Metal speciation analysis in eel (Anguilla anguilla) metallothioneins by anionic exchange-FPLC-isotope dilution-ICP-MS

Rodríguez-Cea, Andrés; Campa, María del Rosario Fernández de la; González, Elisa Blanco; Fernández, Balbina Andón; Sanz‐Medel, Alfredo

doi:10.1039/b306746g

Cited by 40 publications

(25 citation statements)

References 39 publications

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“…Based on the advantages of ICP-TOF-MS, the combination of species-unspecific isotope dilution methods on-line with liquid chromatography (LC)-ICP-TOF-MS has been exploited for the quantitative determination of Cu, Zn, and Cd associated with the MT fraction in fish cytosolic extracts [52]. Recently, fast protein LC (FPLC) in combination with IDICP-QMS was applied to the metal fractionation of MT isoforms in hepatic cytosols of eels [45,53]. Nevertheless, the lack of standards for the different metal-binding proteins present makes on-line quantification of metal species a difficult task.…”

Section: Chromatographic and Electrophoretic Methodsmentioning

confidence: 99%

Review on Methods for Determination of Metallothioneins in Aquatic Organisms

Shariati

2010

Biol Trace Elem Res

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One aspect of environmental degradation in coastal areas is pollution from toxic metals, which are persistent and are bioaccumulated by marine organisms, with serious public health implications. A conventional monitoring system of environmental metal pollution includes measuring the level of selected metals in the whole organism or in respective organs. However, measuring only the metal content in particular organs does not give information about its effect at the subcellular level. Therefore, the evaluation of biochemical biomarker metallothionein may be useful in assessing metal exposure and the prediction of potential detrimental effects induced by metal contamination. There are some methods for the determination of metallothioneins including spectrophotometric method, electrochemical methods, chromatography, saturation-based methods, immunological methods, electrophoresis, and RT-PCR. In this paper, different methods are discussed briefly and the comparison between them will be presented.

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Section: Chromatographic and Electrophoretic Methodsmentioning

confidence: 99%

Review on Methods for Determination of Metallothioneins in Aquatic Organisms

Shariati

2010

Biol Trace Elem Res

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“…The chemical composition of breast milk changes with time after the delivery from colostrum (days 1-3) to mature milk (days [14][15][16][17][18][19][20][21][22][23][24][25][26][27][28] [4]. Milk is a basic food in humans and the primary source of nutrition of the newborn during the first months of life.…”

Section: Milk As Source Of Trace Elementsmentioning

confidence: 99%

“…The assumption that only one and pure isotope is added to the biological system is not correct and is therefore necessary to take this into due account in the mathematical calculations [26]. The assumption that only one and pure isotope is added to the biological system is not correct and is therefore necessary to take this into due account in the mathematical calculations [26].…”

Section: Stable Isotopes and Trace Elements Metabolismmentioning

confidence: 99%

A New Tool Based on the Use of Stable Isotopes and Isotope Pattern Deconvolution (IPD)‐ICP‐MS for Nutritional and Clinical Studies

Iglesias

Fernández‐Sánchez

Sanz‐Medel

2012

Analytical Techniques for Clinical Chemistry

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“…Species-unspecific isotope dilution for HPLC coupling has been described for the determination of Cd, Cu and Zn in metallothioneins extracted from eel liver, 157 while Fe, Cu and Zn were determined in proteins separated from human serum. 158 This technique has also been applied to iodine determination in humic substances, 159 as well as selenium speciation in human serum.…”

Section: Isotope Dilution In Speciation With Hplc-icp-msmentioning

confidence: 99%