Method for automated extraction and purification of nucleic acids and its implementation in microfluidic system

Mamaev, D. D.; Khodakov, Dmitriy A.; Dementieva, Ekaterina; Filatov, I. V.; Yurasov, D. A.; Cherepanov, A. I.; Vasiliskov, V. A.; Smoldovskaya, Olga; Zimenkov, Danila; Gryadunov, Dmitry; Mikhailovich, Vladimir; Заседателев, А. С.

doi:10.1134/s0003683811020128

Cited by 6 publications

(5 citation statements)

References 21 publications

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“…3.1 Sample processing and NA isolation procedure in disposable fluidic modules In this work, the protocol of sample preparation and NA extraction described earlier (Khodakov et al 2010;Mamaev et al 2011) was used as a basis for the development of the platform with multiple fluidic modules for automated NA isolation from several biological samples. The process involved two-step enzymatic and chemical lysis followed by SPE.…”

Section: Resultsmentioning

confidence: 99%

“…In addition, the purified NAs (5 μl) were used as a target for real-time PCR amplification of the B. thuringiensis gyrB gene fragment. The real-time PCR was performed as described previously (Mamaev et al 2011) using a 'DTlite' thermocycler (DNATechnology, LLC, Russia).…”

Section: Reference Specimens Clinical Samples Nucleic Acids Isolatimentioning

confidence: 99%

“…Previously, we reported on the development of the device for the isolation and purification of nucleic acids in an automated mode using an original microfluidic cartridge (Khodakov et al 2010;Mamaev et al 2011). The device allowed the isolation of nucleic acids only from a single biological sample using a combined enzymatic and chemical lysis step followed by the SPE.…”

Section: Introductionmentioning

confidence: 99%

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Rotary-based platform with disposable fluidic modules for automated isolation of nucleic acids

Mamaev

Shaskolskiy

Dementieva

et al. 2015

Biomed Microdevices

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We describe the development and evaluation of a rotary-based platform with multiple disposable fluidic modules for simultaneous automatic nucleic acid (NA) isolation from up to 24 biological samples. The procedure is performed inside insulated individual disposable modules, which minimizes both the risk of infection of personnel and laboratory cross-contamination. Each module is a segment of a circular cylinder containing a leak-proof inlet port for sample input, reservoirs with lyophilized chemicals and solvents, fluidic channels, stoppers, valves, a waste reservoir and an outlet port equipped with the standard micro test tube for NA collection. The entire platform, apart from the rotor that accommodates 24 modules, consists of functional elements that provide spinning of the rotor, reagent mixing, pressure delivery, and heating of reaction mixtures. The transfer of the reaction mixtures inside the modules is performed either with rotation of the rotor or with excessive air pressure applied to the module's reservoirs. The entire process takes less than 40 min, starting from the sample loading to the recovery of the purified NA, and it allows NA isolation both from bacterial cells and viral particles. The feasibility and reproducibility of the developed platform was demonstrated by the NA isolation from suspensions of Bacillus thuringiensis and Mycobacterium tuberculosis cells within a concentration range of 10(8) to 10(2) cells/ml. Isolation of NAs from blood plasma samples with varying concentration of hepatitis B and C viruses from 10(7) to 10(2) particles/ml were also successful. The purity and integrity of the extracted NAs were both reliable for performing quantitative PCR.

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Section: Resultsmentioning

confidence: 99%

Section: Reference Specimens Clinical Samples Nucleic Acids Isolatimentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Rotary-based platform with disposable fluidic modules for automated isolation of nucleic acids

Mamaev

Shaskolskiy

Dementieva

et al. 2015

Biomed Microdevices

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“…A key aspect of the above‐mentioned system consists in the saving of time, easy and reproducible sample manipulation and possible application of magnetic particles in automated systems for nucleic acid isolation . DNA isolation by magnetic particles is comparable to the traditional extraction procedures such as non‐magnetic silica and solvent procedures but with the added advantages of reduced solvent consumption and processing time.…”

Section: Resultsmentioning

confidence: 99%

SPE and purification of DNA using magnetic particles

Rittich

Španová

2013

J of Separation Science

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Superparamagnetic particles have been attractive for molecular diagnostics and analytical chemistry applications due to their unique magnetic properties and their ability to interact with various biomolecules of interest. This paper presents a critical overview of magnetic nano- and microparticles used as a solid phase for extraction and purification of DNAs. The mechanisms of DNA binding to the surface of functionalised magnetic particles are described. The most widely used materials including silica supports, organic polymers and other materials, mostly containing magnetite or paramagnetic metallic elements are reviewed. The main application areas of magnetic particles for DNA separation are briefly described.

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“…43,44 Based on recent research, a graphene-nanosheets/delta-MnO 2 nanocomposite can be used for disposing of interfering nickel ions from wastewater, 45 purication of DNA, molecular diagnostics and biochemistry. [46][47][48] Graphene-cobalt oxides in a composite lm with a graphene electrode are used for the electrochemical sensing of glucose 49,50 and H 2 O 2 . 51 Binary cobalt and manganese oxides are employed for electrochemical capacitor applications on activated carbon as advanced oxygen reduction electrocatalysts.…”

Section: Introductionmentioning

confidence: 99%

Fabrication of an (α-Mn₂O₃:Co)-decorated CNT highly sensitive screen printed electrode for the optimization and electrochemical determination of cyclobenzaprine hydrochloride using response surface methodology

et al. 2020

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Method for automated extraction and purification of nucleic acids and its implementation in microfluidic system

Cited by 6 publications

References 21 publications

Rotary-based platform with disposable fluidic modules for automated isolation of nucleic acids

Rotary-based platform with disposable fluidic modules for automated isolation of nucleic acids

SPE and purification of DNA using magnetic particles

Fabrication of an (α-Mn₂O₃:Co)-decorated CNT highly sensitive screen printed electrode for the optimization and electrochemical determination of cyclobenzaprine hydrochloride using response surface methodology

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Method for automated extraction and purification of nucleic acids and its implementation in microfluidic system

Cited by 6 publications

References 21 publications

Rotary-based platform with disposable fluidic modules for automated isolation of nucleic acids

Rotary-based platform with disposable fluidic modules for automated isolation of nucleic acids

SPE and purification of DNA using magnetic particles

Fabrication of an (α-Mn2O3:Co)-decorated CNT highly sensitive screen printed electrode for the optimization and electrochemical determination of cyclobenzaprine hydrochloride using response surface methodology

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Fabrication of an (α-Mn₂O₃:Co)-decorated CNT highly sensitive screen printed electrode for the optimization and electrochemical determination of cyclobenzaprine hydrochloride using response surface methodology