental water samples of Seine River

Sun¹,

Lévi²,

Kiéné³

et al. 1997

Water Air Soil Pollut

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“…The sodium pyrophosphate (10 mM) solution (pH 7) used was that described by Danovaro et al (17). The glycine (250 mM) solution (pH 8) used was that described by Araujo et al (2).…”

Section: Methodsmentioning

confidence: 99%

Sampling Natural Viral Communities from Soil for Culture-Independent Analyses

Williamson

Wommack

Radosevich

2003

Appl Environ Microbiol

153

122

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An essential first step in investigations of viruses in soil is the evaluation of viral recovery methods suitable for subsequent culture-independent analyses. In this study, four elution buffers (10% beef extract, 250 mM glycine buffer, 10 mM sodium pyrophosphate, and 1% potassium citrate) and three enumeration techniques (plaque assay, epifluorescence microscopy [EFM], and transmission electron microscopy [TEM]) were compared to determine the best method of extracting autochthonous bacteriophages from two Delaware agricultural soils. Beef extract and glycine buffer were the most effective in eluting viable phages inoculated into soils (up to 29% recovery); however, extraction efficiency varied significantly with phage strain. Potassium citrate eluted the highest numbers of virus-like particles from both soils based on enumerations by EFM (mean, 5.3 ؋ 10 8 g of dry soil ؊1 ), but specific soil-eluant combinations posed significant problems to enumeration by EFM. Observations of virus-like particles under TEM gave confidence that the particles were, in fact, phages, but TEM enumerations yielded measurements of phage abundance (mean, 1.5؋10 8 g of dry soil ؊1 ) that were about five times lower. Clearly, the measurement of phage abundance in soils varies with both the extraction and enumeration methodology; thus, it is important to assess multiple extraction and enumeration approaches prior to undertaking ecological studies of phages in a particular soil.

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“…(1993) presented a modified analysis for determining the bacterial count in activated sludge solids. The recovery of Bacteroides fragilis from sludge and sediment was improved by treatment with glycine followed by titration (Araujo et al, 1993). More than 90% of copper (II) in aqueous solution was removed by biomass obtained from activated sludge (Nagendra Rao et al, 1993).…”

Section: Sludge Propertiesmentioning

confidence: 99%