Method for quantitative determination of p‐hydroxybenzyl isothiocyanate in digests of seed meal of Sinapis alba L.

Abstract

Seed Meal of Sinapis alba L.were made with an E.E.L. flame photometer6 and recent work has shown that had they been made with a Unicam SP.900 flame spectrophotometer they would have been about 5 % lower.

Ionic Thiocyanate (SCN-) Production from 4-Hydroxybenzyl Glucosinolate Contained inSinapis albaSeed Meal

“…The half-life of 4-hydroxybenzyl isothiocyanate at pH 6.5 was the shortest at 6 min, increasing to 16,49,100,195,270,312, and 321 min with decreasing pH values of 6.0, 5.5, 5.0, 4.5, 4.0, 3.5, and 3.0, respectively (Figure 3). The hydrolytic instability of 4-hydroxybenzyl isothiocyanate, especially at higher pH values, explains its low extractability in unbuffered extracts of seed meal that had a pH of 5.3 and a sampling time of 48 h. Our data also confirm previous investigations indicating that alkaline pHs facilitate hydrolysis (7). However, we show that appreciable hydrolysis occurs at pH values as low as 3.0 and that in a soil environment buffered at pH values typically between 5 and 7, significant amounts of SCN -production are expected in a relatively short time period.…”

The effect of radio frequency heat treatment on nutritional and colloid-chemical properties of different white mustard (Sinapis alba L.) varieties

“…The glucosinolate content was determined by liberated glucose and measured by GOD-PERID reagent (Megazyme, Ireland). The sharp flavour causing breakdown compound, the p-hydroxy-benzyl isothiocyanate, was measured after autolysis or enzymic hydrolysis (Joseffson, 1968 Mineral content was measured by atomic absorption spectrophotometric method.…”

EFFECTS OF DIETARY CABBAGE (BRASSICA OLERACEA L.) ON THE TUMORIGENICITY OF 1, 2‐DIMETHYLHYDRAZINE IN MICE

“…cabbage samples and was reported as 3-methylindolyl isothiocyanate (Josefsson 1968). The myrosinase enzyme preparation required for the determination was made from yellow mustard seed (McCormick Company, Inc., Baltimore, Maryland) according to Appelqvist and Josefsson (1967).…”

“…Naturally occurring inducers of MFO activity are present in plants, particularly those belonging t o the cruciferae family. The inducers are produced during enzymatic hydrolysis of indolylmethyl glucosinolate by thioglucosidase (Gmelin and Virtanen 1961). These inducers are indole-3-acetonitrile, indole-3-carbinol, and 3, 3'-diindolylmethane, of which indole-3-carbinol produces the greatest enzyme elevation (Loub et al 1975).…”