Methanesulphonate utilization by a novel methylotrophic bacterium involves an unusual monooxygenase

Kelly, Donovan P.; Baker, Susan C.; Trickett, Jim; Davey, Margaret; Murrell, J. Colin

doi:10.1099/00221287-140-6-1419

Cited by 58 publications

(67 citation statements)

References 36 publications

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“…2.1 Reagents EB (>99%) was purchased from Merck and mineral medium (MinE-containing CaCl2·H2O, MgSO4, and (NH4)2SO4) was prepared according to Kelly et al (1994).…”

Section: Methodsmentioning

confidence: 99%

Sequential Application of Soil Vapor Extraction and Bioremediation Processes for the Remediation of Ethylbenzene-Contaminated Soils

Soares

Pinho

Albergaria

et al. 2011

Water Air Soil Pollut

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Soil vapor extraction (SVE) is an efficient, wellknown and widely applied soil remediation technology. However, under certain conditions it cannot achieve the defined cleanup goals, requiring further treatment, for example, through bioremediation (BR). The sequential application of these technologies is presented as a valid option but is not yet entirely studied. This work presents the study of the remediation using sequential SVE and BR. The obtained results allow the conclusion that: (1) SVE was sufficient to reach the cleanup goals in 63% of the experiments (all the soils with NOMC below 4%), (2) higher NOMCs led to longer SVE remediation times, (3) BR showed to be a possible and cost-effective option when EB concentrations were lower than 335 mg kgso −1 , and (4) concentrations of EB above 438 mg kgso −1 showed to of ethylbenzene (EB)-contaminated soils, with different soil water and natural organic matter (NOMC) contents, be inhibitory for microbial activity.

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“…2.1 Reagents EB (>99%) was purchased from Merck and mineral medium (MinE-containing CaCl2·H2O, MgSO4, and (NH4)2SO4) was prepared according to Kelly et al (1994).…”

Section: Methodsmentioning

confidence: 99%

Sequential Application of Soil Vapor Extraction and Bioremediation Processes for the Remediation of Ethylbenzene-Contaminated Soils

Soares

Pinho

Albergaria

et al. 2011

Water Air Soil Pollut

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show abstract

“…Mineral medium (containing CaCl2 ·H2O, MgSO4, and (NH4)2SO4) was prepared according to Kelly et al (1994).…”

Section: Reagentsmentioning

confidence: 99%

Biocomplementation of SVE to achieve clean-up goals in soils contaminated with toluene and xylene

Soares

Pinho

Albergaria

et al. 2013

Environ Monit Assess

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Soil vapor extraction (SVE) and bioremedia-tion (BR) are two of the most common soil remediation technologies. Their application is widespread; however, both present limitations, namely related to the efficien-cies of SVE on organic soils and to the remediation times of some BR processes. This work aimed to study the combination of these two technologies in order to verify the achievement of the legal clean-up goals in soil remediation projects involving seven different simulated soils separately contaminated with toluene and xylene. The remediations consisted of the application of SVE followed by biostimulation. The results show that the combination of these two technologies is effective and manages to achieve the clean-up goals imposed by the Spanish Legislation. Under the experimental conditions used in this work, SVE is sufficient for the remediation of soils, contaminated separately with toluene and xylene, with organic matter contents (OMC) below 4 %. In soils with higher OMC, the use of BR, as a complementary technology, and when the concentration of contaminant in the gas phase of the soil reaches values near 1 mg/L, allows the achievement of the clean-up goals. The OMC was a key parameter because it hindered SVE due to adsorption phenomena but en-hanced the BR process because it acted as a microor-ganism and nutrient source.

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“…Methylotroph strain M2 (4) was cultivated and maintained on mineral salts medium Min E (16) containing 40 mM MSA. To provide the large amounts of biomass required for protein purification studies an MSA-limited chemostat was set up to maintain a steady-state biomass of strain M2 (16). Cells were harvested by centrifugation (17,000 ϫ g) at 4ЊC, washed three times with 40 mM Tris-HCl buffer, pH 7.0, and finally resuspended in the same buffer.…”

Section: Methodsmentioning

confidence: 99%

“…The use of MSA as a sulfur source by microorganisms has been noted (41,42); however, the subsequent fate of the carbon released has not been investigated. Bacteria that are capable of using MSA as a methylotrophic carbon source for growth have been isolated from terrestrial, freshwater, and marine environments (4,16,40). One organism, strain M2, contained an inducible oxygenolytic enzyme requiring NADH for activity.…”

mentioning

confidence: 99%

Purification and molecular characterization of the electron transfer protein of methanesulfonic acid monooxygenase

1997

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Methanesulphonate utilization by a novel methylotrophic bacterium involves an unusual monooxygenase

Cited by 58 publications

References 36 publications

Sequential Application of Soil Vapor Extraction and Bioremediation Processes for the Remediation of Ethylbenzene-Contaminated Soils

Sequential Application of Soil Vapor Extraction and Bioremediation Processes for the Remediation of Ethylbenzene-Contaminated Soils

Biocomplementation of SVE to achieve clean-up goals in soils contaminated with toluene and xylene

Purification and molecular characterization of the electron transfer protein of methanesulfonic acid monooxygenase

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