Metal organic frameworks as a compatible reinforcement in a biopolymer bone scaffold

Yang, Youwen; Zan, Jun; Yang, Wenjing; Qi, Fangwei; He, Chongxian; Huang, Shuhui; Peng, Shuping; Shuai, Cijun

doi:10.1039/c9qm00772e

Cited by 70 publications

(48 citation statements)

References 54 publications

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“…Using stem cell self‐renewal and differentiation ability to treat osteoporosis may be the most potential way of bone repair, which is expected to solve the medical problems of bone regeneration and repair 29 . In recent years, biological scaffolds have made rapid progress 30‐33 . It is particularly urgent to clarify the mechanism of stem cell differentiation and develop new strategies to induce stem cell differentiation.…”

Section: Discussionmentioning

confidence: 99%

Linc02349 promotes osteogenesis of human umbilical cord‐derived stem cells by acting as a competing endogenous RNA for miR‐25‐3p and miR‐33b‐5p

et al. 2020

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Objectives: Increasing evidences suggest that inducing mesenchymal stem cells to differentiate into osteoblasts has been as an especially important component in the prevention and therapy for degenerative bone disease. Here, we identify a novel lncRNA, linc02349, which increases significantly during osteogenic differentiation. Materials and methods:Human umbilical cord-derived stem cells (hUC-MSCs) and dental pulp mesenchymal stem cells were used. Overexpression and knockdown of linc02349 in cell lines were generated using lentiviral-mediated gene delivery method.Bioinformatics prediction, Ago2-RIP assay and dual-luciferase reporter system were employed to examine miRNA which interacts with linc02349. The RNA FISH assay was performed to identify the subcelluar location of linc02349. Alizarin Red S staining, ALP staining and qPCR were applied to identify the osteogenic differentiation. The potential linc02349-regulated genes, miR-25-3p and miR-33b-5p, were explored by ChIP, RIP and Western blotting assays. Micro-CT was used to measure the osteogenic content in bone formation assay in vivo. Results: Linc02349 overexpression improves osteogenic differentiation by in vitroand in vivo analysis. Mechanistically, linc02349 acts as a molecular sponge for miR-25-3p and miR-33b-5p to control expression abundance of SMAD5 and Wnt10b,

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Section: Discussionmentioning

confidence: 99%

Linc02349 promotes osteogenesis of human umbilical cord‐derived stem cells by acting as a competing endogenous RNA for miR‐25‐3p and miR‐33b‐5p

et al. 2020

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“…Based on the results of previous studies ( 2 , 30 – 32 ), combining bioactive molecules, such as growth factors, peptides and small molecules with corresponding bone scaffold may lead to effective clinical transformation and provide new insights into the future of bone transplantation. Notably, this study demonstrated that NPY signaling directly promotes osteogenic differentiation of MC3T3-E1 cells by upregulating Runx2 and osterix in vitro .…”

Section: Discussionmentioning

confidence: 99%

Neuropeptide�Y upregulates Runx2 and osterix and enhances osteogenesis in mouse MC3T3‑E1 cells via an autocrine mechanism

Zhang

Zhou

et al. 2020

Mol Med Rep

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The neuropeptide Y (nPY) system is considered one of the primary neural signaling pathways. nPY, produced by osteoblasts and other peripheral tissues, is known to inhibit biological functions of osteoblasts. However, until recently, little was known of the autocrine mechanism by which nPY is regulated. To investigate this mechanism, overexpression plasmids and small interfering rna (sirna) targeting nPY were transfected into the Mc3T3-e1 cell line to observe its effects on osteogenesis. nPY overexpression was found to markedly enhance the osteogenic ability of Mc3T3-e1 cells by an autocrine mechanism, coincident with the upregulation of osterix and runt-related transcription factor 2 (runx2). Furthermore, nPY increased the activities of alkaline phosphatase (alP) and osteocalcin (ocn) by upregulating their osteoblastic expression in vitro (as well as that of osterix and runx2). Following transfection with nPY-sirna, the osteoblastic ability of MC3T3-E1 cells was markedly decreased, and NPY deficiency inhibited the protein expression of osterix, runx2, ocn and alP in primary osteoblasts. collectively, these results indicated that nPY played an important role in osteoblast differentiation by regulating the osterix and runx2 pathways.

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“…Various techniques such as salt leaching 6 , fibrous fabric processing 7 , woven fabric processing 8 , gas forming 9 , emulsion freeze-drying 10 , electro spinning 11 , three dimensional printing 12 , and phase separation 13 have been developed to fabricate porous biodegradable polymer scaffolds. In addition, various techniques for the fabrication of biodegradable polymeric scaffolds have been tried recently to increase the strength and the antibacterial activity of scaffolds 14 – 16 .…”

Section: Introductionmentioning

confidence: 99%

Formation of oriented fishbone-like pores in biodegradable polymer scaffolds using directional phase-separation processing

2020

Sci Rep

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The scaffold is a dreamed biomaterial of tissue engineers which can culture cells three-dimensionally outgrowing the two-dimensional cell culture in a petri dish to repair or regenerate tissues and organs. To maximize the performance of this dreamed material, complex three-dimensional (3D) structures should be generated with a simple technique and nontoxic ingredients. Many tissues have tubular or fibrous bundle architectures such as nerve, muscle, tendon, ligament, blood vessel, bone and teeth. The concept of mimicking the extracellualr matrix in real tissue has recently been applied to scaffold development. In this study, a novel method for preparing the poly(l-lactic acid) (PLLA) scaffold with a tubular architecture is presented. Solid-liquid phase-separation was applied to form tubular pores in the scaffold using the directional freezing apparatus. Pores formed in this manner exhibited a fishbone like morphology due to the two crystalline phases of 1,4-dioxane. A tubular diameter of ca. 60-250 μm was achieved by regulating the PLLA concentration and the cooling rate. The compressive modulus of the fishbone-like porous scaffold showed higher values than that of non-directional porous scaffold. Scaffolds are biomaterials designed by tissue engineers to support three-dimensional tissue formation to facilitate repair and regeneration of damaged tissues and organs. In these scaffolds, biodegradable polymer scaffolds are particularly prevalent because over time the artificial scaffold and be completely replaced by the patient's own cellular material. Currently, the design and fabrication of synthetic biodegradable scaffolds is driven by two materials categories: (1) biodegradable and bioresorbable polymers, which have been effectively used for clinically established products, including polyglic acid (PGA) 1 , poly (l-lactic acid) (PLLA) 2 , poly (d,l-lactic acid) (PDLLA) 3 , and polycaprolactone (PCL) 4 ; (2) novel di-and tri-block copolymers which predominantly incorporated PGA, PLA, and PCL in different chain arrangements which confer both degradation and mechanical property customization 5. Various techniques such as salt leaching 6 , fibrous fabric processing 7 , woven fabric processing 8 , gas forming 9 , emulsion freeze-drying 10 , electro spinning 11 , three dimensional printing 12 , and phase separation 13 have been developed to fabricate porous biodegradable polymer scaffolds. In addition, various techniques for the fabrication of biodegradable polymeric scaffolds have been tried recently to increase the strength and the antibacterial activity of scaffolds 14-16. The concept of mimicking the extracellualr matrix (ECM) in real tissue has been applied to develop scaffolds at macro-and nano-levels. In this study, the microtubular PLLA scaffolds were fabricated to mimic the macrolevel morphology of the ECM because many organs and tissues have tubular or fibrous bundle architectures such as nerve, muscle, tendon, ligament, blood vessel, bone and teeth. Solid-liquid phase separation was used to fabricate tubular...

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Metal organic frameworks as a compatible reinforcement in a biopolymer bone scaffold

Abstract: The insufficient mechanical strength and slow degradation rate of poly-l-lactic acid (PLLA) limit its applications in bone repair.

Cited by 70 publications

References 54 publications

Linc02349 promotes osteogenesis of human umbilical cord‐derived stem cells by acting as a competing endogenous RNA for miR‐25‐3p and miR‐33b‐5p

Linc02349 promotes osteogenesis of human umbilical cord‐derived stem cells by acting as a competing endogenous RNA for miR‐25‐3p and miR‐33b‐5p

Neuropeptide�Y upregulates Runx2 and osterix and enhances osteogenesis in mouse MC3T3‑E1 cells via an autocrine mechanism

Formation of oriented fishbone-like pores in biodegradable polymer scaffolds using directional phase-separation processing

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