Methanol Extract ofArtemisia apiaceaHance Attenuates the Expression of Inflammatory Mediators via NF-κB Inactivation

Abstract

Artemisia apiacea Hance is one of the most widely used herbs for the treatment of malaria, jaundice, and dyspeptic complaint in oriental medicine. This study investigated the effects of methanol extracts of A. apiacea Hance (MEAH) on the induction of inducible nitric oxide synthase (iNOS) and proinflammatory mediators by lipopolysaccharide (LPS) in Raw264.7 macrophage cells and also evaluated the in vivo effect of MEAH on carrageenan-induced paw edema in rats. MEAH treatment in Raw264.7 cells significantly decreased LPS-inducible nitric oxide production and the expression of iNOS in a concentration-dependent manner, while MEAH (up to 100 μg/mL) had no cytotoxic activity. Results from immunoblot analyses and ELISA revealed that MEAH significantly inhibited the expression of cyclooxygenase-2, tumor necrosis factor-α, interleukin-1β, and interleukin-6 in LPS-activated cells. As a plausible molecular mechanism, increased degradation and phosphorylation of inhibitory-κBα and nuclear factor-κB accumulation in the nucleus by LPS were partly blocked by MEAH treatment. Finally, MEAH treatment decreased the carrageenan-induced formation of paw edema and infiltration of inflammatory cells in rats. These results demonstrate that MEAH has an anti-inflammatory therapeutic potential that may result from the inhibition of nuclear factor-κB activation, subsequently decreasing the expression of proinflammatory mediators.

Water extract of Artemisia ordosica enhances antioxidant capability and immune response without affecting growth performance in weanling piglets

“…However, the other compounds in Artemisia ordosica , such as terpenoids, coumarins, acetylenes, caffeoylquinic acids and sterol, were not measured. In an effort to identify active constituents of methanol extract of Artemisia apiacea , it was analysed by GC‐MS (Ryu et al, ). Unfortunately, we did not detect the composition of WEAO by GC‐MS analysis.…”

“…Previous study reported that the antioxidant protection of apple, similar to that of many other dietary plants, may be due largely to the presence of phenolics (Eberhardt, Lee, & Liu, 2000;Tsao, Yang, JCY, & Zhu, 2003 TA B L E 5 Effects of WEAO on the contents of cytokines in serum of weanling piglets a and sterol, were not measured. In an effort to identify active constituents of methanol extract of Artemisia apiacea, it was analysed by GC-MS (Ryu et al, 2013). Unfortunately, we did not detect the composition of WEAO by GC-MS analysis.…”

Artemisia annua (Sweet Annie), Other Artemisia Species, Artemisinin, Artemisinin Derivatives, and Malaria

“…31 The closely related herb A. apiacea inhibited nuclear factor κB activation in rats, thus limiting inflammation. 32 In vitro, A. capillaris inhibited secretion of the pro-inflammatory cytokines tumor necrosis factor-α and IL-1. 33…”

“…In fact, the only adverse effect noted in the clinical trials on A. annua tea has been nausea which remits when therapy is completed. 30,32 Co-administration with common, mild anti-emetic herbs such as mint, ginger or other aromatic spices may alleviate or prevent nausea in some patients based on empirical evidence. The safety of A. annua in pregnancy has not been studied.…”

Anti-allergic effect of Artemisia extract in rats

“…A recently identified chemical substance in A. apiacea extract, isolated by the China Traditional Chinese Medicine Academy (http://www.catcm.ac.cn/), has shown a good antimalarial effect that is equal to artemisinin (5-7). A. apiacea is a herb commonly used in traditional Chinese medicine, which exhibits anti-inflammatory and antipyresis, antibacterial, antiparasitic and immunosuppressive effects (8). Therefore, the aim of the present study was to evaluate the effect of Artemisia extract on the allergic response induced by compound 48/80 in rats.…”