RP-HPLC determination of cilostazol in human plasma: Application to pharmacokinetic study in male albino rabbit

Abstract

Summary.A sensitive validated high-performance liquid-chromatographic method for analysis of cilostazol in human plasma (in vitro) has been developed, and it was applied to determine pharmacokinetics of cilostazol in male albino rabbit. Cilostazol was extracted from human plasma (in vitro) by acetonitrile, and efficient chromatographic elution was achieved on a C18 column (250 × 4.60 mm i.d., 0.5 µm particle size) with an isocratic mobile phase [acetonitrile-50 mM acetate buffer (pH 5.0, glacial acetic acid)-water (50:20:30)] at flow rate of 1.5 mL min −1 . Quantification was carried out by photo-diode array (PDA) detection at 248 nm. The linearity of the method was excellent over the range 0.2-2 μg mL −1 with low limits of detection (0.005 μg mL −1 ) and quantification (0.05 μg mL −1 ). The extraction recovery of the drug from plasma was consistently good (73.45-78.64%), with low relative standard deviation (0.44-1.65%). Robustness studies confirmed that peak area was unaffected by small changes in temperature, mobile phase (composition and pH). The maximum concentration (C max ) in rabbit (in vivo) was determined 1.620 μg mL −1 at t max (0.51 h) with 0.63% RSD by validated bioanalytical method.

Selective Methods for Cilostazol Assay in Presence of its Oxidative Degradation Product and Co Formulated Telmisartan Application to Tablet Formulation

“…CIL and TEL are official in USP [3], TEL is official in BP [4]. Literature survey yielded some published methods for CIL assay include: Spectrophotometry as single drug [5][6][7][8], as mixture with other drugs [9][10][11], HPLC methods as single drug [12][13][14][15], and with other drugs [16,17]. Several analytical methods were reported for Telmisartan assay include: Spectrophotometry as single drug [18][19][20], as mixture with other drugs [21][22][23][24][25][26][27], Spectrofluorimetric methods as a mixture with other drugs [28,29].…”