Method To Estimate Growth of
            <i>Trichoderma reesei</i>
            and
            <i>Aspergillus wentii</i>
            in Mixed Culture on Cellulosic Substrates

Panda, T.; Bisaria, Virendra S.; Ghose, T. K.

doi:10.1128/aem.55.4.1044-1046.1989

Cited by 4 publications

(2 citation statements)

References 17 publications

(12 reference statements)

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“…Trichoderma reesei merupakan salah satu jenis kapang yang banyak diteliti terutama karena kemampuannya menghasilkan enzim selulase seperti endoglukanase dan selobiohidrolase tetapi sedikit memproduksi selobiase (Panda et al,. 1989;Juhasz et al, 2003;Pakula et al 2000;Pakula et al, 2003;Pakula et al, 2005).…”

Section: Pendahuluanunclassified

“…1989;Juhasz et al, 2003;Pakula et al 2000;Pakula et al, 2003;Pakula et al, 2005). Aspergillus niger memiliki kemampuan lebih, dalam menghasilkan enzim selobiase atau â-glukosidase dibanding T.reesei (Panda et al, 1989, Saha 1991) di samping itu kapang ini juga menghasilkan banyak macam enzim ekstraseluler, seperti -amilase, glukoamilase, dan glukosidase serta selulase (Saha 1991), oleh karena itu sering digunakan dalam pengolahan pangan untuk pengkayaan protein dari bahan berpati (Moo-Young 1983).…”

Section: Pendahuluanunclassified

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Penerapan Kultur Campuran Trichoderma reesei dan Aspergillus niger dalam Meningkatkan Kualitas Putak sebagai Pakan (APPLIED MIXED-CULTURED ASPERGILLUS NIGER AND TRICHODERMA REESEI TO INCREASE PUTAK QUALITY AS FEED)

Hilakore

Suryahadi

Wiryawan

et al. 2019

JVet

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Putak is a local feed in west Timor, East Nusa Tenggara Province. It is obtained from soft core of palm tree which is called gebang tree (Corypha gebanga). Putak contains high carbohydrate and fiber but low in protein. As an effort to increase the ptotein level in putak a research was conducted by fermenting it with mixed-cultured of Trichoderma reesei and Aspergillus niger. A laboratorium experimental method was used and arranged factorially in Completely Randomized Design 3 x 4 x 3 namely three inoculant levels of T. reesei (T): 5,0; 7,5 and 10,0 % (w/w), and four levels of A. niger (A) 0,5; 1,0; 1,5 and 2,0% (w/w). Mixed-cultured was done by: first, T.reesei as treatment mix with putak and incubation for two days, after that mix with A.niger, incubation for two days. The best result of this study was on combination of T.reesei 7.5% level and 1,5% of A.niger were crude protein (23.62%) and soluble protein (14.92%) was highest and fiber 10,17%.

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Section: Pendahuluanunclassified

Penerapan Kultur Campuran Trichoderma reesei dan Aspergillus niger dalam Meningkatkan Kualitas Putak sebagai Pakan (APPLIED MIXED-CULTURED ASPERGILLUS NIGER AND TRICHODERMA REESEI TO INCREASE PUTAK QUALITY AS FEED)

Hilakore

Suryahadi

Wiryawan

et al. 2019

JVet

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Microbial Protein Production by Submerged Fermentation of Mixed Cellulolytic Cultures

Zabala

Ferrer

Ledesma

et al. 1994

Advances in Bioprocess Engineering

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A simplified diphenylamine colorimetric method for growth quantification

Zhao

Xiang

Dai

et al. 2013

Appl Microbiol Biotechnol

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Cell growth needs to be monitored in biological studies and bioprocess optimization. In special circumstances, such as microbial fermentations in media containing insoluble particles, accurate cell growth quantification is a challenge with current methods. Only the Burton method is applicable in such circumstances. The original Burton method was previously simplified by adopting a two-step sample pretreatment in perchloric acid procedure to eliminate the need for DNA extraction. Here, we further simplified the Burton method by replacing the previous two-step perchloric acid pretreatment with a new and one-step diphenylamine reagent pretreatment. The reliability and accuracy of this simplified method were assessed by measuring the biomass of four model microorganisms: Escherichia coli, Streptomyces clavuligerus, Saccharomyces cerevisiae, and Trichoderma reesei grown in normal media or those containing solid particles. The results demonstrate that this new simplified method performs comparably to the conventional methods, such as OD600 or the previously modified Burton method, and is much more sensitive than the dry weight method. Overall, the new method is simple, reliable, easy to perform, and generally applicable in most circumstances, and it reduces the operation time from more than 12 h (for the previously simplified Burton method) to about 2 h.

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Method To Estimate Growth of Trichoderma reesei and Aspergillus wentii in Mixed Culture on Cellulosic Substrates

Abstract: A simple differential method based on measurement of an intracellular pigment of Aspergillus wentii was developed for estimation of the individual growths of two fungi, Trichoderma reesei and A. wentii, in mixed-culture fermentation of an insoluble cellulosic substrate.

Cited by 4 publications

References 17 publications

Penerapan Kultur Campuran Trichoderma reesei dan Aspergillus niger dalam Meningkatkan Kualitas Putak sebagai Pakan (APPLIED MIXED-CULTURED ASPERGILLUS NIGER AND TRICHODERMA REESEI TO INCREASE PUTAK QUALITY AS FEED)

Penerapan Kultur Campuran Trichoderma reesei dan Aspergillus niger dalam Meningkatkan Kualitas Putak sebagai Pakan (APPLIED MIXED-CULTURED ASPERGILLUS NIGER AND TRICHODERMA REESEI TO INCREASE PUTAK QUALITY AS FEED)

Microbial Protein Production by Submerged Fermentation of Mixed Cellulolytic Cultures

A simplified diphenylamine colorimetric method for growth quantification

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