Metallothionein and cu-chelatin: characterization of metal-binding proteins from tissues of four marine animals

Ridlington, James W.; Chapman, Donald; Goeger, Douglas E.; Whanger, P. D.

doi:10.1016/0305-0491(81)90128-0

Cited by 22 publications

(17 citation statements)

References 16 publications

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“…It appears that very few characterisations of marine mammal metallothioneins have been attempted. The first attempt was realised on sea lion, Zalophus californianus, and kidney and liver of sperm whale, Physeter macrocephalus (Ridlington et al, 1981). Thereafter, MT I and II isolation and characterisation have been performed in the kidney of striped dolphin, Stenella coeruleoalba (Kwohn et al, 1986(Kwohn et al, , 1988.…”

Section: Introductionmentioning

confidence: 99%

White-sided dolphin metallothioneins: purification, characterisation and potential role

Das

Jacob

Bouquegneau

2002

Comparative Biochemistry and Physiology Part C: Toxicology &

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Metallothioneins (MTs) were characterised in the kidneys of a white-sided dolphin Lagenorhynchus acutus stranded along the Belgian coast, displaying high levels of cadmium (Cd) and mercury (Hg) in liver and kidney. The protein has two isoforms: MT-1 and MT-2. MT-1 binds Cu, Zn, Hg and Cd, while MT-2 only binds Zn, Hg and Cd. This suggests different metabolic functions for the two isoforms: MT-1 is mainly involved in Cu homeostasis; MT-2, which was fourfold more abundant than MT-1, detoxifies most of the accumulated cadmium. ᮊ

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Section: Introductionmentioning

confidence: 99%

White-sided dolphin metallothioneins: purification, characterisation and potential role

Das

Jacob

Bouquegneau

2002

Comparative Biochemistry and Physiology Part C: Toxicology &

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“…Many conclusions appear to b e based upon single samples despite the well-established variability that characterizes metal dynamics in aquatic organisms (Luoma & Cain 1979, Strong & Luoma 1981, Lobe1 et al 1982. In fact, where more than 1 cytosol sample from the same species has been analyzed, a wide degree of variability has been observed (Ridlington et al 1981).…”

Section: Introductionmentioning

confidence: 99%

Variability in the fractionation of Cu, Ag, and Zn among cytosolic proteins in the bivalve Macoma balthica

Johansson¹,

Cain²,

Luoma³

1986

Mar. Ecol. Prog. Ser.

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Gel filtration chromatographs of cytosols from the clam Macorna balthica analysed from both field and laboratory treated specimens showed that uptake of Cu, Ag, and Zn in the metallothionein-like protein (MLP) pool follows exposure both in nature and in the laboratory. Specimens collected from San Francisco Bay over 18 mo showed strong temporal variability in the fractionation of the metals among cytosolic proteins. A marked increase in Cu, Ag, and Zn in a very low molecular weight pool occurred when concentrations were highest In the MLP pool. The correlation between total cytosollc metal and MLP-metal also appeared to approach a hyperbolic character at the highest concentrations.

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“…Early studies on MTs in marine mammal were published in the 1970s, and they were in pinnipeds, Callorhinus ursinus -Northern fur seal, Halichoerus grypus -Grey seal - (Olafson and Thompson, 1974), and Zalophus californianus californianus -California sea lion - (Lee et al, 1977); and in 1980 they were also in cetacean, Physeter macrocephalus -Sperm whale - (Ridlington et al, 1981), Monodon monoceros -Narwhal - (Wagemann and Muir, 1984), Stenella coeruleoalba -Striped dolphin - (Kwohn et al, 1986(Kwohn et al, , 1988. The paucity of information about MTs in this biological group is associated with the difficulty to obtain samples in suitable conditions of conservation for this kind of analysis.…”

Section: Discussionmentioning

confidence: 99%

Metallothioneins pattern during ontogeny of coastal dolphin, Pontoporia blainvillei, from Argentina

Polizzi¹,

Romero²,

Boudet³

et al. 2014

Marine Pollution Bulletin

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a b s t r a c tMetallothioneins are signals of metal exposure and widely used in biomonitoring. Franciscana dolphin is an endemic cetacean from the Southwestern Atlantic Ocean, classified as Vulnerable A3d by the IUCN. Metallothionein, copper and zinc in Franciscana were assessed in two geographic groups; one inhabits La Plata River estuary, anthropogenically impacted, and the other inhabits marine coastal ecosystems, with negligible pollution. Despite the environment, hepatic and renal MT concentrations were similar, but there was a declining trend from early to later developmental stages. Metallothionein K/L, Cu and Zn levels corresponded to normal reported ranges. MT was not related with Cd. Fetal concentrations were higher than its mother. These results and the health status of dolphins are suggesting that MT correspond to physiological ranges for the species, and they are closely to homeostasis of Zn and Cu, according to its ontogenetic changes. The information constitutes the first MT information on Franciscana dolphin and can be considered as baseline for the species conservation.

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Metallothionein and cu-chelatin: characterization of metal-binding proteins from tissues of four marine animals

Cited by 22 publications

References 16 publications

White-sided dolphin metallothioneins: purification, characterisation and potential role

White-sided dolphin metallothioneins: purification, characterisation and potential role

Variability in the fractionation of Cu, Ag, and Zn among cytosolic proteins in the bivalve Macoma balthica

Metallothioneins pattern during ontogeny of coastal dolphin, Pontoporia blainvillei, from Argentina

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