Cytogenotoxic effects of 3-epicaryoptin in Allium cepa L. root apical meristem cells

Barman, Manabendu; Ray, Swati

doi:10.1007/s00709-023-01838-6

Cited by 5 publications

(4 citation statements)

References 65 publications

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“…The study of chromosomal anomalies is another parameter used to determine the cytogenotoxic potential of the tested substances [ 77 ]. In our study, chromosomal anomalies were observed during metaphase, anaphase, and telophase.…”

Section: Discussionmentioning

confidence: 99%

Effects of Indigo Carmine on Growth, Cell Division, and Morphology of Allium cepa L. Root Tip

Ristea,

Zarnescu

2024

Toxics

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Indigo carmine has a variety of uses in foods, textiles, medicine, pharmaceuticals, and cosmetics. There are studies reporting the toxic potential of indigo carmine on human health and the environment. In this study, we investigated the cytogenotoxic effects of indigo carmine using apical root cells of Allium cepa. Allium cepa bulbs were subjected to four treatments with indigo carmine (0.0032, 0.0064, 0.0125, and 0.2 mg/mL) and to ultrapure water as a control. After 5 days, root growth, root length, mitotic index, mitotic inhibition, chromosomal anomalies, and cell morphology were analyzed. According to our results, a decrease in root length and mitotic index was observed at all concentrations of indigo carmine. Additionally, several types of chromosomal abnormalities were observed, such as disturbed metaphase, sticky chain metaphase, anaphase bridge, and laggard chromosomes. Moreover, histological observation indicated that indigo carmine induces alterations in various components of root tip tissue, such as deformation and alteration of the cell wall, progressive condensation of chromatin, shrinkage of the nuclei, and an increase in the number of irregularly shaped nuclei and nuclear fragments. Our results indicate that the tested concentrations of indigo carmine may have toxic effects and raise concerns about its intensive use in many fields.

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Section: Discussionmentioning

confidence: 99%

Effects of Indigo Carmine on Growth, Cell Division, and Morphology of Allium cepa L. Root Tip

Ristea,

Zarnescu

2024

Toxics

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“…Anti-tubulin antibody and Goat anti-mouse IgG-FITC used were obtained from Santa Cruz, the USA. 3-epicaryoptin was isolated from leaves of Clerodendrum inerme [26].…”

Section: Materials Methodsmentioning

confidence: 99%

“…In our previous comparative cytotoxicity analysis on a plant test system (Allium cepa root apical meristem cells) was shown that the compound 3-epicaryoptin isolated from the leaves of C. inerme has colchicine-like effects, including root tip swelling, metaphase arresting, micronuclei, and polyploidy inducing effects [25][26][27][28][29]. However, whether 3-epicaryoptin regulates the cell cycle in human breast carcinoma remains unclear.…”

Section: Introductionmentioning

confidence: 99%

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3-epicaryoptin induces G2/M phase cell cycle arrest and apoptosis in human breast cancer cells by disrupting the microtubule network, anin vitroandin silicoinvestigation

Barman,

Roy,

Singh

et al. 2024

Preprint

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Breast cancer (BC) is a prevalent form of cancer observed in women across the globe, constituting over a quarter of all female BC cases. The treatment of BC continues to require significant efficacy, aiming to achieve high success rates while minimizing adverse effects on the body as a whole. In the current study, 3-epicaryoptin was tested for the molecular mechanism of its anti-cancer activity in the human breast cancer cell line, MCF-7. We investigated cell viability by MTT assay, cell cycle kinetics and apoptosis, immunofluorescence straining, molecular modelling, and ADMET profiling. MTT assay results showed that 3-epicaryoptin was found cytotoxic against MCF-7 cells with an IC50 value of 0.345 mg mL-1 for 48 h. Flow cytometric analysis exhibited that 3-epicaryoptin halted the MCF-7 cells in the G2/M phase and subsequently induced apoptosis in a time-dependent manner. Our immunofluorescence studies indicated that 3-epicaryoptin inhibited microtubule polymerization in MCF-7 cells. Furthermore, molecular docking followed by molecular dynamics (MD) simulation studies demonstrated the ability of 3-epicaryoptin to interact with the tubulin protein at the colchicine binding pockets. Overall, our results suggest that 3-epicaryoptin can inhibit the proliferation of human breast cancer cells by depolymerizing of cellular microtubule networks, which causes cell cycle arrest and promotes apoptotic cell death. Therefore, it has been indicated that the natural product 3-epicaryoptin exhibited considerable promise as a potent therapeutic agent capable of inducing apoptosis in breast cancer cells.

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Clerodendrum inerme (L.) Gaertn.: a critical review on current progress in traditional uses, phytochemistry, pharmacological aspects and toxicity

Barman,

Ray

2024

Phytochem Rev

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Cytogenotoxic effects of 3-epicaryoptin in Allium cepa L. root apical meristem cells

Cited by 5 publications

References 65 publications

Effects of Indigo Carmine on Growth, Cell Division, and Morphology of Allium cepa L. Root Tip

Effects of Indigo Carmine on Growth, Cell Division, and Morphology of Allium cepa L. Root Tip

3-epicaryoptin induces G2/M phase cell cycle arrest and apoptosis in human breast cancer cells by disrupting the microtubule network, anin vitroandin silicoinvestigation

Clerodendrum inerme (L.) Gaertn.: a critical review on current progress in traditional uses, phytochemistry, pharmacological aspects and toxicity

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