Metataxonomic analyses reveal differences in aquifer bacterial community as a function of creosote contamination and its potential for contaminant remediation

Abstract

Metataxonomic approach was used to describe the bacterial community from a creosote-contaminated aquifer and to access the potential for in situ bioremediation of the polycyclic aromatic hydrocarbons (PAHs) by biostimulation. In general, the wells with higher PAH contamination had lower richness and diversity than others, using the Shannon and Simpson indices. By the principal coordinate analysis (PCoA) it was possible to observe the clustering of the bacterial community of most wells in response of the presence of PAH contamination. The significance analysis using edgeR package of the R program showed variation in the abundance of some Operational Taxonomic Units (OTUs) of contaminated wells compared to uncontaminated ones. Taxons enriched in the contaminated wells were correlated positively (p < 0.05) with the hydrocarbons, according to redundancy analysis (RDA). All these enriched taxa have been characterized as PAH degrading agents, such as the genus Comamonas, Geobacter, Hydrocarboniphaga, Anaerolinea and Desulfomonile . Additionally, it was possible to predict, with the PICRUSt program, a greater proportion of pathways and genes related to the degradation of PAHs in the wells with higher contamination levels. We conclude that the contaminants promoted the enrichment of several groups of degrading bacteria in the area, which strengthens the feasibility of applying biostimulation as an aquifer remediation strategy.

Divergent Fate and Roles of Dissolved Organic Matter from Spatially Varied Grassland Soils in China During Long-Term Biogeochemical Processes

“…At the genus level, the microbial communities were quite different for both regions (Figure S23). The enrichment of Rhodococcus, Sphingomonadaceae, Flavobacteriales, Polaromonas, and Methylotenera genera was observed for the high-HS regions (Figure S24), associated with the biodegradation of lignin-like and polyaromatic compounds. − Thauera and Hydrocarboniphaga genera were the key discriminating members for the low-HS regions, also related to the decomposition of aromatic components. , …”

“…56−60 Thauera and Hydrocarboniphaga genera were the key discriminating members for the low-HS regions, also related to the decomposition of aromatic components. 61,62 Further, the low-HS environments were more enriched in bacterial genes with potential functions for biosynthesis, including the biosynthesis of amino acids (valine, leucine, and isoleucine), aminoacyl-tRNA, streptomycin, and polyketide sugar unit (P < 0.05) (Figure 5c). However, microbes in high-HS environments were more specialized in biodegradation and metabolism, including the biodegradation of amino acids, ketones, styrene, aromatic compounds, and fatty acids (P < 0.05) (Figure 5c).…”

Using Network Analysis and Predictive Functional Analysis to Explore the Fluorotelomer Biotransformation Potential of Soil Microbial Communities

“…In Module 3, the genus Anaerolinea (ASV0939) and a group in family A4b (ASV0104) within the phylum Chloroflexota, and the genus Anaeromyxobacter of phylum Myxococcota (ASV 0654 and ASV2000), as well as the genus Pseudolabrys in the phylum Pseudomonadota (ASV0257), acted as module hubs. Anaerolinea species have been characterized as carbohydrate and PAH degraders. , Other potential keystone taxa that were not within Modules 1–3 included unclassified genera in family BSV40 from the phylum Bacteroidota (ASV1644) and order SBR1031 from the phylum Chloroflexota (ASV1379). Members in order SBR1031 have been documented to play a crucial role in degradation of biodegradable plastics and PAHs .…”

“…Anaerolinea species have been characterized as carbohydrate and PAH degraders. 103,104 Other potential keystone taxa that were not within Modules 1−3 included unclassified genera in family BSV40 from the phylum Bacteroidota (ASV1644) and order SBR1031 from the phylum Chloroflexota (ASV1379). Members in order SBR1031 have been documented to play a crucial role in degradation of biodegradable plastics and PAHs.…”

Salinity as a major influence on groundwater microbial communities in agricultural landscapes

“…Quantitative polymerase chain reaction (qPCR) was undertaken on a subset of eDNA samples on a Roche Light Cycler 480 using non-tagged primers, to assess for PCR inhibition and confirm DNA amplification. PCR was performed to amplify the V4 region of the 16S region of the rRNA gene for prokaryotes by using the primers 501FB -GTGYCAGCMGCCG CGGTAA and 809RB -GGACTACNVGGGTWTCTAAT (Júlio et al 2019). A 96-well plate with DNA samples and controls (seven blanks, two PCR negative controls and two PCR positive controls) was established where samples were arranged with a blank well in each column, and random interspersed positive (synthetic bacterial DNA) and negative (DNA-free H 2 O) controls to ensure that there was no systematic bias.…”