Method development for determining the iohexol in human serum by micellar electrokinetic capillary chromatography

Development and validation of an HPLC-UV method for determination of iohexol in human plasma

“…Capillary electrophoresis [11,12] and inductively coupled plasmaatomic emission spectroscopy [13] have also been employed for determination of iohexol in biological samples. However, because of insufficient details in the methodology or lack of rigorous validations in some of the published HPLC-UV methods, we could not reproduce the results of these papers.…”

Serum iohexol analysis by micellar electrokinetic capillary chromatography

“…To further improve the precision indirectly we elected to inject after every ten samples a standard. Kitahashi and Furuta [20] measured recently iohexol in serum also by CE. In spite of long multiple wash steps (15 min vs. 80 s in this work) a higher RSD resulted; and in spite of the high voltages (25 vs. 8 kV in this work) used, the migration time was twice as long as in the present method.…”

Development of a capillary electrophoretic method for determination of plasma clearance of iohexol in dogs and cats

“…1), it was expected that iohexol would be complexated to a higher degree than iopromide, which would result in a higher negative effective mobility. Borate buffer was used in the previously published CE methods (Jenkins and Ratnaike, 2000;Rocco et al, 1996;Shihabi and Constantinescu, 1992) and both borate and SDS were applied in the micellar electro-kinetic chromatography methods (Kitahashi and Furuta, 2004;Shihabi and Hinsdale, 2006). In order to keep the method as simple as possible, plain borate buffers without micellar agents were chosen for the presented work.…”

“…The most commonly used technique for the determination of iohexol in plasma/serum is high-performance liquid chromatography (HPLC) in conjunction with ultraviolet (UV; De Baere et al, 2012;Edelson et al, 1983) or mass spectrometric (MS) detection (Annesley and Clayton, 2009;Lee et al, 2006), but capillary zone electrophoresis (Jenkins et al, 2000;Rocco et al, 1996;Shihabi and Constantinescu, 1992) and micellar electro-kinetic chromatography (Kitahashi and Furuta, 2004;Shihabi and Hinsdale, 2006) have also been utilized. The HPLC-UV and HPLC-MS methods cover a concentration range from 0.5 to 1500 mg/L (Lee et al, 2006), which gives a lower limit of quantification but also a higher upper limit of quantification than that described for the capillary electrophoresis (CE) based methods (0-1000, 8-260 mg/L; Kitahashi and Furuta, 2004;Shihabi and Hinsdale, 2006). The analytical range needed may vary in clinical and research situations, but according to the authors' experience, a detection range between 15 and 3000 mg/L is necessary to cover most situations where iohexol analysis for GFR determination is performed.…”