Method Development and Validation for Simultaneous Estimation of Omeprazole and Ofloxacin by using RP-HPLC

Akula, Ganesh; Rajashekar, Perusomula; Nirmala, Rangu; Kanakaiah, K.; Jaswanth, A.

doi:10.5958/2231-5675.2018.00028.5

Asian Jour. Pharmac. Anal.

2018

DOI: 10.5958/2231-5675.2018.00028.5

|View full text |Cite

Method Development and Validation for Simultaneous Estimation of Omeprazole and Ofloxacin by using RP-HPLC

Ganesh Akula¹,

Perusomula Rajashekar²,

Rangu Nirmala³

et al.

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...

Citation Types

Supporting

Mentioning

Contrasting

Year Published

2022

2023

Publication Types

Select...

Article3

Relationship

Self Cite0

Independent3

Authors

Journals

Cited by 3 publications

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

Fourier Transform Infrared Spectrophotometry: An Eco-friendly green tool for quantification of Omeprazole in Pharmaceutical formulation

Prajapati¹,

Chandarana²

2022

RJPT

View full text Add to dashboard Cite

An eco-friendly method for quantification of Omeprazole in Pharmaceutical solid dosage form has been developed using Fourier transform infrared spectrophotometry. The proposed method avoids use of solvents which are commonly used for other methods of quantification e.g. Liquid Chromatography, UV spectrophotometry etc. The developed method has been validated for quantification of Omeprazole in marketed formulation as per ICH Q2 R (1) guidelines. The method is based on Beer-lamberts’ law. For the quantification C-O stretch absorption band at 1204 cm−1 was selected which is typically in the range of 1300 – 1000cm-1 in IR spectrum. The linearity of Omeprazole was obtained in the concentration range of 5 – 30mg/g with R2 value of 0.999. The % recovery was calculated with Intra and Inter day precision study.

show abstract

Fourier Transform Infrared Spectrophotometry: An Eco-friendly green tool for quantification of Omeprazole in Pharmaceutical formulation

Prajapati¹,

Chandarana²

2022

RJPT

View full text Add to dashboard Cite

show abstract

Gastroprotective Activity of Aerial Leaves of Allium cepa

Laxmi,

Sulthana,

Shirisha

et al. 2023

AJRC

View full text Add to dashboard Cite

The present study was performed to evaluate the Antiulcer activity of Methanolic extract of Allium cepa leaves (MEAC) in Albino rats. Antiulcer activity of MEAC was investigated by using Ethanol induced gastric mucosal damage and Pylorous ligated gastric ulcers. Evaluation was done on both models comparing with reference standard Omeprazole (20mg/kg). The parameters taken to assess anti-ulcer for pylorus ligation method were volume gastric secretion, pH, free acidity, total acidity and ulcer index. In vivo studies were carried on the plant extract and results were found that protection of the Methanolic extract of Allium cepa leaves against characteristic lesions may be due to both reduction in gastric acid secretion and gastric cycloprotein and this may be due to the presence of Flavanoids. In this study we observed that, Methanolic extract of leaves of Allium cepa (MEAC) provides significant antiulcer activity against gastric ulcers in rats. The result of MEAC showed significant reduction in the ulcer index, when compared to control. The antiulcer activity extract may be attributed to their flavanoids content and antioxidant property. These findings indicate that MEAC display potential antiulcerogenic activity.

show abstract

A Review on Method Development of High Performance Liquid Chromatography (HPLC) and its Applications

Choudhari,

Rasve,

Khilare

et al. 2023

RJPP

View full text Add to dashboard Cite

The most common method for identifying, separating, and measuring the medication is HPLC.To improve the approach, a variety of chromatographic factors including sample pretreatment, mobile phase selection, column selection, and detector selection were examined. Reviewing the technique creation, optimization, and validation is the article's main goal. The chemistry of the molecules, the synthetic pathway, solubility, polarity, pH and pKa values, and the activity of the functional groups are all factors that affect the creation of an HPLC technique. Information on several stages and properties including accuracy, specificity, linearity, limit of detection, and limit of quantification are provided through the validation of HPLC methods in accordance with ICH Guidelines1.

show abstract

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

hi@scite.ai

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

Method Development and Validation for Simultaneous Estimation of Omeprazole and Ofloxacin by using RP-HPLC

Cited by 3 publications

References 0 publications

Fourier Transform Infrared Spectrophotometry: An Eco-friendly green tool for quantification of Omeprazole in Pharmaceutical formulation

Fourier Transform Infrared Spectrophotometry: An Eco-friendly green tool for quantification of Omeprazole in Pharmaceutical formulation

Gastroprotective Activity of Aerial Leaves of Allium cepa

A Review on Method Development of High Performance Liquid Chromatography (HPLC) and its Applications

Contact Info

Product

Resources

About