Methane-Producing Microbial Community in a Coal Bed of the Illinois Basin

Stra̧poć, Dariusz; Picardal, Flynn W.; Turich, Courtney; Schaperdoth, Irene; Macalady, Jennifer L.; Lipp, Julius S.; Lin, Yu-Ju; Ertefai, Tobias F; Schubotz, Florence; Hinrichs, Kai‐Uwe; Mastalerz, María; Schimmelmann, Arndt

doi:10.1128/aem.02341-07

Cited by 244 publications

(120 citation statements)

References 63 publications

(55 reference statements)

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“…Since Shimizu et al (2007) initially reported the microbial methanogenesis and community structure in coal seam groundwater in Japan, microbial communities related to CBM have been studied worldwide (Midgley et al, 2010;Penner et al, 2010;Strapoć et al, 2008). However, most such studies used conventional molecular methods and sampled produced water drilled from CBM wells.…”

Section: Discussionmentioning

confidence: 99%

“…Several phylogenetic studies have been made regarding the microbial communities associated with CBM in such reservoirs as the Gippsland Basin (Midgley et al, 2010), the Illinois Basin (Strapoć et al, 2008), the northern Japan (Shimizu et al, 2007), and the western Canada (Penner et al, 2010). The most common archaeal genera detected in CBM reservoirs are Methanosarcina, Methanolobus, Methanobacteria, Methanocorpusculum, Methanosaeta, Methanococci, Methanoculleus, and Methanoregula.…”

Section: Introductionmentioning

confidence: 99%

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Pyrosequencing reveals the dominance of methylotrophic methanogenesis in a coal bed methane reservoir associated with Eastern Ordos Basin in China

Guo

Liu

et al. 2012

International Journal of Coal Geology

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a b s t r a c t a r t i c l e i n f oIt is generally believed that biogenic coal bed methane (CBM) is an end product of coal biodegradation by methanogenic archaea and syntrophic bacteria. In this work, the archaeal and bacterial communities of CBM reservoir associated with Ordos Basin in China were investigated using 454 pyrosequencing. Sampling produced water, coal and rock in the reservoir, a total of 46,598 sequence reads were obtained. All archaea were methanogens with the genus Methanolobus predominating. The genus consisted of 81.18% of pyrosequencing reads in water sample and > 99% in coal and rock samples. Although the phylum Proteobacteria was the main component of all samples, bacterial communities in coal and rock samples were similar at the genus level, which were distinctly separated with water sample. The results strongly suggested that methylotrophic methanogenesis governed the biogenic CBM formation. The separation of microbial communities between water and coal, rock samples should be considered when investigating the process of coal biodegradation and the generation of new biogenic CBM.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Pyrosequencing reveals the dominance of methylotrophic methanogenesis in a coal bed methane reservoir associated with Eastern Ordos Basin in China

Guo

Liu

et al. 2012

International Journal of Coal Geology

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“…Sampling gas and water produced in the Illinois Basin, Strapoć et al (2007Strapoć et al ( , 2008 demonstrated the predominance of biogenic CBM formed via hydrogentrophic methanogenesis by a population of Methanocorpusculum using geochemical and microbiological methods. It was found that methanogens were also present in water produced from other reservoirs, e.g., the Powder River Basin (Green et al 2008), the Gippsland Basin (Midgley et al 2010), and the Alberta Basin (Penner et al 2010).…”

Section: Introductionmentioning

confidence: 99%

Methylotrophic methanogenesis governs the biogenic coal bed methane formation in Eastern Ordos Basin, China

Guo

Liu

et al. 2012

Appl Microbiol Biotechnol

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To identify the methanogenic pathways present in a deep coal bed methane (CBM) reservoir associated with Eastern Ordos Basin in China, a series of geochemical and microbiological studies was performed using gas and water samples produced from the Liulin CBM reservoir. The composition and stable isotopic ratios of CBM implied a mixed biogenic and thermogenic origin of the methane. Archaeal 16S rRNA gene analysis revealed the dominance of the methylotrophic methanogen Methanolobus in the water produced. The high potential of methane production by methylotrophic methanogens was found in the enrichments using the water samples amended with methanol and incubated at 25 and 35°C. Methylotrophic methanogens were the dominant archaea in both enrichments as shown by polymerase chain reaction (PCR)-denaturing gradient gel electrophoresis (DGGE). Bacterial 16S rRNA gene analysis revealed that fermentative, sulfate-reducing, and nitrate-reducing bacteria inhabiting the water produced were a factor in coal biodegradation to fuel methanogens. These results suggested that past and ongoing biodegradation of coal by methylotrophic methanogens and syntrophic bacteria, as well as thermogenic CBM production, contributed to the Liulin CBM reserves associated with the Eastern Ordos Basin.

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“…The chemical and isotopic data have been interpreted by plotting hydrocarbon gas composition C 1 /(C 2 þ C 3 ) versus d 13 C-CH 4 (Bernard et al, 1978) and dD-CH 4 versus d 13 C-CH 4 (Schoell, 1983) on widely used conventional diagrams. Numerous microbiological approaches have also been used to understand the methane production processes in subsurface environments such as petroleum-contaminated aquifers (Gray et al, 2009), geothermal aquifers (Kimura et al, 2005;Mochimaru et al, 2007b), and coalbed aquifers (Shimizu et al, 2007;Strąpoć et al, 2008). Igari and Sakata (1989) measured stable carbon isotopic ratios of methane in natural gases associated with the accretionary prism in Southwest Japan, and concluded that the methane was of thermogenic origin.…”

Section: Introductionmentioning

confidence: 99%

Microbial methane production in deep aquifer associated with the accretionary prism in Southwest Japan

et al. 2009

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To identify the methanogenic pathways present in a deep aquifer associated with an accretionary prism in Southwest Japan, a series of geochemical and microbiological studies of natural gas and groundwater derived from a deep aquifer were performed. Stable carbon isotopic analysis of methane in the natural gas and dissolved inorganic carbon (mainly bicarbonate) in groundwater suggested that the methane was derived from both thermogenic and biogenic processes. Archaeal 16S rRNA gene analysis revealed the dominance of H 2 -using methanogens in the groundwater. Furthermore, the high potential of methane production by H 2 -using methanogens was shown in enrichments using groundwater amended with H 2 and CO 2 . Bacterial 16S rRNA gene analysis showed that fermentative bacteria inhabited the deep aquifer. Anaerobic incubations using groundwater amended with organic substrates and bromoethanesulfonate (a methanogen inhibitor) suggested a high potential of H 2 and CO 2 generation by fermentative bacteria. To confirm whether or not methane is produced by a syntrophic consortium of H 2 -producing fermentative bacteria and H 2 -using methanogens, anaerobic incubations using the groundwater amended with organic substrates were performed. Consequently, H 2 accumulation and rapid methane production were observed in these enrichments incubated at 55 and 65 1C. Thus, our results suggested that past and ongoing syntrophic biodegradation of organic compounds by H 2 -producing fermentative bacteria and H 2 -using methanogens, as well as a thermogenic reaction, contributes to the significant methane reserves in the deep aquifer associated with the accretionary prism in Southwest Japan.

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Methane-Producing Microbial Community in a Coal Bed of the Illinois Basin

Cited by 244 publications

References 63 publications

Pyrosequencing reveals the dominance of methylotrophic methanogenesis in a coal bed methane reservoir associated with Eastern Ordos Basin in China

Pyrosequencing reveals the dominance of methylotrophic methanogenesis in a coal bed methane reservoir associated with Eastern Ordos Basin in China

Methylotrophic methanogenesis governs the biogenic coal bed methane formation in Eastern Ordos Basin, China

Microbial methane production in deep aquifer associated with the accretionary prism in Southwest Japan

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