Metformin and sitagliptin combination therapy ameliorates polycystic ovary syndrome with insulin resistance through upregulation of lncRNA H19

Wang, Qiong; Shang, Jing; Zhang, Yun; Zhou, Wei

doi:10.1080/15384101.2019.1652036

Cited by 23 publications

(19 citation statements)

References 31 publications

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“…Zhang et al 36 LncRNA-MALAT1 -lncRNA-MALAT1 is involved in the pathogenesis of PCOS through TGFβ signaling in granulosa cells -A nice biomolecule for possible future RNA therapy, repeated in literature Wang et al 107 LncRNA-H19 -Metformin and sitagliptin combination therapy is effective for PCOS with insulin resistance through upregulation of lncRNA-H19. To summarize, co-treatment induced H19 expression via suppressing the PI3K/AKT-DNMT1 pathway.…”

Section: Team Main Lncrnas Notes and Findingsmentioning

confidence: 99%

microRNAs and long non‐coding RNAs as biomarkers for polycystic ovary syndrome

Tamaddon

Azimzadeh

Tavangar

2022

J Cellular Molecular Medi

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Polycystic ovary syndrome (PCOS) is known as the most common endocrine/metabolic disorder in women of reproductive age, where it is reported that PCOS affects 5 to 26 percent of women (based on the applied diagnostic criteria) around the world. 1,2 PCOS was found to be associated with different diseases such as metabolic syndrome, type 2 diabetes, hypertension, cardiovascular diseases and even ovulatory infertility. 3,4 There are four distinguished subtypes; inflammatory, hidden cause and finally, pill-induced PCOS and insulin-resistant (the most prevalent type). 5,6 Although the complete etiology of the syndrome remains unclear, the genetic, epigenetic, environmental factors and lifestyle have been associated with PCOS causality. In clinical and/or biochemical settings, diagnosis is based on whether the patient shows no less than two out of three main symptoms, including cysts in the ovaries, high androgen levels and irregular periods. Despite the controversial opinions of current diagnostic criteria of PCOS, an expert opinion from the ultrasound, pelvic exam and blood tests can confirm the diagnosis. 7,8 Blood tests for PCOS are mainly based on hormone levels and endocrine function,

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Section: Team Main Lncrnas Notes and Findingsmentioning

confidence: 99%

microRNAs and long non‐coding RNAs as biomarkers for polycystic ovary syndrome

Tamaddon

Azimzadeh

Tavangar

2022

J Cellular Molecular Medi

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“…Finally, the glass slide was rinsed slowly with tap water. Subsequently, the cell morphological changes of vaginal smears were observed with a microscope so as to determine the different stages of the estrous cycle [6] .…”

Section: Specimen Collectionmentioning

confidence: 99%

“…Then, the cells were incubated with goat anti-rabbit IgG for 30 minutes at 37 °C and stained with DAB for 5 minutes at room temperature. The OGCs were washed with PBS for three times for 5 minutes, and nally, they were observed under light microscopy [6] .…”

Section: Isolation and Identi Cation Of Ogcsmentioning

confidence: 99%

Effects of Nesfatin-1 and Wnt /β-Catenin Pathway on OGCs in PCOS

Ding¹,

D²,

K³

et al. 2020

Preprint

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Background Polycystic ovary syndrome is a complex disease related to the endocrine and metabolism. Its specific cause and pathogenesis have not been clear. Nesfatin-1 could not only regulate energy balance and glucose metabolism, but also affect the reproductive system. The Wnt/β-catenin signaling pathway affects follicle development, ovulation, corpus luteum formation, and steroid hormone production. Results Here, we studied the roles of nesfatin-1 and Wnt/β-catenin signaling pathway in the pathogenesis of polycystic ovary syndrome. Firstly, the human primary ovarian granulosa cells in vitro was cultured. The results showed that the apoptosis rate of ovarian granulosa cells in polycystic ovary syndrome patients was significantly higher than that of granular cells in normal people. Moreover, nesfatin-1 and Wnt/β-catenin pathway inhibitor IWR-1could inhibit the expressions of ovarian granulosa cells apoptosis genes and promote their proliferation, as well as nesfatin-1 affected the expressions of foxo3a and its downstream factors. Then, an in vitro culture system for ovarian granulosa cells (OGCs) was established by employing a rat model. The results are the same with those mentioned above. Conclusion This strongly proves that the nesfatin-1 participates in regulating the apoptosis and proliferation of granulosa cells by the Wnt/β-catenin pathway. According to the role of nesfatin-1 and IWR in polycystic ovary syndrome, nesfatin-1 and Wnt/β-catenin pathway can provide a guideline for the diagnosis and treatment of Polycystic ovary syndrome (PCOS).

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“…It has been documented that the progression of PCOS is followed by changes in the expression levels of several lncRNAs, suggesting the importance of lncRNAs in the development of PCOS [10]. For example, lncRNA BANCR has been discovered to be involved in the progression of PCOS by promoting cell apoptosis [28]; upregulation of lncRNA H19 attenuated insulin resistance in PCOS [24]; upregulation of lncRNA SRLR in plasma could distinguish PCOS patients from healthy females [13]. LncRNA HOXA transcript antisense RNA myeloid-specific 1 (HOTAIRM1), located in the 5′ end of homeobox a gene cluster, is a natural antisense transcript of HOXA1 gene, expressed in the myeloid lineage and induced during neuronal differentiation [16,31].…”

Section: Introductionmentioning

confidence: 99%

LncRNA HOTAIRM1, miR-433-5p and PIK3CD function as a ceRNA network to exacerbate the development of PCOS

Guo

Sun

2021

J Ovarian Res

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Background Currently, several non-coding RNAs (ncRNAs) were distinguished in polycystic ovarian syndrome (PCOS). This present study aims to explore the potential function of lncRNA HOTAIRM1/miR-433-5p/PIK3CD in ovarian granulosa cells. Methods We analyzed the expression profiles of HOTAIRM1, miR-433-5p and PIK3CD in PCOS samples by enquiring GEO database. GSEA was applied to enrich the pathways related to PCOS. The target association between HOTAIRM1 and miR-433-5p or the binding association between miR-433-5p and PIK3CD were assessed by online prediction tools and a dual luciferase reporter assay. qPCR and western blotting assays were used to detect PIK3CD expression after HOTAIRM1 and miR-433-5p treatment. The proliferation and apoptosis of ovarian granulosa cells were estimated by cell counting kit-8 and flow cytometry assays, respectively. Results The expression profiles of HOTAIRM1 and PIK3CD were increased, whereas miR-433-5p was decreased in PCOS tissues. PIK3CD expression was positively regulated by HOTAIRM1 and negatively modulated by miR-433-5p. Overexpression of HOTAIRM1 reduced the proliferative ability and increased the apoptotic ability of granulosa cells, whereas upregulation of miR-433-5p or downregulation of PIK3CD reversed the effects of HOTAIRM1 on granulosa cells. Moreover, overexpression of miR-433-5 displayed a results with increasing proliferative ability and decreasing apoptotic ability, but upregulation of PIK3CD eliminated the function of miR-433-5p on granulosa cells. Conclusions Our findings illustrated that HOTAIRM1 could sponge miR-433-5p to promote PIK3CD expression, thereby regulating the growth and apoptosis of granulose cells in PCOS.

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Metformin and sitagliptin combination therapy ameliorates polycystic ovary syndrome with insulin resistance through upregulation of lncRNA H19

Cited by 23 publications

References 31 publications

microRNAs and long non‐coding RNAs as biomarkers for polycystic ovary syndrome

microRNAs and long non‐coding RNAs as biomarkers for polycystic ovary syndrome

Effects of Nesfatin-1 and Wnt /β-Catenin Pathway on OGCs in PCOS

LncRNA HOTAIRM1, miR-433-5p and PIK3CD function as a ceRNA network to exacerbate the development of PCOS

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