Methane production from glycolate excreting algae as a new concept in the production of biofuels

Günther, Annika; Jakob, Torsten; Goss, Reimund; König, S.; Spindler, Daniel; Räbiger, Norbert; John, Saskia; Heithoff, Susanne; Fresewinkel, Mark; Posten, Clemens; Wilhelm, Christian

doi:10.1016/j.biortech.2012.06.120

Cited by 34 publications

(30 citation statements)

References 14 publications

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“…This stagnation disappeared from day 2 on under glycolate‐producing conditions (Figure b). It should be emphasized that this initial glycolate production rate was already three times higher than previously reported (Günther et al ., ). The glycolate concentration reached a value of 1.25 m m on the first day and up to 2.75 m m on the second day (Figure ).…”

Section: Resultsmentioning

confidence: 97%

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Glycolate from microalgae: an efficient carbon source for biotechnological applications

Taubert

Jakob

Wilhelm

2019

Plant Biotechnology Journal

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Summary Glycolate is produced in autotrophic cells under high temperatures and C i ‐limitation via oxygenation of ribulose‐1,5‐bisphosphate. In unicellular algae, glycolate is lost via excretion or metabolized via the C 2 cycle by consuming reductants, ATP and CO 2 emission (photorespiration). Therefore, photorespiration is an inhibitory process for biomass production. However, cells can be manipulated in a way that they become glycolate‐producing ‘cell factories’, when the ratio carboxylation/oxygenation is 2. If under these conditions the C 2 cycle is blocked, glycolate excretion becomes the only pathway of photosynthetic carbon flow. The study aims to proof the biotechnological applicability of algal‐based glycolate excretion as a new biotechnological platform. It is shown that cells of Chlamydomonas can be cultivated under specific conditions to establish a constant and long‐term stable glycolate excretion during the light phase. The cultures achieved a high efficiency of 82% of assimilated carbon transferred into glycolate biosynthesis without losses of function in cell vitality. Moreover, the glycolate accumulation in the medium is high enough to be directly used for microbial fermentation but does not show toxic effects to the glycolate‐producing cells.

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Section: Resultsmentioning

confidence: 97%

“…Based on these limitations, Günther et al . () have proposed a new approach to use glycolate excretion as a natural process of C‐milking from the cells. It has been shown that glycolate can be directly used for anaerobic fermentation to produce methane.…”

Section: Introductionmentioning

confidence: 99%

Glycolate from microalgae: an efficient carbon source for biotechnological applications

Taubert

Jakob

Wilhelm

2019

Plant Biotechnology Journal

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“…This in turn facilitates the proliferation of those taxa with constitutive or facultative carbon concentration mechanisms (CCM), such as cyanobacteria (Raven et al, 2011). Increased ratios of oxygenation to carboxylation induced by temperature will not only reduce C-assimilation but will also lead to carbon losses via glycolate excretion (Guenther et al, 2012;Vílchez et al, 1991).…”

Section: Dark Reactions: Carboxylation and Photorespirationmentioning

confidence: 99%

Title: Freshwater phytoplankton responses to global warming

Wagner

Fanesi

Wilhelm

2016

Journal of Plant Physiology

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“…The Chl a fluorescence yield reflects all changes in environmental conditions that impact the photosynthetic apparatus, such as light availability, nutrient status, temperature, and generally all changes in cellular metabolism forced on cells by stress. In this example, cells of C. reinhardtii shift from producing biomass to producing and excreting glycolate, which is intended for use in a subsequent anaerobic methanogenesis stage as a new concept of algae-based biogas production [137,138]. Measurements were carried out in a Mini-Plate reactor with working volume of 200 mL [139] equipped with temperature control, irradiated from one side with PAR intensity of 200 µE m −2 s −1 , and with total aeration flow of 150 mL min −1 .…”

Section: Case Study: Decrease In Quantum Yield Monitored By Online Pamentioning

confidence: 99%

Monitoring of Microalgal Processes

Havlík

Scheper

Reardon

2015

Microalgae Biotechnology

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Process monitoring, which can be defined as the measurement of process variables with the smallest possible delay, is combined with process models to form the basis for successful process control. Minimizing the measurement delay leads inevitably to employing online, in situ sensors where possible, preferably using noninvasive measurement methods with stable, low-cost sensors. Microalgal processes have similarities to traditional bioprocesses but also have unique monitoring requirements. In general, variables to be monitored in microalgal processes can be categorized as physical, chemical, and biological, and they are measured in gaseous, liquid, and solid (biological) phases. Physical and chemical process variables can be usually monitored online using standard industrial sensors. The monitoring of biological process variables, however, relies mostly on sensors developed and validated using laboratory-scale systems or uses offline methods because of difficulties in developing suitable online sensors. Here, we review current technologies for online, in situ monitoring of all types of process parameters of microalgal cultivations, with a focus on monitoring of biological parameters. We discuss newly introduced methods for measuring biological parameters that could be possibly adapted for routine online use, should be preferably noninvasive, and are based on approaches that have been proven in other bioprocesses. New sensor types for measuring physicochemical parameters using optical methods or ion-specific field effect transistor (ISFET) sensors are also discussed. Reviewed methods with online implementation or online potential include measurement of irradiance, biomass concentration by optical density and image analysis, cell count, chlorophyll fluorescence, growth rate, lipid concentration by infrared spectrophotometry, dielectric scattering, and nuclear magnetic resonance. Future perspectives are discussed, especially in the field of image analysis using in situ microscopy, infrared spectrophotometry, and software sensor systems.

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Methane production from glycolate excreting algae as a new concept in the production of biofuels

Cited by 34 publications

References 14 publications

Glycolate from microalgae: an efficient carbon source for biotechnological applications

Glycolate from microalgae: an efficient carbon source for biotechnological applications

Title: Freshwater phytoplankton responses to global warming

Monitoring of Microalgal Processes

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