Methamphetamine Induces Apoptosis in Seminiferous Tubules in Male Mice Testis

Yamamoto, Yoshiko; Yamamoto, Keiichi; Hayase, Tamaki; Abiru, Hitoshi; Shiota, Kohei; Mori, Chisato

doi:10.1006/taap.2001.9330

Cited by 37 publications

(40 citation statements)

References 16 publications

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“…The effect of methamphetamine in sperm morphology and sperm concentration seems to be dose-dependent as seen in animals treated with high dose acutely. These results are consistent with the previous report that percentage of apoptotic tubules was increased in a dose-dependent manner (Yamamoto et al, 2002). Decreased normal sperm morphology and low sperm concentrations after treated with methamphetamine may be involved in its effect on inducing apoptotic activity in seminiferous tubules.…”

Section: Discussionsupporting

confidence: 94%

“…Numbers of apoptotic cell per tubule were significantly increased in acute and sub-acute treated animals respectively. These were in agreement with the study by Yamamoto et al (2002) who reported that methamphetamine induces apoptosis in seminiferous tubules in male mice testis 24 h after a single treatment with 5, 10 and 15 mg kg )1 methamphetamine. The highest numbers of apoptotic cells per tubule found in acute treated animals in this study indicated that methamphetamine can induce apoptosis in a dose-dependent manner.…”

Section: Discussionsupporting

confidence: 94%

“…The highest numbers of apoptotic cells per tubule found in acute treated animals in this study indicated that methamphetamine can induce apoptosis in a dose-dependent manner. These were also similar to the study by Yamamoto et al (2002) which indicated that the percentage of seminiferous tubules demonstrating apoptotic cells increased dose-dependently after treatment with methamphetamine. Recently, repeated methamphetamine administration caused a decrease in cell proliferation, induce apoptosis and reduce the proliferation/apoptosis ratio in male rat germ cells.…”

Section: Discussionsupporting

confidence: 91%

“…Apoptotic cells were increased in both spermatogonia and primary spermatocytes after repeated administration of methamphetamine (Alavi et al, 2008). These findings may reflect that the genotoxic effect of methamphetamine seems to be involved especially in spermatogenesis (Yamamoto et al, 2002). In addition, it has been suggested that serum testosterone concentration seemed to be changed during the time course of methamphetamine treatment (Shin et al, 1999).…”

Section: Discussionmentioning

confidence: 93%

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Methamphetamine induces abnormal sperm morphology, low sperm concentration and apoptosis in the testis of male rats

Nudmamud-Thanoi

Thanoi

2011

Andrologia

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Methamphetamine has been reported to be an important drug in the field of reproductive toxicology. The aim of this study was to investigate the effects of methamphetamine administrations on sperm morphology, sperm concentration and apoptotic activity inside seminiferous tubule in male rats. Rats were administered a dose of 8 mg kg(-1) , intraperitoneally (IP), for acute group and a dose of 4 mg kg(-1) , IP, once daily for 14 days for sub-acute group. Percentage of normal sperm morphology was decreased in acute group when compared with control. Total numbers of sperm count were significantly decreased in acute and sub-acute groups. Apoptotic activities were most abundant in the seminiferous tubules of acute treated animals with a highly significant increase in the number of apoptotic cells per tubule. Those effects of methamphetamine seem to be dose-dependent. The results suggest that methamphetamine not only works as drug of abuse in central nervous system, but also in gametogenesis of males.

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Section: Discussionsupporting

confidence: 94%

Section: Discussionsupporting

confidence: 94%

Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 93%

See 2 more Smart Citations

Methamphetamine induces abnormal sperm morphology, low sperm concentration and apoptosis in the testis of male rats

Nudmamud-Thanoi

Thanoi

2011

Andrologia

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“…Apoptosis in testis was quantitated using a scoring system based on the percentage of the seminiferous tubules with TUNEL-positive cells (Blanchard et al 1996, Richburg 2000, Yamamoto et al 2002. The percentages of seminiferous tubules with TUNEL-positive cells in 5-day hypoxic rats (P!0.01), 15-day hypoxic rats (P!0.01), and 30-day hypoxic rats (P!0.05) were more than that in control rats (Figs 3 and 4).…”

Section: Tunelmentioning

confidence: 97%

Hypobaric hypoxia causes deleterious effects on spermatogenesis in rats

Liao¹,

Cai²,

Chen³

et al. 2010

Reproduction

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The study was conducted to explore the effects of hypobaric hypoxia on spermatogenesis in rats. Adult male Wistar rats were randomly divided into four groups: three hypoxia-exposed groups and one normoxic control group. Rats in the normoxic control group were raised at an altitude of 300 m, while rats in the 5-, 15-, and 30-day hypoxic groups were raised in a hypobaric chamber simulating a high altitude of 5000 m for 5, 15, and 30 days respectively. Flow cytometry was used to detect the DNA content of testicular spermatogenic cells in rats. The apoptosis of germ cells in testis was analyzed by using TUNEL assay. Spermatogenesis was also evaluated by morphology. Flow cytometry analysis revealed that 5-30 days of hypobaric hypoxia exposure significantly reduced the percentage of tetraploid cell population in rat testis. After rats were exposed to hypobaric hypoxia for 30 days, the ratio of haploid and diploid cell populations in testis reduced significantly. Seminiferous tubules with apoptotic germ cell increased after exposure to hypoxia. Most apoptotic germ cells were spermatogonia and spermatocytes. Hypoxia also caused decrease of cellularity of seminiferous epithelium, degeneration and sloughing of seminiferous epithelial cells occasionally. The data suggest that hypobaric hypoxia inhibits the spermatogenesis in rats. Decrease of tetraploid spermatogenic cells (primary spermatocytes) induced by hypoxia is an important approach to suppress spermatogenesis. The apoptosis of primary spermatocytes and spermatogonia may contribute to the loss of tetraploid cell populations.

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