Development and validation of an HPLC-UV method for determination of iohexol in human plasma

Soman, Rohit; Zahir, Hamim; Akhlaghi, Fatemeh

doi:10.1016/j.jchromb.2004.11.046

Cited by 43 publications

(40 citation statements)

References 17 publications

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“…The plasma was aliquoted after a centrifugation at 5,976 g for 2 min and stored at -20°C until analysis. The serum iohexol concentration was measured by HPLC as previously described [32]. The plasma clearance of iohexol was calculated from the dose and the area under the curve, using a bi-compartmental model with GraphPad Prism (GraphPad software, La Jolla, CA, USA) and was expressed in mL/min/kg.…”

Section: Methodsmentioning

confidence: 99%

Adenine Rich Diet Is Not a Surrogate of 5/6 Nephrectomy in Rabbits

Florens

Lemoine

Pelletier

et al. 2017

Nephron

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Background: Animal models are important tools needed to understand the mechanisms underlying the progression of renal disease and to implement new therapeutic approaches. A non-surgical model of chronic kidney disease (CKD) developed by chemical nephrectomy using an adenine-enriched diet has been shown to be a robust model to induce kidney failure in mice and rats. The purpose of this study was to implement an adenine diet to induce CKD in rabbits. Methods: Male New Zealand rabbits were fed for 4 weeks with a diet containing 0.75% (w/w) adenine, and renal function was assessed by measuring plasma urea and creatinine concentrations. The glomerular filtration rate (GFR) was measured using the plasmatic clearance of Iohexol. Kidney histology was performed with haematoxylin erythrosine saffron and Sirius red staining. Results: In contrast to what was observed in rodents, adenine diet failed to induce kidney failure in rabbits as is evident in the plasma concentrations of creatinine and urea and the direct measurement of GFR or histopathological studies. Conclusion: Adenine diet is not a surrogate of subtotal nephrectomy to induce kidney failure in rabbits. Several interspecies differences in metabolism and renal physiology could account for this observation.

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Section: Methodsmentioning

confidence: 99%

Adenine Rich Diet Is Not a Surrogate of 5/6 Nephrectomy in Rabbits

Florens

Lemoine

Pelletier

et al. 2017

Nephron

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“…mGFR by iohexol clearance (19 -21) was determined during the first three days of admission using fasting plasma sampling and HPLC (20,21). The same samples were used to measure creatinine levels by the Roche Lab "compensated" IDMS-traceable method (Hitachi Modular P-800 Roche Diagnostics, Germany) and CysC by particle enhanced immunonephelometry on a BN II system (Dade Behring Marburg GMBH, USA).…”

Section: Gfr Measurement and Estimationmentioning

confidence: 99%

Assessing Glomerular Filtration Rate in Hospitalized Patients

Segarra¹,

Torre²,

Ramos³

et al. 2011

Clinical Journal of the American Society of Nephrology

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SummaryBackground and objectives A specific method is required for estimating glomerular filtration rate GFR in hospitalized patients. Our objective was to validate the Chronic Kidney Disease Epidemiology Collaboration (CKD-EPI) equation and four cystatin C (CysC)-based equations in this setting.Design, setting, participants, & measurements This was an epidemiologic, cross-sectional study in a random sample of hospitalized patients (n ϭ 3114). We studied the accuracy of the CKD-EPI and four CysC-based equations-based on (1) CysC alone or (2) adjusted by gender; (3) age, gender, and race; and (4) age, gender, race, and creatinine, respectively-compared with GFR measured by iohexol clearance (mGFR). Clinical, biochemical, and nutritional data were also collected.Results The CysC equation 3 significantly overestimated the GFR (bias of 7.4 ml/min per 1.73 m 2 ). Most of the error in creatinine-based equations was attributable to calculated muscle mass, which depended on patient's nutritional status. In patients without malnutrition or reduced body surface area, the CKD-EPI equation adequately estimated GFR. Equations based on CysC gave more precise mGFR estimates when malnutrition, extensive reduction of body surface area, or loss of muscle mass were present (biases of 1 and 1.3 ml/min per 1.73 m 2 for equations 2 and 4, respectively, versus 5.9 ml/min per 1.73 m 2 for CKD-EPI). ConclusionsThese results suggest that the use of equations based on CysC and gender, or CysC, age, gender, and race, is more appropriate in hospitalized patients to estimate GFR, since these equations are much less dependent on patient's nutritional status or muscle mass than the CKD-EPI equation.

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“…13 The concentration of free MPA was assessed using ultrafiltration followed by liquid chromatography/mass spectrometry/mass spectrometry in plasma. 14 Concentrations of iohexol 15 and acetaminophen 6 were measured by HPLC-UV. All methods were developed in-house and validated according to the guidelines set by the U.S. Food and Drug Administration.…”

Section: Determination Of Drug Concentrationsmentioning

confidence: 99%

Effect of Diabetes Mellitus on Mycophenolate Sodium Pharmacokinetics and Inosine Monophosphate Dehydrogenase Activity in Stable Kidney Transplant Recipients

Patel¹,

Richman²,

Yang³

et al. 2007

Therapeutic Drug Monitoring

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Effect of diabetes mellitus on mycophenolic acid (MPA) pharmacokinetics and catalytic activity of inosine monophosphate dehydrogenase (IMPDH) was investigated in maintenance kidney transplant recipients. Demographically matched diabetic (n = 9) and nondiabetic (n = 9) patients were included in a 12-hour open-label, steady-state study after oral administration of entericcoated mycophenolate sodium. Concentrations of total MPA and free MPA, MPA-glucuronide, and acyl-MPA-glucuronide were measured and oral acetaminophen absorption was used as a marker for gastric-emptying rate. Median (range) of MPA area under the curve 0-12 was 36.7 (range, 16.4-116.4) mg * h/L in diabetic and 48.2 (range, 34.9-80.1) mg * h/L in nondiabetic patients (P = 0.49). All other primary pharmacokinetic parameters, including time to maximum concentration, for total or unbound MPA as well as MPA metabolites were comparable. In contrast, IMPDH activity was 17.5 ± 2.8 versus 46.6 ± 2.5 nmol XMP/h/mg protein in diabetics and nondiabetics, respectively (P < 0.0001) and was significantly lower in the diabetics irrespective of concomitant therapy with cyclosporine or tacrolimus. This study demonstrated that diabetes does not alter MPA pharmacokinetics when administered as enteric-coated mycophenolate sodium; however, IMPDH activity appeared to be significantly lower in patients with diabetes independent of the unbound or total concentrations of MPA. Further investigations are warranted to investigate the regulation of IMPDH enzyme in patients with diabetes.

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Development and validation of an HPLC-UV method for determination of iohexol in human plasma

Cited by 43 publications

References 17 publications

Adenine Rich Diet Is Not a Surrogate of 5/6 Nephrectomy in Rabbits

Adenine Rich Diet Is Not a Surrogate of 5/6 Nephrectomy in Rabbits

Assessing Glomerular Filtration Rate in Hospitalized Patients

Effect of Diabetes Mellitus on Mycophenolate Sodium Pharmacokinetics and Inosine Monophosphate Dehydrogenase Activity in Stable Kidney Transplant Recipients

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