Metaphase arrest and delay in cell cycle kinetics of root apical meristems and mouse bone marrow cells treated with leaf aqueous extract of Clerodendrum viscosum Vent

Abstract

It may be said that LAECV contains mitostatic and metaphase arresting components that are able to induce significant metaphase arrest in root apical meristems and also in mouse bone marrow cells.

Antiproliferative Activity of Allelochemicals Present in Aqueous Extract of Synedrella nodiflora (L.) Gaertn. In Apical Meristems and Wistar Rat Bone Marrow Cells

“…Such a reduction in mitotic index suggests that exposure to AESN led to the cell cycle disturbances and decrease in cell number entering mitotic division. In the present and our previous study, onion root apical meristem cells were found to be excellent cell system for cell cycle kinetics analysis [26] . Levan first introduced A. cepa root tip assay and later it was used as a standard method to study genotoxicity [33][34][35][36][37][38] .…”

“…In green gram seedlings, the root growth retardation increased from 2 to 77% for the increased concentration from 0.5 to 6 mg/ml (p<0.001). Our previous study showed colchicine, a metaphase arresting agent, also induced growth retardation in green-gram seedlings in a dose dependent manner [26] . The .…”

“…These results are in agreement with the previous study reports indicating the root growth retardation is a result of the suppression of cell division [27] . A number of earlier studies have suggested that the level of root growth inhibition increases with the increasing extract concentrations [26], [28] .…”

“…All the AESN treatment doses (100, 300 and 500 mg/kg body weight) could induce cell cycle delay in interphase which may be the reason of reduced number of cells at mitotic phases, hence, less number of cells were available to be arrested at the metaphase with the colchicine actions. In an another study, similar antiproliferative effects of leaf aqueous extracts from a traditionally used medicinal plant (Clerodendrum viscosum), revealed antiproliferative, metaphase arresting and apoptosis inducing activities in both the plant and animal cell systems [26], [39] . Aqueous extract from the leaves of Toona sinensis has been shown to have an antiproliferative effect on human lung cancer cells [40], [41] .…”

Allelopathic Effects of Clerodendrum Inerme Gaertn. And Clerodendrum Viscosum Vent. On Two Crop Plants Cicer Arietinum L. And Lathyrus Sativus L.

“…The reduction in mitotic index might be explained as hindrance before the onset of prophase, the arrest of one or more mitotic phases or slowing down the cell cycle progression during mitosis. Studies on Allium root tip cells and mouse bone narrow cells exhibit decreased mitotic activity with Clerodendrum extracts 28 . The antimitotic activity of root tip cells was exhibited by a number of plant extracts 29,30 .…”