Method for the quantitative analysis of cilostazol and its metabolites in human plasma using LC/MS/MS

Development and validation of a liquid chromatography/tandem mass spectrometry assay for the simultaneous determination of nateglinide, cilostazol and its active metabolite 3,4-dehydro-cilostazol in Wistar rat plasma and its application to pharmacokinetic study

“…Previous methods describing the determination of cilostazol and its metabolites involved liquid-liquid partitioning followed by solid phase extraction (SPE) using LC-MS/MS [11]. Other methods using HPLC-UV were involved with various gradient and isocratic conditions comprised of multiple steps of LLE or SPE procedures for estimating CLZ and its metabolites [10,[12][13][14][15].…”

“…There are more prescriptions of using NTG and CLZ in combination in diabetics and no such information on the drug-drug interaction effect of these two drugs when given together. While investigating the analytical methods in this regard, we found several individual methods for estimating these drugs alone in biological fluids by reverse phase chromatographic methods [11][12][13][14][15][16][17][18][19][20]. All these individual methods suffer from long analysis time, low sensitivity and difficult extraction procedures.…”

Formulation of Solid Dosage Form Containing Clopidogrel and Cilostazol and Its HPLC Analysis

“…Literature survey reveals several spectrophotometric and chromatographic [5][6][7][8][9][10][11][12][13][14][15][16][17][18] methods for the estimation of cilostazol and clopidogrel alone and in combination with other drugs. However, no HPLC method has been reported for the simultaneous determination of cilostazol and clopidogrel in combination dosage form.…”

RP-HPLC determination of cilostazol in human plasma: Application to pharmacokinetic study in male albino rabbit

“…Although cilostazol was determined in plasma, no pharmacokinetic parameters were determined [8][9][10]. The male albino rabbit (weight 1.3-1.5 kg) was selected for the study of pharmacokinetic parameters because of easy availability, easy withdrawal of amount of blood required, easy administration of drug, and its ease of handling.…”

“…It was determined in plasma [5][6][7][8][9][10][11][12], urine [13], tablet dosage form [14], and bulk drug [15][16]. The gaps in bioanalytical research were as follows: determination of analyte only [5][6][7], lacked adequate specificity from endogenous or xenobiotic interferences [6,8], no pharmacokinetic study [8][9][10], longer run time [6,11,12], and pharmacokinetic study in combination with other drug [13]. Cilostazol was also determined in tablet dosage form [14] and presence of its degradation products [15][16], which are unable to apply on pharmacokinetic study.…”