Metallothionein 1F mRNA Expression Correlates with Histological Grade in Breast Carcinoma

Jin, Rong; Bay, Boon‐Huat; Chow, Vincent T.K.; Tan, Puay‐Hoon

doi:10.1023/a:1010658907462

Cited by 53 publications

(52 citation statements)

References 21 publications

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“…Although metatypic BCC is regarded as an aggressive subtype of BCC, it manifested the lowest MT-I/II expression of the analyzed subtypes. We used IHC techniques to determine MT-I/II expression, but recent studies have shown that only particular MT-I/II isoforms may contribute to cancer cell aggressiveness in some tumor types [31][32][33][34][35]. In our study, we noted the highest Ki-67 antigen expression in this subtype.…”

Section: Discussionmentioning

confidence: 57%

Expression of metallothionein I/II and Ki-67 antigen in various histological types of basal cell carcinoma

Bieniek¹,

Puła²,

Piotrowska³

et al. 2012

Folia Histochem Cytobiol.

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Basal cell carcinoma (BCC) is the most frequent skin cancer, with many different histological subtypes. Recent studies have investigated the expression of proliferative markers, but little is known about the expression of metallothioneins (MT) in different histological subtypes of this cancer and their impact on proliferation intensity in BCC. In this study, we examined MT-I/II expression by immunohistochemistry in 58 different histological subtypes of BCC (38 nodular, six adenoid, eight infiltrative, and six metatypic cases) and correlated its expression with tumor size and Ki-67 proliferation rate. Statistical analysis revealed no significant differences in the expression of studied markers in regard to the histological subtype. A positive correlation between MT and Ki-67 expression was observed for all the studied cases (r = 0.26; p = 0.049), but was even stronger in the metatypic subtype of BCC (r = 0.85; p = 0.033). MT and Ki-67 expression did not correlate with tumor size. In conclusion, it seems that metallothioneins may have an impact on the proliferation rate of BCC, but further studies are required to determine whether MT may be a risk factor of recurrences.

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Section: Discussionmentioning

confidence: 57%

Expression of metallothionein I/II and Ki-67 antigen in various histological types of basal cell carcinoma

Bieniek¹,

Puła²,

Piotrowska³

et al. 2012

Folia Histochem Cytobiol.

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“…MT overexpression in ductal breast cancer [38] appears to be predominantly associated with more aggressive and highergrade tumors. Human MT proteins encoded by a family of genes consisting of 10 functional MT isoforms may play different roles during development or under various physiological conditions [35,38]. Specific roles played by metallothioneins 1G and 1L, if any, as is related to invasiveness are not known.…”

Section: Differential Expressions Of Genes In Bt-549 and Mda-mb-468 Cmentioning

confidence: 97%

“…Metallothioneins (MTs), a group of inducible, low molecular weight, cysteine-rich proteins [35], are known to influence tumor growth by affecting both cell proliferation and death [36,37]. MT overexpression in ductal breast cancer [38] appears to be predominantly associated with more aggressive and highergrade tumors. Human MT proteins encoded by a family of genes consisting of 10 functional MT isoforms may play different roles during development or under various physiological conditions [35,38].…”

Section: Differential Expressions Of Genes In Bt-549 and Mda-mb-468 Cmentioning

confidence: 99%

Negative regulation of the expressions of cytokeratins 8 and 19 by SLUG repressor protein in human breast cells

Tripathi

Misra

Chaudhuri

2005

Biochemical and Biophysical Research Communications

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Invasiveness of tumor cells is often determined by the profile of their expressed genes. To determine the gene expression differences between an invasive and a non-invasive human breast tumor cells, we selected BT-549 (invasive) and MDA-MB-468 (non-invasive) cells, and compared their transcriptomes by cDNA microarray analysis. Among the significant differences in gene expressions, notable are the up-regulation of cytokeratins 8 and 19, and down-regulation of metallothioneins 1G and IL in MDA-MB-468 cells. Since MDA-MB-468 cells do not express SLUG, a member of a small family of E2-box-binding zinc finger silencer proteins, we studied whether the cytokeratin gene overexpressions in these cells are due to the absence of SLUG. Inducible expression of SLUG in MDA-MB-468 cells inhibited the expressions of the cytokeratin 8 and 19 but not others as was revealed by microarray analysis. Similarly, siRNA knock down of SLUG in BT-549 cells increased the expressions of those cytokeratin mRNAs. SLUG levels in the cell regulated the function of cytokeratins 8 and 19 gene promoters. We conclude that the expressions of cytokeratins and metallothioneins may be associated with the differential invasive behaviors of these breast tumor cells and SLUG may have regulatory roles in this process. KeywordsInvasiveness; Breast tumor; SLUG; Cytokeratin 8; Cytokeratin 19; Metallothionein 1G; Metallothionein 1L; siRNA; Microarray A serious gap in our understanding of cancer concerns malignancy and metastasis. We have yet to clearly identify gene defects that specifically permit cells to invade surrounding tissues, spread through the body, and form metastases. Local invasiveness requires a break down of the mechanisms that normally hold epithelial cells together. To determine the gene expression differences between an invasive and a non-invasive human breast tumor cells, we selected BT-549 (invasive) and MDA-MB-468 (non-invasive) cells, and compared their transcriptomes by cDNA microarray analysis. One of the known genotypic differences between these two cells is that BT-549 expresses the zinc finger repressor protein SLUG but the MDA-MB-468 cells do not [1]. Hence, we tested whether any gene overexpressed in the MDA-MB-468 cells is regulated by SLUG.We paid particular attention to SLUG because it is implicated as one of the determinants of tumor cell invasiveness [2][3][4][5][6]. This protein is a member of a superfamily of transcriptional regulators that are implicated in the formation of mesoderm and neural crest, as well as in We then overexpressed SLUG in MDA-MB-468 cells from a doxycycline-inducible promoter and compared the gene expression profile in the presence or absence of the inducer. We identified that cytokeratins 8 and 19 genes are repressed when SLUG expression is induced. We have verified the role of SLUG in regulating these cytokeratins by siRNA-mediated knock down of SLUG in BT-549 cells. We also report here our preliminary characterization of the promoters of these cytokeratin genes as it is regulated by SLUG exp...

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“…In human, genes encoding for MTs are located on chromosome 16, within the 16q13 region (Mididoddi et al, 1996). MTs are intracellular proteins and their presence was demonstrated in the cytoplasm and in the cell nucleus (Bay et al, 2001). Their synthesis can be induced by several substances, such as heavy metals, hormones, cytokines, growth factors, organic compounds and free radicals (Samson et al, 1998;Haq et al, 2003;Ghoshal et al, 2001).…”

Section: Mt Structure and Synthesismentioning

confidence: 99%

“…Investigators have focused on the significance of MT in the process of carcinogenesis and tumour progression but also on the involvement of the proteins in development of tumour chemoresistance as well as the possible role of MT expression as a prognostic and predictive factor. Results of several investigations suggest that the role of MT expression in tumour cells may be linked to the processes of proliferation and apoptosis Bay et al, 2001;Shimoda et al, 2003). MT functions as a donor of zinc ions for transcription factors and enzymes involved in the processes of DNA and protein synthesis.…”

Section: Role Of Mt In a Neoplastic Processmentioning

confidence: 99%

Expression of Metallothionein in Oral Cancer

Dzięgiel¹,

Puła²,

Podhorska-Okołów³

2012

Oral Cancer

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Metallothionein 1F mRNA Expression Correlates with Histological Grade in Breast Carcinoma

Cited by 53 publications

References 21 publications

Expression of metallothionein I/II and Ki-67 antigen in various histological types of basal cell carcinoma

Expression of metallothionein I/II and Ki-67 antigen in various histological types of basal cell carcinoma

Negative regulation of the expressions of cytokeratins 8 and 19 by SLUG repressor protein in human breast cells

Expression of Metallothionein in Oral Cancer

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