Metallothionein Isoform Expression in Benign and Malignant Thyroid Lesions

Wojtczak, Beata; Puła, Bartosz; Gomułkiewicz, Agnieszka; Olbromski, Mateusz; Podhorska-Okołów, Marzena; Domosławski, Paweł; Bolanowski, Marek; Daroszewski, Jacek; Dzięgiel, Piotr

doi:10.21873/anticanres.11940

Cited by 8 publications

(6 citation statements)

References 23 publications

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“…Some studies have shown that increased expression of MT is associated with a higher proliferative potential in various types of cancer and a more rapid disease progression, as well as cellular resistance to chemotherapy and radiotherapy 15,16 . However, there is a paucity of studies evaluating the immunohistochemical expression of MT in breast cancer, and to the best of our knowledge, only one published study in the literature has compared the expression of MT-1 between women with breast cancer and women with fibroadenoma 14 .…”

Section: Introductionmentioning

confidence: 99%

A case-control study of Metallothionein-1 expression in breast cancer and breast fibroadenoma

Sampaio

Martins

Dourado

et al. 2019

Sci Rep

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The overexpression of Metallothionein-1 (MT-1) may play an important role in breast cancer; however, few studies have compared MT-1 expression between breast cancer and fibroadenoma. A cross-sectional controlled study was performed in 66 premenopausal women, aged 20–49 years, who had been histologically diagnosed with breast fibroadenoma or breast cancer. The patients were divided into two groups: group A, control (fibroadenoma, n = 36) and group B, study (breast cancer, n = 30). Immunohistochemistry was performed on tissue samples of fibroadenoma and breast cancer patients to evaluate the expression of metallothionein using an anti-MT-1 polyclonal antibody (rabbit polyclonal anti-metallothionein-Catalog Number biorbyt-orb11042) at a dilution of 1:100. The data were analyzed using NOVA (p < 0.05). Microscopic analysis showed a higher concentration of anti-MT-1-stained nuclei in breast cancer tissues than in fibroadenoma tissues. The mean proportion of cells with anti-MT-1-stained nuclei was 26.93% and 9.10%, respectively, in the study and control groups (p < 0.001). Histological grade 3 tumors showed a significantly higher MT-1 expression than hitological grade 1 (p < 0.05), while breast tumors negative for estrogen-, progesterone- and HER2- receptors had a significantly higher MT-1 expression than positive breast tumors positive for these parameters (p < 0.05). MT-1 protein in women of reproductive age was significantly higher in breast cancer than in fibroadenoma in this study. Furthermore, there was higher MT-1 immunoreactivity in more aggressive tumors.

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Section: Introductionmentioning

confidence: 99%

A case-control study of Metallothionein-1 expression in breast cancer and breast fibroadenoma

Sampaio

Martins

Dourado

et al. 2019

Sci Rep

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“…Beata Wojtczak et al. ( 32 ) has found that metallothioneins (MTs) are involved in many cellular processes, such as the binding and transport of zinc and copper ions, and the expression of functional MT genes may contribute to thyroid carcinogenesis. Jorge Gaspar et al.…”

Section: Discussionmentioning

confidence: 99%

Assessment of causal association between differentiated thyroid cancer and disordered serum lipid profile: a Mendelian randomization study

Ma,

Li,

et al. 2023

Front. Endocrinol.

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BackgroundResearch has shown that the disordered serum lipid profile may be associated with the risk of differentiated thyroid cancer (DTC). Whether this association reflect causal effect is still unclear. The aim of this study was to evaluate the causality of circulating lipoprotein lipids on DTC.MethodsMendelian randomization (MR) analysis was conducted to evaluate the relationship between the circulating lipoprotein lipids and DTC risk using single-nucleotide polymorphisms (SNPs) from a genome-wide association (GWA) study containing a high-incidence Italian population of 690 cases samples with DTC and 497 controls.ResultsUnivariate and multivariate mendelian randomization analysis demonstrated that ‘total cholesterol’, ‘HDL cholesterol’, ‘apolipoprotein B’ and ‘ratio of apolipoprotein B to apolipoprotein A1’ were correlated with DTC. According to sensitivity analysis, our results were reliable. Furthermore, multivariate analysis revealed that there is no causative association between DTC and any of the many cause factors when they interact with one another, suggesting that there was a deep interaction between the four factors, which could affect each other. Finally, the mechanism of the related effects each other as well as the target genes with significant SNP regulatory effects in DTC was explored by conducting functional enrichment analysis and constructing the regulatory networks.ConclusionsWe obtained four exposure factors (total cholesterol, HDL cholesterol, apolipoprotein B and ratio of apolipoprotein B to apolipoprotein A1) closely related to DTC, which laid a theoretical foundation for the treatment of DTC.

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“…17 Its protein product is a low-molecular weight, cysteine-rich protein that acts as a scavenger of free radicals and heavy metals. 17 Aberrant expression of the MT1F gene has been linked to various human cancers, including colon cancer, 18 hepatocellular carcinoma, 19 and gastric cancer, 20 suggesting its potential role in cancer development and progression. However, the above two genes have not been studied as specific markers of ECs.…”

Section: Discussionmentioning

confidence: 99%

“…The results demonstrate that CA4 may serve as a diagnostic biomarker and potential therapeutic target in GC by inhibiting cellular proliferation via regulating the expression of cell cycle‐associated proteins. The MT1F gene is a member of the metallothionein (MT) gene family 17 . Its protein product is a low‐molecular weight, cysteine‐rich protein that acts as a scavenger of free radicals and heavy metals 17 .…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic landscape of endothelial cells: Key tumor microenvironment components indicating variable clinical outcomes in pancreatic ductal adenocarcinoma

Xie

Tan

et al. 2023

Environmental Toxicology

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Endothelial cells (ECs) present in the tumor microenvironment (TME) exhibit significant diversity that may impact the efficacy of anti‐tumor treatments. Thus, our study sought to elucidate the various clusters of ECs present in pancreatic ductal adenocarcinoma (PDAC) and explore their possible interactions and influence on clinical outcomes. We obtained single‐cell transcriptome data from 24 PDAC tumors and 11 normal pancreases, minimizing any batch effects between samples. Next, we compared the relative abundance of various ECs clusters across distinct sample types. Pseudo‐time analysis was employed to investigate the differentiation origin of ECs. A variety of bioinformatics methods were used to investigate potential communication between ECs and malignant cells, as well as assess metabolic changes, pathway alterations, and immune‐related markers expression within distinct EC clusters. Lastly, we investigated the impact of particular ECs clusters on patient prognosis in bulk transcriptome data. Our study identified seven distinct clusters of ECs, denoted as CA4+ ECs, MMP2+ ECs, SPP1+ ECs, MT1F+ ECs, CCL5+ ECs, RGS5+ ECs, and TYROBP+ ECs. Pseudo‐time analysis suggested that the loss of CA4+ ECs and MT1F+ ECs may promote malignant progression. Cell communication elucidated that MT1F+ ECs exhibited the strongest outgoing interaction strength, whereas RGS5+ ECs displayed the strongest incoming interaction strength. Furthermore, TYROBP+ ECs exhibited greater metabolic activity, and notably, CCL5+ ECs displayed increased expression of immune‐related molecules. Lastly, across cohorts of bulk transcriptome levels, CA4+ ECs, MT1F+ ECs, and RGS5+ ECs consistently demonstrated prognostic indicative effects. PDAC patients exhibit the presence of seven distinct EC clusters, each demonstrating significant metabolic and immunological heterogeneity. Targeted therapeutic approaches directed toward CA4+ ECs and MT1F+ ECs may prove advantageous in addressing challenges associated with PDAC treatment. Additionally, variations in the relative abundance of CA4+ ECs, MT1F+ ECs, and RGS5+ ECs were indicated as predictive of patient prognosis.

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Metallothionein Isoform Expression in Benign and Malignant Thyroid Lesions

Abstract: Expression of functional MT isoforms may contribute to thyroid carcinogenesis and potentially serve as a diagnostic marker in distinguishing benign and malignant lesions.

Cited by 8 publications

References 23 publications

A case-control study of Metallothionein-1 expression in breast cancer and breast fibroadenoma

A case-control study of Metallothionein-1 expression in breast cancer and breast fibroadenoma

Assessment of causal association between differentiated thyroid cancer and disordered serum lipid profile: a Mendelian randomization study

Transcriptomic landscape of endothelial cells: Key tumor microenvironment components indicating variable clinical outcomes in pancreatic ductal adenocarcinoma

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