Methanol extract from mycelium of endophytic fungus Rhizoctonia sp. induces antinociceptive and anti-inflammatory activities in mice

Barros, Betijane Soares de; Silva, Juliane Pereira da; Ferro, Jamylle Nunes de Souza; Agra, Isabela Karine Rodrigues; Brito, Fabíola de Almeida; Albuquerque, Érica Dutra; Caetano, Luiz Carlos; Barreto, Emiliano

doi:10.1007/s11418-011-0534-x

Cited by 20 publications

(12 citation statements)

References 26 publications

(28 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Mice were injected with formalin (2.5%, 20 μL) in the subplantar area of the right hind paw. The time of paw licking (in seconds) was determined over 0–5 min (early phase, neurogenic) and 15–30 min (late phase, inflammatory) after formalin injection . Animals ( n = 6) were pretreated with CGP (0.1, 1, and 10 μmol/kg, i.p.)…”

Section: Methodsmentioning

confidence: 99%

“…Inflammation was induced by subcutaneous injection of 0.1 mL of 1% freshly prepared suspension of carrageenan into the hind paws of the mice. Following administration of carrageenan, the volume of the injected and counter‐lateral paws was measured at 30, 60, 120, and 240 min using a plethysmometer . The test groups were administered cyclic‐Gly‐Pro (CGP, 10 μmol/kg, i.p.)…”

Section: Methodsmentioning

confidence: 99%

“…The time of paw licking (in seconds) was determined over 0-5 min (early phase, neurogenic) and 15-30 min (late phase, inflammatory) after formalin injection. 51 Animals (n = 6) were pretreated with CGP (0.1, 1, and 10 lmol/kg, i.p.) 60 min before intraplantar injection of the stim- ulus or the reference compound, morphine (17.5 lmol/kg, i.p.…”

Section: Formalin-induced Nociception In Micementioning

confidence: 99%

“…The writhing test was carried out according to the method previously described. 51 Abdominal writhing in mice was caused by intraperitoneal (i.p.) injection of 0.1 mL of 0.6% acetic acid/10 g body weight.…”

Section: Abdominal Writhing Testmentioning

confidence: 99%

See 3 more Smart Citations

Cyclo‐Gly‐Pro, a cyclic dipeptide, attenuates nociceptive behaviour and inflammatory response in mice

Ferro

Aquino

Brito

et al. 2015

Clin Exp Pharma Physio

Self Cite

View full text Add to dashboard Cite

The present study aimed to investigate the antinociceptive and anti-inflammatory effects of the cyclic dipeptide cyclo-Gly-Pro (CGP) in mice. Antinociceptive activity was assessed by employing different pain models, such as formalin test, acetic acid-induced writhing, hot plate test, and carrageenan-induced hyperalgesia, in mice. The number of c-Fos-immunoreactive cells in the periaqueductal gray (PAG) was evaluated in CGP-treated mice. Anti-inflammatory activity was evaluated using paw oedema induced by carrageenan, compound 48/80, serotonin, and prostaglandin E2 (PGE2) and analyzed by plethysmometry. Quantitation of myeloperoxidase (MPO) in the paw was carried out to analyze the presence of neutrophils in the tissue. Intraperitoneal injection of CGP produced a significant inhibition in both neurogenic and inflammatory phases of formalin-induced pain. The antinociceptive effect of CGP, evaluated in the acetic acid-induced writhing test, was detected for up to 6 h after treatment. Further, in the hot plate test, antinociceptive behaviour was evoked by CGP, and this response was inhibited by naloxone. Animals treated with CGP did not present changes in motor performance. In CGP-treated mice there was an increase in the number of c-Fos-positive neurons in the periaqueductal gray. In another set of experiments, CGP attenuated the hyperalgesic response induced by carrageenan. Furthermore, CGP also reduced the carrageenan-increased MPO activity in paws. In addition, CGP also reduced the paw oedema evoked by compound 48/80, serotonin, and PGE2 . Taken together, these results may support a possible therapeutic application of the cyclic dipeptide cyclo-Gly-Pro toward alleviating nociception and damage caused by inflammation conditions.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Formalin-induced Nociception In Micementioning

confidence: 99%

Section: Abdominal Writhing Testmentioning

confidence: 99%

See 2 more Smart Citations

Cyclo‐Gly‐Pro, a cyclic dipeptide, attenuates nociceptive behaviour and inflammatory response in mice

Ferro

Aquino

Brito

et al. 2015

Clin Exp Pharma Physio

Self Cite

View full text Add to dashboard Cite

show abstract

“…Estudos mostram que fungos endofíticos são capazes de produzir compostos que apresentam atividade antioxidante e anti-inflamatória (Huang et al, 2007;Tianpanich et al, 2010;de Barros et al, 2011). A avaliação de extratos produzidos por fungos endofíticos isolados de 29 espécies de plantas medicinais da China mostrou que estes apresentam atividade antioxidante pronunciada, que foi atribuída à presença de polifenóis e de compostos como o ácido clorogênico, quercetina-3-rutonosídeo, quercetina-3-ramnosídeo e antraquinona (Huang et al, 2007 (Klass, 1977;Tiwari et al, 2014).…”

Section: Produtos Naturais Antienvelhecimentounclassified

Avaliação da expressão gênica de colágeno em fibroblastos tratados com extratos de fungos endofíticos

Araújo¹

View full text Add to dashboard Cite

Skin aging process is very complex and can be classified as intrinsic aging, primarily determined by genetic factors, and extrinsic aging, induced by nutritional and behavioral factors, such as chronic exposure to ultraviolet radiation, excessive alcohol consumption, smoking and nutritional disorders. Skin aging is characterized by morphological and functional changes, which can cause wrinkling, scaling, dryness, pigmentation abnormalities and skin cancer. Some of the disorders that affect the skin aging are mainly due to oxidative stress promoted by reactive oxygen species that are able to change the balance between synthesis and degradation of the dermal extracellular matrix components, favoring degradation rather than the synthesis process. Natural products protect skin from damage induced by oxidative stress and may act in important pathways in the skin aging. Among the possible sources for obtaining natural products it is possible to highlight the endophytic fungi, which are able to produce a great diversity of natural compounds. The objective of this study was to evaluate the influence of treatment with metabolites produced by two endophytic fungi isolated from Bauhinia variegate leaves on genic expression of collagen type I, metalloproteinase type 1 and tissue inhibitor of metalloproteinase type 1 in human dermal fibroblasts exposed to oxidative stress. The results show that both fungi were able to produce polyphenolic compounds and, more specifically, flavonoids. Treatment of fibroblasts with the extract produced by one of the endophytic fungi increased the expression of collagen type I gene, reduced the expression of metalloproteinase type 1 gene and inhibited metalloproteinase type 2 activity, suggesting that metabolites produced by this fungus can be benefic in prevent skin aging.

show abstract

Antinociceptive and anti-inflammatory activity of the siaresinolic acid, a triterpene isolated from the leaves of Sabicea grisea Cham. & Schltdl. var. grisea

Oliveira

Araújo

Lemos³

et al. 2015

J Nat Med

View full text Add to dashboard Cite

In the present study, siaresinolic acid (siaresinol, SA) was isolated from the leaves of Sabicea grisea and studied to evaluate its antinociceptive and anti-inflammatory activity. The antinociceptive effect of SA was investigated in mice using different animal models to study pain. In the acetic acid-induced writhing test, intraperitoneal (i.p.) injection of SA (0.1, 1, and 10 mg/kg, i.p.) 1 h before a pain stimulus significantly reduced the nociceptive response (by 42.3, 68.2, and 70.9 %, respectively). Pretreatment with glibenclamide, but not with yohimbine, metoclopramide, ketanserin, or naloxone, restored the antinociceptive effect induced by SA in the writhing test, suggesting that the K(+)ATP channel pathway might be involved in its mechanism of action. In the formalin test, SA (1 mg/kg, i.p.) decreased licking time in the second phase only, thereby indicating an anti-inflammatory effect. In the hot plate test, there was no significant difference in nociceptive behavior. In the rota-rod test, it was verified that a high dose of SA (10 mg/kg, i.p.) did not affect the locomotor activity of mice. In the pleurisy model, induced by carrageenan, treatment with SA inhibited important events involved in inflammatory responses, namely leukocyte influx, plasma leakage, and increased inflammatory mediators (TNF-α, IL-1β, and chemokine CXCL1), in the pleural exudate. Additionally, SA itself was not cytotoxic when evaluated using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay in macrophages cultured for 24 h at concentrations ranging from 1 to 200 μg/mL. These results suggest, for the first time, that SA attenuates nociceptive behavior through mechanisms involving receptors for ATP-dependent potassium channels, in addition to suppressing acute inflammatory responses.

show abstract

Methanol extract from mycelium of endophytic fungus Rhizoctonia sp. induces antinociceptive and anti-inflammatory activities in mice

Cited by 20 publications

References 26 publications

Cyclo‐Gly‐Pro, a cyclic dipeptide, attenuates nociceptive behaviour and inflammatory response in mice

Cyclo‐Gly‐Pro, a cyclic dipeptide, attenuates nociceptive behaviour and inflammatory response in mice

Avaliação da expressão gênica de colágeno em fibroblastos tratados com extratos de fungos endofíticos

Antinociceptive and anti-inflammatory activity of the siaresinolic acid, a triterpene isolated from the leaves of Sabicea grisea Cham. & Schltdl. var. grisea

Contact Info

Product

Resources

About