Method for Analysis of Nanoparticle Hemolytic Properties in Vitro

Dobrovolskaia, Marina A.; Clogston, Jeffrey D.; Neun, Barry; Hall, Jennifer B.; Patri, Anil K.; McNeil, Scott E.

doi:10.1021/nl0805615

Cited by 575 publications

(394 citation statements)

References 31 publications

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“…The UV-Vis spectra of the AgNPs band occur near 420 nm is shown in (Fig.2) indicating the formation of AgNPs due to reduction of silver ions by active molecules present in the seed extract. In accordance with previous literature studies were also reported by many researchers 17,18 . The brown color confirms that it was due to the reduction of Ag + which indicates the formation of AgNPs.…”

Section: Visual Observation and Uv-vis Spectroscopysupporting

confidence: 93%

Novel Biogenic Synthesis of Agnps From Seed Extract of Eugenia Uniflora L.: In Vitro Assessment of Their Antioxidant, Antimicrobial and Cytotoxic Potential

Dugganaboyana¹,

Sharanya²,

Kumar³

et al. 2017

Int. Res. J. Pharm.

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Here, we explored the medicinal uses of the novel biogenic silver nanoparticles of Eugenia uniflora L. (E. uniflora) seed extract as a cost effective, eco-friendly, reducing and stabilizing compounds. This study describes the synthesis of silver nanoparticles from Eugenia uniflora L. (E. uniflora) seed extract and their antioxidant, antibacterial and cytotoxic potential. Biosynthesis of AgNPs was monitored by UV-visible spectroscopy which revealed intense surface plasmon resonance bands at 447 nm and X-ray diffraction were employed to identify various functional groups and crystalline nature of AgNPs. Scanning electron microscopy studies demonstrated that synthesized particles were crystalline in nature with average size of 78-100nm. In vitro antioxidant effects were analyzed by butylated hydroxytoluene (BHT)) and 2,2-diphenyl-1-picrylhydrazyl (DPPH), which exhibited antioxidant activity there in the particles could scavenges the stable free radical DPPH of 75% to that o positive control BHT. The value of 50% inhibition concentration (IC50) of Standard BHT is 65.55 and E. uniflora is 38.63µg/ml. The antibacterial activity of green AgNPs displayed better zone of inhibition against selected human pathogens. The present study also investigated the toxicity effect of biogenic AgNPs against human prostate cancer cells (PC-3) and the inhibitory concentrations (IC50) were found to be 6.25μg/ml, respectively. It could be concluded that E. uniflora seed extract AgNPs can be used efficiently for potential antioxidant, antibacterial and cytotoxic potential AgNPs with potent biomedical applications.

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Section: Visual Observation and Uv-vis Spectroscopysupporting

confidence: 93%

Novel Biogenic Synthesis of Agnps From Seed Extract of Eugenia Uniflora L.: In Vitro Assessment of Their Antioxidant, Antimicrobial and Cytotoxic Potential

Dugganaboyana¹,

Sharanya²,

Kumar³

et al. 2017

Int. Res. J. Pharm.

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“…However, some particle interference due to haemoglobin precipitates adsorbed with the particles 321 on centrifugation has been reported, yielding a false negative result (Dobrovolskaia et al, 2008). 322…”

mentioning

confidence: 99%

Mixed protein–DNA gel particles for DNA delivery: Role of protein composition and preparation method on biocompatibility

Morán

Nogueira

Vinardell

et al. 2013

International Journal of Pharmaceutics

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“…Hemolysis of 510% and no change in globule size (p40.05) over 6 h in the serum stability study confirmed safety of the MEs for intravenous administration (Dobrovolskaia et al, 2008).…”

Section: Hemolysis and Serum Stabilitymentioning

confidence: 62%

Docosahexaenoic acid–mediated, targeted and sustained brain delivery of curcumin microemulsion

Shinde

Devarajan

2017

Drug Delivery

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We disclose microemulsions (ME) of curcumin (CUR) with docosahexaenoic acid (DHA)-rich oil (CUR DHA ME) for targeted delivery to the brain. MEs of CUR (5 mg/mL) with and without DHArich oil (CUR Capmul ME) suitable for intravenous and intranasal administration exhibited negative zeta potential, globule size 520 nm and good stability. Following intravenous delivery MEs exhibited high brain concentration with CUR DHA ME exhibiting a 2.8-fold higher C max than CUR solution. Furthermore, high and sustained concentration was demonstrated even at 24 h, which was 8-and 2-fold higher than CUR solution and CUR Capmul ME, respectively. Brain concentrations following intranasal administration were, however, substantially higher as evident from higher C max and AUC and sustained compared to corresponding intravenous formulations signifying nose to brain targeting. The high brain concentration of CUR DHA ME is ascribed to the targeting efficiency enabled by DHA-mediated transport across the blood-brain barrier (BBB). Histopathological and nasal toxicity confirmed safety of the MEs. Concentrationdependent cytotoxicity in vitro, on human glioblastoma U-87MG cell line was observed with CUR DHA MEs and with the blank DHA ME, implying anticancer potential of DHA. The dramatically low IC 50 value of CUR DHA ME (3.755 ± 0.24 ng/mL) is therefore attributed to the synergistic effect of CUR and DHA in the ME. The CUR concentration achieved with CUR DHA ME at 24 h which translated to 466-fold(intranasal) and 421-fold (intravenous) the IC 50 value in the U-87MG cell line suggests great promise of CUR DHA ME for therapy of brain cancer by both routes.

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Method for Analysis of Nanoparticle Hemolytic Properties in Vitro

Cited by 575 publications

References 31 publications

Novel Biogenic Synthesis of Agnps From Seed Extract of Eugenia Uniflora L.: In Vitro Assessment of Their Antioxidant, Antimicrobial and Cytotoxic Potential

Novel Biogenic Synthesis of Agnps From Seed Extract of Eugenia Uniflora L.: In Vitro Assessment of Their Antioxidant, Antimicrobial and Cytotoxic Potential

Mixed protein–DNA gel particles for DNA delivery: Role of protein composition and preparation method on biocompatibility

Docosahexaenoic acid–mediated, targeted and sustained brain delivery of curcumin microemulsion

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