Methanosarcina horonobensis sp. nov., a methanogenic archaeon isolated from a deep subsurface Miocene formation

Abstract

A methanogenic organism, designated strain HB-1 T , from the domain Archaea was isolated from groundwater sampled from a subsurface Miocene formation located in Horonobe, Hokkaido, Japan. The strain grew on methanol, dimethylamine, trimethylamine, dimethylsulfide and acetate but not on monomethylamine, H 2 /CO 2 , formate, 2-propanol, 2-butanol or cyclopentanol. Cells were Gram-reaction-negative, non-motile, irregular cocci that were 1.4-2.9 mm in diameter and occurred singly or in pairs. The strain grew at 20-42 6C (optimum 37 6C), at pH 6.0-7.75 (optimum pH 7.0-7.25) and in 0-0.35 M NaCl (optimum 0.1 M). The G+C content of the genomic DNA was 41.4 mol%. 16S rRNA gene sequencing revealed that the strain was a member of the genus Methanosarcina but that it clearly differed from all recognized species of this genus (93.1-97.9 % sequence similarity). The phenotypic and phylogenetic features of strain HB-1 T indicate that it represents a novel species of the genus Methanosarcina, for which the name Methanosarcina horonobensis sp. nov. is proposed. The type strain is HB-1 T (5DSM 21571 T 5JCM 15518 T 5NBRC 102577 T ).

Methylotrophic methanogenesis governs the biogenic coal bed methane formation in Eastern Ordos Basin, China

“…The primary methanogenic archaea propagated on these enrichments were closely related to the cultured methanogens M. chelungpuianus and M. horonobensis (Table 3; Fig. 5), which are both methylotrophic methanogens (Shimizu et al 2011;Wu and Lai 2011). M. chelungpuianus was also the dominant archaea detected in the water samples (Fig.…”

“…4). This might be because that the temperature was not optimal for the production of methane, given that the temperature range for growth was 20 to 42°C for M. horonobensis (Shimizu et al 2011), 25 to 50°C for M. congolense (Cuzin et al 2001), and 24 to 45°C for M. chelungpuianus (Wu and Lai 2011).…”

“…The sequences of these bands were >98% identical to the SXLL-A01-derived sequence from the archaeal clone library from the water produced. SLG-A03 was closely related to the 16S rRNA gene from the cultured methanogen Methanosarcina horonobensis (94% identity), which grew on methanol, dimethylamine, trimethylamine, dimethylsulfide, and acetate (Shimizu et al 2011). Both SLG-A04 and SLG-A06 showed the closest identity to the 16S rRNA gene from the cultured ammonia-oxidizing archaeon Candidatus nitrosopumilus (91% identity) (Matsutani et al 2009).…”

Methanohalophilus levihalophilus sp. nov., a slightly halophilic, methylotrophic methanogen isolated from natural gas-bearing deep aquifers, and emended description of the genus Methanohalophilus

“…Within this order, the methylotrophic methanogens 'Methanococcoides vulcani' (L' Haridon et al, 2014), Methanolobus profundi (Mochimaru et al, 2009), Methanolobus zinderi (Doerfert et al, 2009), Methanosarcina baltica (von Klein et al, 2002), Methanosarcina horonobensis (Shimizu et al, 2011) and Methermicoccus shengliensis (Cheng et al, 2007) were originally isolated from deep subsurface environments such as marine sediments, formation waters from oil and gas reservoirs, and coal beds. It has been reported that methylotrophic methanogens are predominantly detected among archaea in coal seams (Dawson et al, 2012;Guo et al, 2012;Shimizu et al, 2007;Wawrik et al, 2012) and they have been suggested to be associated with the anaerobic degradation of coal to methane (Strąpoć et al, 2010).…”

Genomic and phenotypic differentiation among Methanosarcina mazei populations from Columbia River sediment

“…This putative operon was found in all members of mazei-WC, including M. mazei C16, but excluding M. mazei WWM610 and the two closest isolates, suggesting a recent loss in this subclade. This gene set was also found in a sparsely distributed set of Methanosarcina strains, namely M. horonobensis HB1 (Shimizu et al, 2011), M. sp. Naples 100 and M. calensis Cali.…”