Metasurfaces‐Driven Hyperspectral Imaging via Multiplexed Plasmonic Resonance Energy Transfer

Abstract

Obtaining single–molecular–level fingerprints of biomolecules and electron–transfer dynamic imaging in living cells are critically demanded in postgenomic life sciences and medicine. However, the possible solution called plasmonic resonance energy transfer (PRET) spectroscopy remains challenging due to the fixed scattering spectrum of a plasmonic nanoparticle and limited multiplexing. Here, multiplexed metasurfaces‐driven PRET hyperspectral imaging, to probe biological light–matter interactions, is reported. Pixelated metasurfaces with engineered scattering spectra are first designed over the entire visible range by the precision nanoengineering of gap plasmon and grating effects of metasurface clusters. Pixelated metasurfaces are created and their full dark‐field coloration is optically characterized with visible color palettes and high‐resolution color printings of the art pieces. Furthermore, three different biomolecules (i.e., chlorophyll a, chlorophyll b, and cytochrome c) are applied on metasurfaces for color palettes to obtain selective molecular fingerprint imaging due to the unique biological light–matter interactions with application‐specific biomedical metasurfaces. This metasurface‐driven PRET hyperspectral imaging will open up a new path for multiplexed real‐time molecular sensing and imaging methods.

Metasurfaces for Quantitative Biosciences of Molecules, Cells, and Tissues: Sensing and Diagnostics

“…Metasurfaces employed for quantum object sensing, e.g., electron transfer monitoring between molecules and metasurfaces, require the engineerable plasmon resonance wavelength matching the absorption maximum of the electron acceptor molecule. The plasmon resonance tuning in the visible can be effectively performed via designing aluminum nanodisk array geometries . However, future metasurface-based studies could explore the potential of metasurface to analyze quantum biological effects with present spatial information, which could be applied for cancer therapeutics, cell life cycle monitoring, and dynamic monitoring of enzyme activity of live bacteria or cells …”

“…The plasmon resonance tuning in the visible can be effectively performed via designing aluminum nanodisk array geometries. 51 However, future metasurfacebased studies could explore the potential of metasurface to analyze quantum biological effects with present spatial information, which could be applied for cancer therapeutics, 50 cell life cycle monitoring, 48 and dynamic monitoring of enzyme activity of live bacteria or cells. 102 We also highlight current advances in monitoring cell morphology, secretion, and tissue section analysis as diagnostic methods.…”

“…This control over quantum biological functions enables the selective triggering of cancer cell apoptosis as a potential therapeutic treatment. In contrast, plasmonic metasurfaces enable multiplexed molecule sensing and electron transfer via gap plasmon resonance engineering (Figure b) . The metasurface can cover the entire cell area and enable the real-time monitoring of cell activity with high spatial resolution.…”

“…In contrast, plasmonic metasurfaces enable multiplexed molecule sensing and electron transfer via gap plasmon resonance engineering (Figure 3b). 51 The metasurface can cover the entire cell area and enable the real-time monitoring of cell activity with high spatial resolution.…”

A mid-IR tunable graphene metasurface for ultrasensitive molecular fingerprint retrieval and refractive index sensing

“…The continuous near-field enhancement over a broad spectrum is advantageous for addressing this issue. 21–23 Hatice Altug et al demonstrated imaging-based molecular barcoding with pixelated dielectric metasurfaces and achieved chemically specific broadband IR detection by integrating a range of high- Q resonances by simply adjusting the geometric parameters. 24 Furthermore, angle-multiplexed all-dielectric metasurfaces are designed, where the resonance frequency can be tuned by controlling the incidence angle of light.…”