Methionine supply during the periparturient period enhances insulin signaling, amino acid transporters, and mechanistic target of rapamycin pathway proteins in adipose tissue of Holstein cows

Liang, Yusheng; Batistel, Fernanda; Parys, C.; Loor, Juan J.

doi:10.3168/jds.2018-15738

Cited by 26 publications

(38 citation statements)

References 60 publications

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“…The SLC3A2 gene is a member of the solute carrier family 3 member 2, which encodes a bidirectional antiporter that regulates the exchange of L-AA between the extracellular and intracellular compartments (Nicklin et al, 2009). Our recent work underscored that enhanced postruminal supply of Met led to greater SL-C3A2 mRNA abundance in adipose tissue of Holstein cows during the periparturient period (Liang et al, 2019). As in the present study, upregulation of SLC3A2 in cultures with incMet was also observed in MAC-T cells (Dong et al, 2018) and human muscle tissue with increased essential AA supplementation (Drummond et al, 2010).…”

Section: Discussionmentioning

confidence: 83%

Alterations in immune and antioxidant gene networks by gamma-d-glutamyl-meso-diaminopimelic acid in bovine mammary epithelial cells are attenuated by in vitro supply of methionine and arginine

Dai

Coleman

Lopes

et al. 2021

Journal of Dairy Science

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Section: Discussionmentioning

confidence: 83%

Alterations in immune and antioxidant gene networks by gamma-d-glutamyl-meso-diaminopimelic acid in bovine mammary epithelial cells are attenuated by in vitro supply of methionine and arginine

Dai

Coleman

Lopes

et al. 2021

Journal of Dairy Science

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“…acetyl-CoA and propionyl-CoA) decreased, but also there was a shift in post-hepatic AA utilization from adipose tissue (prepartum) to mammary gland for milk production (postpartum). Recent data underscored that protein abundance of branched chain ketoacid dehydrogenase kinase (BCKDK) did not change between pre and postpartum periods in adipose tissue of periparturient Holstein cows, suggesting it is a response that helps channel circulating BCAA to the mammary gland [49].…”

Section: Discussionmentioning

confidence: 99%

Prepartum dietary energy intake alters adipose tissue transcriptome profiles during the periparturient period in Holstein dairy cows

Minuti

Bionaz

Lopreiato

et al. 2020

J Animal Sci Biotechnol

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BackgroundThe aim of the study was to investigate the effect of energy overfeeding during the dry period on adipose tissue transcriptome profiles during the periparturient period in dairy cows.MethodsFourteen primiparous Holstein cows from a larger cohort receiving a higher-energy diet (1.62 Mcal of net energy for lactation/kg of dry matter; 15% crude protein) for ad libitum intake to supply 150% (OVR) or 100% (CTR) of energy requirements from dry off until parturition were used. After calving, all cows received the same lactation diet. Subcutaneous adipose tissue (SAT) biopsies were collected at − 14, 1, and 14 d from parturition (d) and used for transcriptome profiling using a bovine oligonucleotide microarray. Data mining of differentially expressed genes (DEG) between treatments and due to sampling time was performed using the Dynamic Impact Approach (DIA) and Ingenuity Pathway Analysis (IPA).ResultsThere was a strong effect of over-feeding energy on DEG with 2434 (False discovery rate-corrected P < 0.05) between OVR and CTR at − 14 d, and only 340 and 538 at 1 and 14 d. The most-impacted and activated pathways in the Kyoto Encyclopedia of Genes and Genomes (KEGG) database that were highlighted by DIA analysis at − 14 d in OVR vs. CTR included 9 associated with carbohydrate metabolism, with ‘Pyruvate metabolism’, ‘Glycolysis/gluconeogenesis’, and ‘Pentose phosphate pathway’ among the most-activated. Not surprisingly, OVR led to marked activation of lipid metabolism (e.g. ‘Fatty acid biosynthesis’ and ‘Glycerolipid metabolism’). Unexpected metabolic pathways that were activated at − 14 d in OVR included several related to metabolism of amino acids (e.g. branched chain) and of cofactors and vitamins (thiamin). Among endocrine and immune system pathways, at − 14 d OVR led to marked activation of ‘PPAR signalling’ and ‘Antigen processing and presentation’. Among key pathways affected over time in OVR, a number were related to translation (e.g. mTOR signaling), endocrine/immune signaling (CXCR4 and IGF1), and lipid metabolism (oxidative phosphorylation) with greater activation in OVR vs. CTR specifically at − 14 d. Although statistical differences for several pathways in OVR vs. CTR nearly disappeared at 1 and 14 vs. − 14 d, despite the well-known catabolic state of adipose depots after calving, the bioinformatics analyses suggested important roles for a number of signaling mechanisms at − 14 vs. 14 than 1 vs. -14 d. This was particularly evident in cows fed to meet predicted energy requirements during the dry period (CTR).ConclusionsData underscored a strong activation by overfeeding energy of anabolic processes in the SAT exclusively prepartum. The study confirmed that higher-energy diets prepartum drive a transcriptional cascade of events orchestrated in part by the activation of PPARγ that regulate preadipocyte differentiation and lipid storage in SAT. Novel aspects of SAT biology to energy overfeeding or change in physiologic state also were uncovered, including the role of amino acid metabolism, mTOR signalin...

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“…However, changes in AA transporters and insulin signaling indicated that insulin sensitivity of the mammary gland was enhanced with supplemental Met ( Ma et al, 2019 ). In subcutaneous adipose tissue, the mRNA and protein abundance of some AA transporters and p-MTOR were upregulated with enhanced Met supply, while changes in insulin signaling and plasma glucose also indicated that Met helped improve insulin sensitivity ( Liang et al, 2019b ). Thus, enhancing Met supply during the periparturient period may lead to MTOR activation in immune cells, as well as improved nutrient uptake, which could help to support proliferation and development.…”

Section: Aa Immune Function and Oxidative Stressmentioning

confidence: 99%

Amino acids and the regulation of oxidative stress and immune function in dairy cattle

Coleman

Lopreiato

Alharthi

et al. 2020

Journal of Animal Science

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Methionine supply during the periparturient period enhances insulin signaling, amino acid transporters, and mechanistic target of rapamycin pathway proteins in adipose tissue of Holstein cows

Cited by 26 publications

References 60 publications

Alterations in immune and antioxidant gene networks by gamma-d-glutamyl-meso-diaminopimelic acid in bovine mammary epithelial cells are attenuated by in vitro supply of methionine and arginine

Alterations in immune and antioxidant gene networks by gamma-d-glutamyl-meso-diaminopimelic acid in bovine mammary epithelial cells are attenuated by in vitro supply of methionine and arginine

Prepartum dietary energy intake alters adipose tissue transcriptome profiles during the periparturient period in Holstein dairy cows

Amino acids and the regulation of oxidative stress and immune function in dairy cattle

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