Method Validation by Interlaboratory Studies of Improved Hydrophilic Oxygen Radical Absorbance Capacity Methods for the Determination of Antioxidant Capacities of Antioxidant Solutions and Food Extracts

Watanabe, Jun; Oki, Tomoyuki; Takebayashi, Jun; Yamasaki, Koji; Takano-Ishikawa, Yuko; Hino, Akihiro; Yasui, Akemi

doi:10.2116/analsci.28.159

Cited by 90 publications

(71 citation statements)

References 15 publications

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“…ORAC assay was performed in accordance with the method described by Watanabe et al [14]. All measurements were obtained using a plate reader (EnSpire; PerkinElmer, Inc., Wellesley, MA, USA).…”

Section: Antioxidant Activitiesmentioning

confidence: 99%

Preliminary study on antioxidant properties, phenolic contents, and effects of Aesculus hippocastanum (horse chestnut) seed shell extract on in vitro cyclobutane pyrimidine dimer repair

Makino¹,

Katšube²,

Ohta³

et al. 2017

J Intercult Ethnopharmacol

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Aim: Human skin exposure to solar ultraviolet (UV) radiation induces cells to produce reactive oxygen species that directly cause DNA damage via the formation of cyclobutane pyrimidine dimers (CPDs), which are the primary UVB-induced DNA lesions. CPDs are responsible for cell death, mutation, and neoplastic transformation. Aesculus hippocastanum (horse chestnut) seed extract has traditionally been used for venotonic treatments and also as a raw material for cosmetics. This study aimed to characterize the antioxidant properties of the phenolic contents of extracts from A. hippocastanum seed shell and endosperm and to investigate their effects on CPD repair in UVB-exposed human dermal fibroblasts in vitro. Materials and Methods: Crude 60% aqueous ethanol extracts (v/v) were prepared, and their total polyphenol contents, antioxidant activities, and oxygen radical absorbance capacity (ORAC) values were measured by Folin-Ciocalteu, 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging capacity, and ORAC assays, respectively. The levels of CPDs induced by UVB irradiation were measured by cell-based immunoassays after 6 h treatment with the extract of A. hippocastanum seed shell at a concentration of 100 µg/ml and 9 h treatment with it at concentrations of 50, 100, and 200 µg/ml. Results: A. hippocastanum seed shell extract had 602 ± 1.6 mg gallic acid equivalent per gram of extract and exhibited 4990 ± 70.9 and 7140 ± 835 µmol Trolox equivalents per gram of extract as DPPH radicalscavenging activity and ORAC value, respectively; these values were comparable to those of extracts from green tea and Mallotus japonicus leaves. However, these values of the seed endosperm extract were relatively low. A 9 h treatment of cells with seed shell extracts at concentrations of 50, 100, and 200 µg/ml after UVB exposure significantly reduced CPD levels compared with those in UVB-exposed cells without treatment. Conclusion: This study indicated that A. hippocastanum seed shell extract possesses a potential to limit the harmful effects of human skin exposure to UVB and that this could be the first step toward identifying novel benefits of this extract.

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Section: Antioxidant Activitiesmentioning

confidence: 99%

Preliminary study on antioxidant properties, phenolic contents, and effects of Aesculus hippocastanum (horse chestnut) seed shell extract on in vitro cyclobutane pyrimidine dimer repair

Makino¹,

Katšube²,

Ohta³

et al. 2017

J Intercult Ethnopharmacol

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show abstract

“…The stabilities of the antioxidant solutions and food extracts were verified as previously described 11 prior to the interlaboratory study. The results showed no significant changes occurred during storage at 4 C for 4 weeks.…”

Section: Stability Of the Test Samplesmentioning

confidence: 99%

“…11 In an earlier study of L-ORAC assays, 12 we revealed that the repeatability and intermediate precision of the original L-ORAC method 13 are inadequate, and thus we developed a modified method that provides satisfactory repeatability and intermediate precision in a single-laboratory validation study. Here, we conducted interlaboratory tests in accordance with an internationally harmonized protocol for the design of method-performance studies 14 to validate this improved L-ORAC method.…”

Section: Introductionmentioning

confidence: 99%

Improvement and Interlaboratory Validation of the Lipophilic Oxygen Radical Absorbance Capacity: Determination of Antioxidant Capacities of Lipophilic Antioxidant Solutions and Food Extracts

et al. 2016

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A lipophilic oxygen radical absorbance capacity (L-ORAC) assay is an evaluation of the antioxidant capacity of solutions of lipophilic compounds. The concentrations of fluorescein, radical generator, and Trolox standard solutions were optimized to improve the precision of the assay. An interlaboratory study using two antioxidant solutions and three food extracts as test samples conducted in accordance with harmonized protocol demonstrated satisfactory L-ORAC measurements; the intermediate precision relative standard deviations (RSDint) ranged from 7.0 to 16.7%, the reproducibility relative standard deviations (RSDR) ranged from 14.8 to 19.4%, and the HorRat values ranged from 1.35 to 1.78.

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“…H-ORAC values of the extract from BR grains before and after boiling were measured according to the method of Watanabe J. et al, (2012). In addition, changes in AC content were measured by the pH differential method mentioned above, and changes in the composition of phenolic compounds were determined by HPLC.…”

Section: Changes In Antioxidant Activity and Composition Of Phenolic mentioning

confidence: 99%

Effects of Black Rice Containing Anthocyanins on Plasma and Hepatic Parameters in Type 2 Diabetic <i>db/db</i> Mice

Watanabe

2016

FSTR

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Method Validation by Interlaboratory Studies of Improved Hydrophilic Oxygen Radical Absorbance Capacity Methods for the Determination of Antioxidant Capacities of Antioxidant Solutions and Food Extracts

Cited by 90 publications

References 15 publications

Preliminary study on antioxidant properties, phenolic contents, and effects of Aesculus hippocastanum (horse chestnut) seed shell extract on in vitro cyclobutane pyrimidine dimer repair

Preliminary study on antioxidant properties, phenolic contents, and effects of Aesculus hippocastanum (horse chestnut) seed shell extract on in vitro cyclobutane pyrimidine dimer repair

Improvement and Interlaboratory Validation of the Lipophilic Oxygen Radical Absorbance Capacity: Determination of Antioxidant Capacities of Lipophilic Antioxidant Solutions and Food Extracts

Effects of Black Rice Containing Anthocyanins on Plasma and Hepatic Parameters in Type 2 Diabetic <i>db/db</i> Mice

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