The present experiment was conducted to investigate the effects of water extract of Artemisia ordosica (WEAO) on growth performance, antioxidant capability and immune response in weanling piglets. Seventy‐two 28‐day‐old weanling piglets were randomly allocated into four treatments with six replicate pens per treatment and three piglets per pen (n = 18). These four treatment diets were formulated by adding 0, 250, 500 and 750 mg/kg WEAO to the basal diet. The experiment lasted for 28 days. Body weight and feed intake were measured. Blood samples were collected to determine immune and antioxidative parameters. The experimental results showed that WEAO supplementation improved the apparent nutrient digestibility of piglets in a linear or quadratic dose‐dependent manner. In addition, dietary WEAO quadratically increased serum concentrations of cytokines interleukin (IL)‐1, IL‐4, tumour necrosis factor (TNF)‐α, soluble surface antigen CD8 (sCD8), immunoglobulins (Ig)‐A and linearly increased serum concentrations of IL‐2, IL‐6, IgG, IgM. Furthermore, dietary WEAO linearly or quadratically decreased serum concentrations of malondialdehyde but quadratically increased activities of antioxidant enzymes and total antioxidative capacity. These results suggested that WEAO may prove useful as a natural phytogenic feed additive with antioxidative potential and could be incorporated into diets of piglets.
Simple SummaryConsidering that the photoperiod can affect melatonin (MLT) secretion and MLT can be used as reactive oxygen species scavenger and immunomodulator in animals, the present experiment was designed and conducted to study the effects of photoperiod change on MLT secretion, immune function and antioxidant status of cashmere goats. The results showed that the photoperiod of 8 h light and 16 h dark per day resulted in goats having a higher concentration of MLT and could effectively enhance the immune function and antioxidant enzyme activity of goats.AbstractThe photoperiod affects animals’ secretion of hormones, especially melatonin (MLT), which is involved in the regulation of the immune function and antioxidant status. The present experiment was conducted to study the effects of the photoperiod on MLT secretion, immune function, antioxidant status and related gene expression in goats. Eighteen adult female cashmere goats were randomly divided into three photoperiod groups: the control group (CG: natural photoperiod); the short-day photoperiod group (SDPP group: 8 h light; 16 h dark) and the shortening-day photoperiod group (SIPP group: lighting time shortened gradually from 16 h/d to 8 h/d). The experiment lasted for 60 days. The results showed that SDPP increased MLT concentration in serum at day 30 of the experiment (p < 0.05), but SIPP increased it at day 60 (p < 0.05). The activity of total superoxide dismutase (T-SOD), glutathione peroxidase (GPx) and catalase (CAT) increased (p < 0.05), and malondialdehyde (MDA) concentration decreased (p < 0.05) at day 30 in SDPP; no significant effects of SIPP were observed at day 30. Both SDPP and SIPP goats had higher activities of T-SOD, GPx and CAT (p < 0.05) at day 60. The concentration of immunoglobulin G (IgG), interleukin 1β (IL-1β) and interleukin 2 (IL-2) increased in SDPP (p < 0.05) at day 30. Both SDPP and SIPP raised the concentration of IgG, IL-1β and IL-2 at day 60 (p < 0.05). For the relative gene expression, the SDPP improved the gene expression of SOD1, CAT, GPx4, nuclear factor erythroid-2-related factor 2(Nrf2), IL-1β, IL-2 and tumor necrosis factor-α (TNF-α) (p < 0.05) in blood leukocytes at day 30. In addition, at day 60, goats in the SDPP group had a higher gene expression of CAT, GPx4, IL-1β and IL-2 (p < 0.05). Goats in SIPP had significantly higher gene expression of SOD1, CAT, GPx4, Nrf2, TNFα, IL-1β and IL-2 (p < 0.05) than those in CG. These results indicated that SDPP and SIPP could secrete more MLT and then improve the immune function and antioxidant status of the goats.
Cold climates may be a risk to the health and welfare of lambs during winter because cold environments alter the physiological processes of lambs, and we used cold environments with three different temperature gradients—an indoor heating control group (IHC) using electric heating; an indoor temperature group (IT) with intermittent and slight degrees of stimulation of coldness; an outdoor temperature group (OT) exposed to cold environments in an external natural environment. The results showed that the lambs in the OT group had a greater decrease in the average daily gain (ADG) and increase in the average daily feed intake (ADFI) and the feed-to-gain ratio (F:G) compared to the other two groups. The decrease in immunoglobulin A (IgA) and interleukin 2 (IL-2) contents and IL-2 gene expression, and the increase in tumor necrosis factor α (TNF-α) content and TNF-α and nuclear factor kappa-B p65 (NF-κB p65) gene expressions in the OT group suggested that the lambs had a compromised immune status in cold environments. Moreover, the decrease in catalase (CAT), glutathione peroxidase (GPx), total superoxide dismutase (T-SOD), and total antioxidant capacity (T-AOC) levels, and CAT, GPx, SOD1, SOD2, and nuclear factor-erythroid 2-related factor 2 (Nrf2) gene expressions, and the increase in malondialdehyde (MDA) in the OT group suggested that the lambs had a lower antioxidant defense capacity in cold environments. Thus, in extreme cold, lambs kept outdoors could reduce growth, immune function and antioxidant status. However, shelter feeding in winter could relieve the stress of cold environments on lambs, and housing with heating equipment was more conducive to the improve growth, immune, and antioxidant function of the lambs.
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