Method development for genomic Legionella pneumophila DNA quantification by inductively coupled plasma mass spectrometry

Leclerc, Olga; Fraisse, P.O.; Labarraque, Guillaume; Oster, Caroline; Pichaut, Jean-Philippe; Baume, Maud; Jarraud, Sophie; Fisicaro, Paola; Vaslin-Reimann, Sophie

doi:10.1016/j.ab.2012.12.023

Cited by 8 publications

(8 citation statements)

References 34 publications

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“…393 The authors did overcome interferences from 15 N 16 O 1 and 14 N 16 O 1 H 1 and achieved a detection limit for phosphorus in DNA of 0.1 ng g À1 . They further claimed that the accuracy of the method is much better than that of quantitative polymerase chain reaction (qPCR).…”

Section: Element-specific Detection Of Small Molecules In Biological mentioning

confidence: 99%

Inductively Coupled Plasma Mass Spectrometry

Jakubowski

Horsky

Roos

et al. 2014

Sector Field Mass Spectrometry for Elemental and Isotopic Analysis

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ICP-MS is based on the formation of (preferentially monovalent positively) charged atomic ions in an inductively coupled Ar plasma at almost 10 000 K. The ions formed are transferred from the plasma source at ambient pressure into a mass separator operated at high vacuum via a set of cones. The ions are separated according to their mass/charge ratio in the mass separator (quadrupole, magnetic sector field or time-of-flight mass separator). In most cases, the ions are detected using a secondary electron multiplier; in some set-ups (also) a Faraday cup can be used. Single-collector (scanning mass spectrometer usually used for quantitative elemental analysis) or multicollector (static operation of mass spectrometer for precise isotope ratio analysis) configurations can be found (Figures 12.1 and 12.2).

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Section: Element-specific Detection Of Small Molecules In Biological mentioning

confidence: 99%

Inductively Coupled Plasma Mass Spectrometry

Jakubowski

Horsky

Roos

et al. 2014

Sector Field Mass Spectrometry for Elemental and Isotopic Analysis

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“…Some other physicochemical methods have high sensitivity, accuracy and clear metrology traceability; however, they are still often hampered by the efficiency of the phosphodiesterase enzyme digestion [ 104 , 105 ]. HR-ICPMS has higher sensitivity and specificity, which can accurately analyse the mass fraction of phosphorus, that stoichiometrically presents in DNA molecule and consequently achieve the DNA concentration with high precision and clear traceability [ 106 ].…”

Section: Analytical Approachesmentioning

confidence: 99%

mRNA Therapeutic Modalities Design, Formulation and Manufacturing under Pharma 4.0 Principles

et al. 2021

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In the quest for a formidable weapon against the SARS-CoV-2 pandemic, mRNA therapeutics have stolen the spotlight. mRNA vaccines are a prime example of the benefits of mRNA approaches towards a broad array of clinical entities and druggable targets. Amongst these benefits is the rapid cycle “from design to production” of an mRNA product compared to their peptide counterparts, the mutability of the production line should another target be chosen, the side-stepping of safety issues posed by DNA therapeutics being permanently integrated into the transfected cell’s genome and the controlled precision over the translated peptides. Furthermore, mRNA applications are versatile: apart from vaccines it can be used as a replacement therapy, even to create chimeric antigen receptor T-cells or reprogram somatic cells. Still, the sudden global demand for mRNA has highlighted the shortcomings in its industrial production as well as its formulation, efficacy and applicability. Continuous, smart mRNA manufacturing 4.0 technologies have been recently proposed to address such challenges. In this work, we examine the lab and upscaled production of mRNA therapeutics, the mRNA modifications proposed that increase its efficacy and lower its immunogenicity, the vectors available for delivery and the stability considerations concerning long-term storage.

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“…Thus the extended capability for the determination of both P and S determination opens up important biological molecules for high accuracy measurement. Leclerc et al 214 have developed a method for the quantitation of Legionella pneumophila genomic DNA. The method is based on the use of SF-ICP-MS to measure the mass fraction of 31 P, stoichiometrically present in the DNA molecules.…”

Section: Macromolecular Analysismentioning

confidence: 99%