Metallothionein-3 is expressed in the brain and various peripheral organs of the rat

Hozumi, Isao; Suzuki, Junko; Kanazawa, Hiroaki; Hara, Akira; Saio, Masanao; Ishii, Takashi; Miyairi, Shinichi; Naganuma, Akira; Tohyama, Chiharu

doi:10.1016/j.neulet.2008.04.047

Cited by 66 publications

(57 citation statements)

References 24 publications

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“…It is immediately brought about a high commitment to the biomedical research and later characterized as the brain specific isomer as well as the potential therapeutic targets for some neurodegenerative diseases therapy (Hozumi et al, 2004). In addition, it has been reported that Cd is involved in the reduction of MT3 mRNA in male and female urogenital tracts and a few other peripheral organs of rats, mice and humans (Hozumi et al, 2008). In 1994, MT4 was identified by chance in human and mouse DNA as a fourth transcriptional active family member.…”

Section: Classification Of Mtmentioning

confidence: 99%

Metallothionein: An Overview on its Metal Homeostatic Regulation in Mammals

Sakulsak

2012

Int. J. Morphol.

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SUMMARY: Metallothionein (MT) is a ubiquitous protein with a low molecular weight of 6-7 kDa weight and it was first identified in the kidney cortex of equines as a cadmium (Cd)-binding protein responsible for the natural accumulation of Cd in the tissue. The mammalian MT contains 61 to 68 amino acid residues, in which 18 to 23 cysteine residues are present. The expression of MT starts by binding of metal transcription factor-1 (MTF-1) to the regulative region of MT gene called metal responsive elements (MREs). The induction of MT through the MREs region can be initiated by several metal ions such as zinc (Zn), copper (Cu) and Cd. However, Zn is the only heavy metal which can reversibly and directly activate the DNA-binding activity of MTF-1. In mammals four types of MT are expressed and they are termed metallothionein-1 (MT1), metallothionein-2 (MT2), metallothionein-3 (MT3), and metallothionein-4 (MT4). MT1 and MT2 are expressed in almost all tissues while MT3 and MT4 are tissue-specific. MT is a key compound involved in the intracellular handling of a variety of essential and nonessential post-transitional metal ions. In order to the heavy metal binding ability of MT, it is suggested to play roles both in the intracellular fixation of essential trace elements Zn and Cu, in controlling the concentrations, and in neutralizing the harmful influences of exposure to toxic elements.

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Section: Classification Of Mtmentioning

confidence: 99%

Metallothionein: An Overview on its Metal Homeostatic Regulation in Mammals

Sakulsak

2012

Int. J. Morphol.

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“…Recently, the epididymis, prostate, uterus, ovary, kidney, intestine and tongue (Moffat and Séguin, 1998;Hozumi et al, 2008). Despite the fact that MT-III is found in various tissues, most studies have focused on understanding the role of MT-III in the brain.…”

Section: Introductionmentioning

confidence: 99%

Resistance of metallothionein-III null mice to cadmium-induced acute hepatotoxicity

Honda

Komuro

Hasegawa³

et al. 2010

J. Toxicol. Sci.

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“…5). Although the presence of liver MT-3 has been controversial (Hozumi et al, 2008;Honda et al, 2010), we found a low concentration of MT-3 mRNA in liver using a modified RT-PCR method. According to the MT-3 genomic PCR product sequence from mouse brain and liver tissue, each tissue exhibited the same MT-3 exon DNA sequence (data not shown).…”

Section: Detection Of Mt-3 Mrna In Mouse Liver and Brainmentioning

confidence: 68%

“…Several antibodies to MT-3 have been reported for immunohistochemical staining (Uchida et al, 1991;Yanagitani et al, 1999;Pang and Ru, 2005), but an ELISA method which is able to determine the MT-3 concentration in clinical and/or experimental specimens has not been reported yet. Therefore, we have developed an ELISA method which may serve as an effective tool for MT-3 research and diagnostic purpose, together with mRNA quantification by RT-PCR (Tsuji et al, 1992;Pang and Ru, 2005;Suzuki et al, 2007;Hozumi et al, 2008;Honda et al, 2010). Most of the MT-3 research has been reported only by mRNA expressions without quantitative protein analysis.…”

Section: Discussionmentioning

confidence: 99%

Determination of metallothionein-3 by a competitive enzyme-linked immunosorbent assay in experimental animals

Saito¹,

Nakazato

Kato³

et al. 2013

J. Toxicol. Sci.

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-An easy and specific enzyme-linked immunoassay (ELISA) for the determination of metallothinein-3 (MT-3) in experimental animals for the research of heavy metal and chemical toxicity has not been reported yet. Therefore, we have developed a competitive ELISA, using a specific monoclonal antibody raised against human recombinant MT-3 (rMT-3). The epitope mapping of the antibody was conducted using mouse, rat, and human MT-3s and peptide fragments of human MT-3. MT-1/2, MT-3 knock-out (KO) mice and human brain and liver were used for the evaluation of the ELISA. A pretreatment method of the tissue homogenates was also examined. The antibody used for the ELISA had the same cross-reactivity with MT-3 in humans and experimental animals. The human MT-3 NH 2 terminal peptide (Fr. 1-17) was the demonstrated epitope of this antibody. The reactivity of this ELISA in brain homogenate of MT-3 KO mouse was significantly low compared with the wild type and MT-1/2 KO mice. The lowest detection limit of the ELISA was 10 ng/ml and over 80% of the spiked rMT-3 was recovered in the brain homogenate. The assay linearity was intact with a 5-fold dilution in the brain homogenate. The inter-and intra-assay CV was 6.5%, respectively. An effective pretreatment procedure of the tissue homogenate was also established for this MT-3 ELISA. In conclusion, this competitive ELISA is an easy and specific method for measuring the brain MT-3 level in experimental animals.

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Metallothionein-3 is expressed in the brain and various peripheral organs of the rat

Cited by 66 publications

References 24 publications

Metallothionein: An Overview on its Metal Homeostatic Regulation in Mammals

Metallothionein: An Overview on its Metal Homeostatic Regulation in Mammals

Resistance of metallothionein-III null mice to cadmium-induced acute hepatotoxicity

Determination of metallothionein-3 by a competitive enzyme-linked immunosorbent assay in experimental animals

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