Sometimes even identified -albeit incorrectly -with biomechanics, kinesiology is a field relying heavily on biomechanical methodology. Borellian Rennaisance approach, enhanced in the past with seminal contributions by scientists such as Marey, Braune, and Fischer, followed further by the work of the Berkeley Group, and later by a number of modern authors, has put classical mechanics in the centre of a paradigm taken to understand, analyse and quantitatively assess human movement. As this framework sets both a geometrical and a dynamical definition of the spatial (three dimensional -3D) movement of human body as a whole, an important further focus of the study may be directed to skeletal muscle itself, a basic actuator of movement and genuine biological system designed to produce mechanical force and cause movement. In this context, to monitor and evaluate human movement, we have a unique, second to none, method: electromyography (EMG); i.e. the recording of electrical activity of skeletal musculature. When studying kinesiological tasks, in particular, surface electromyography (sEMG) is the method's variant of choice. To quote Hess (Hess, 1954, as cited in Waterland, 1968): "The course of a movement is nothing else but a projection to the outside of a pattern of excitation taking place at a corresponding setting in the central nervous system". This thought reflects the importance of EMG signals as certain "windows" into the action of the central nervous system during the performance of a motor task. Kinesiological electromyography is, therefore, an established subfield of modern locomotion biomechanics. We witness today a number of professional journals, conferences, organizations and university-level courses devoted to this subject around the world. At the University of Zagreb, in particular, courses of this kind are spread across several departments (Medved, 2007). At the intersection of physiology and biomechanics, and with strong quantitative aspect, this discipline significantly contributes to our understanding of human movement and is therefore used in a number of basic and applied fields. Consequently, due to its inter-disciplinary nature, it is used by different professionals; physical therapists, medical doctors of various specialties, electrical and biomedical engineers, kinesiologists, to name but a few. To set the global framework, this chapter first shortly refers to the basics of methodology in biomechanical approach to human movement and of musculo-skeletal modelling. The method of surface electromyography is described next in some detail. Time domain signal processing methods are then presented, followed by the methods performed in the www.intechopen.com Biomechanics in Applications 350 frequency domain; all with the vision towards applications in the field of kinesiology. Chapter concludes by pointing to modern engineering solutions for multichannel sEMG.