Metastatic potential of transformed rat 3Y1 cell lines is inversely correlated with lysosomal‐type sialidase activity

Abstract

We have investigated sialidase activities in transformed rat 3Y 1 cells of different metastatic potential. Only lysosome-type sialidase was apparent in the particulate fractions of 3Yl cells and their transformants. As compared with control 3Y1 cells, src-transformed cells exhibited decreased sialidase activity, and v-for transfer to these latter induced even more severe decrease in the sialidase activity with acquisition of high lung metastatic ability. Various lysosomal enzymes other than sialidase were hardly affected by the transformation. Sialic acid transfer to N-linked glycoproteins was slightly elevated in the transformants, but not in parallel with their metastatic potential.

Overexpression of lysosomal-type sialidase leads to suppression of metastasis associated with reversion of malignant phenotype in murine B16 melanoma cells

“…Taken together, the present results strengthen our previous data 31 showing that lysosomal sialidase affects malignant properties including metastatic ability of the cancer cells; modulation of this gene expression would open up the possibility of potential applications in cancer therapy.…”

“…31 This tendency was also observed in B16 melanoma variants, even with a low level of endogenous activity. 10 To understand lysosomal sialidase function in cancer metastasis, we attempted to obtain a stable transfectant by introduction of this sialidase into highly metastatic cells.…”

Contribution of sialidase NEU1 to suppression of metastasis of human colon cancer cells through desialylation of integrin β4

“…We previously demonstrated that Neu1 would have an important function in glycoprotein and oligosaccharide catabolism (Miyagi and Tsuiki, 1984) and that the expression level correlates inversely with metastatic capacity in rat 3Y1 transformants (Miyagi et al, 1994) and in mouse adenocarcinoma colon 26 cells (Sawada et al, 2002) of different metastatic potentials. To investigate whether overexpression of Neu1 can reverse metastatic capacity, we next introduced the gene into B16 melanoma cells, and found that sialidase overexpression resulted in suppression of experimental pulmonary metastasis (Kato et al, 2001).…”

Suppression of pulmonary metastasis in murine B16 melanoma cells by transfection of a sialidase cDNA

“…Despite numerous reports on the involvement of sialic acids in metastasis, it remains uncertain how such aberrant sialylation occurs in malignant cells and why such changes affect metastasis. In a previous study (Miyagi et al, 1994), we found that metastatic potential was inversely correlated with an endogenous sialidase activity in transformed rat 3Y1 cell lines, whereas it did not have a significant relation with sialic acid levels. These results led us to the idea that the alteration of sialidase expression may be important for metastasis.…”