Methodological Approaches to Assessing the Size and Morphology of Microvesicles of Cell Lines

Маркова, К. Л.; Kozyreva, A. R.; Gorshkova, A. A.; Александрова, Е. П.; Berezkina, M. E.; Михайлова, В. А.; Ivanova, Alena; Kaputkina, S. Yu.; Onokhin, K. V.; Benken, Konstantin A.; Selkov, S. A.; Соколов, Д. И.

doi:10.1007/s10517-020-04934-2

Cited by 4 publications

(4 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The size of MVs derived from NK cells of the NK-92 cell line ranged from 210 nm to 490 nm, and the peak of the MV quantity distribution was 315 nm. These data complied with our previous work [65,66] and that of other research groups that ascertained the size of MVs derived from various cells [6,45,67]. The addition of IL-1β did not change the average size of MVs.…”

Section: Analysis By Dynamic Light Scatteringsupporting

confidence: 91%

Effects of Microvesicles Derived from NK Cells Stimulated with IL-1β on the Phenotype and Functional Activity of Endothelial Cells

Маркова

Михайлова

Milyutina

et al. 2021

IJMS

View full text Add to dashboard Cite

Microvesicles (MVs) are plasma extracellular vesicles ranging from 100 (150) to 1000 nm in diameter. These are generally produced by different cells through their vital activity and are a source of various protein and non-protein molecules. It is assumed that MVs can mediate intercellular communication and modulate cell functions. The interaction between natural killer cells (NK cells) and endothelial cells underlies multiple pathological conditions. The ability of MVs derived from NK cells to influence the functional state of endothelial cells in inflammatory conditions has yet to be studied well. In this regard, we aimed to study the effects of MVs derived from NK cells of the NK-92 cell line stimulated with IL-1β on the phenotype, caspase activity, proliferation and migration of endothelial cells of the EA.hy926 cell line. Endothelial cells were cultured with MVs derived from cells of the NK-92 cell line after their stimulation with IL-1β. Using flow cytometry, we evaluated changes in the expression of endothelial cell surface molecules and endothelial cell death. We evaluated the effect of MVs derived from stimulated NK cells on the proliferative and migratory activity of endothelial cells, as well as the activation of caspase-3 and caspase-9 therein. It was established that the incubation of endothelial cells with MVs derived from cells of the NK-92 cell line stimulated with IL-1β and with MVs derived from unstimulated NK cells, leads to the decrease in the proliferative activity of endothelial cells, appearance of the pan leukocyte marker CD45 on them, caspase-3 activation and partial endothelial cell death, and reduced CD105 expression. However, compared with MVs derived from unstimulated NK cells, a more pronounced effect of MVs derived from cells of the NK-92 cell line stimulated with IL-1β was found in relation to the decrease in the endothelial cell migratory activity and the intensity of the CD54 molecule expression on them. The functional activity of MVs is therefore mediated by the conditions they are produced under, as well as their internal contents.

show abstract

Section: Analysis By Dynamic Light Scatteringsupporting

confidence: 91%

Effects of Microvesicles Derived from NK Cells Stimulated with IL-1β on the Phenotype and Functional Activity of Endothelial Cells

Маркова

Михайлова

Milyutina

et al. 2021

IJMS

View full text Add to dashboard Cite

show abstract

“…The analysis of MVs using dynamic light scattering showed that the size of most of the particles was within 180-250 nm (Figure 1B). The STEM image (Figure 1C) shows that the diameter of most MVs was 180 nm, aligning with the data previously obtained for MVs isolated from the same cells [16].…”

Section: Isolation and Imaging Of Mvssupporting

confidence: 90%

Surface Glycans of Microvesicles Derived from Endothelial Cells, as Probed Using Plant Lectins

Slivka,

Shilova,

Obraztsova

et al. 2024

IJMS

View full text Add to dashboard Cite

Glycans of MVs are proposed to be candidates for mediating targeting specificity or at least promoting it. In contrast to exosomes, glycomic studies of MVs are largely absent. We studied the glycoprofile of endothelial cell-derived MVs using 21 plant lectins, and the results show the dominance of oligolactosamines and their α2-6-sialylated forms as N-glycans and low levels of α2-3-sialylated glycans. The low levels of α2-3-sialosides could not be explained by the action of extracellular glycosidases. Additionally, the level of some Man-containing glycans was also decreased in MVs. Spatial masking as the causative relationship between these low level glycans (as glycosphingolipids) by integral proteins or proteoglycans (thus, their lack of interaction with lectins) seems unlikely. The results suggest that integral proteins do not pass randomly into MVs, but instead only some types, differing in terms of their specific glycosylation, are integrated into MVs.

show abstract

“…Microvesicles are produced by cells both at rest and in the state of activation, differing in their composition and their effect on target cells [ 16 , 18 , 68 , 80 , 81 , 82 , 83 ]. Several types of interactions of vesicles with target cells are described that help vesicles deliver their contents: caveolin- and clathrin-induced endocytosis, macropinocytosis [ 84 ], endocytosis of lipid rafts [ 85 ], and phagocytosis [ 86 , 87 , 88 ].…”

Section: Discussionmentioning

confidence: 99%

“…This supports the involvement of extracellular MVs in intercellular interactions and as a component of the mechanism of intercellular communication for the maintenance of physiological homeostasis in the body [ 15 ]. Various types of cells produce MVs, including macrophages and natural killer cells [ 16 , 17 , 18 ], trophoblast cells [ 18 , 19 ], dendritic cells, megakaryocytes, platelets, endothelial and epithelial cells, nerve cells, tumor cells, and stem cells [ 1 ].…”

Section: Introductionmentioning

confidence: 99%

Natural Killer Cell Derived Microvesicles Affect the Function of Trophoblast Cells

et al. 2023

View full text Add to dashboard Cite

The interaction of natural killer (NK) and trophoblast cells underlies the formation of immune tolerance in the mother–fetus system and the maintenance of the physiological course of pregnancy. In addition, NK cells affect the function of trophoblast cells, interacting with them via the receptor apparatus and through the production of cytokines. Microvesicles (MVs) derived from NK cells are able to change the function of target cells. However, in the overall pattern of interactions between NK cells and trophoblasts, the possibility that both can transmit signals to each other via MVs has not been taken into account. Therefore, the aim of this study was to assess the effect of NK cell-derived MVs on the phenotype, proliferation, and migration of trophoblast cells and their expression of intracellular messengers. We carried out assays for the detection of content transferred from MV to trophoblasts. We found that NK cell-derived MVs did not affect the expression of CD54, CD105, CD126, CD130, CD181, CD119, and CD120a receptors in trophoblast cells or lead to the appearance of CD45 and CD56 receptors in the trophoblast membrane. Further, the MVs reduced the proliferation but increased the migration of trophoblasts with no changes to their viability. Incubation of trophoblast cells in the presence of MVs resulted in the activation of STAT3 via pSTAT3(Ser727) but not via pSTAT3(Tyr705). The treatment of trophoblasts with MVs did not result in the phosphorylation of STAT1 and ERK1/2. The obtained data indicate that NK cell-derived MVs influence the function of trophoblast cells, which is accompanied by the activation of STAT3 signaling.

show abstract

Methodological Approaches to Assessing the Size and Morphology of Microvesicles of Cell Lines

Cited by 4 publications

References 29 publications

Effects of Microvesicles Derived from NK Cells Stimulated with IL-1β on the Phenotype and Functional Activity of Endothelial Cells

Effects of Microvesicles Derived from NK Cells Stimulated with IL-1β on the Phenotype and Functional Activity of Endothelial Cells

Surface Glycans of Microvesicles Derived from Endothelial Cells, as Probed Using Plant Lectins

Natural Killer Cell Derived Microvesicles Affect the Function of Trophoblast Cells

Contact Info

Product

Resources

About