Metal Organic Frame-Upconverting Nanoparticle Assemblies for the FRET Based Sensor Detection of Bisphenol A in High-Salt Foods

Abstract

To resolve the occurrence of unfulfillable detection in high-salts foods, we used fluorescence resonant energy transfer (FRET) sensors based on nanoparticle upconversion. In this study, we developed a novel FRET sensor for the detection of bisphenol A (BPA) in high-salt foods. We based this approach on the assembly of aptamer modified upconversion nanoparticles (DNA1-UCNPs) and complementary DNA modified metal organic frames (DNA2-MOFs), which possessed corresponding wavelength absorption. Targeting BPA signal transduction was performed using the BPA aptamer, via competitive recognition between the BPA analyte and complementary DNA sequences in a high-salt solution. Sensor adaption in high-salt samples was attributed to functional hydrophilic groups, modified in the MOFs, and the enhanced colloidal stability of these MOFs. The MOF-UCNP assembly displayed considerable analytical performance in terms of BPA detection, with a linear range of 0.1–100 nM, and a limit of detection (LOD) of 0.02 nM, in a 340 mM NaCl food sample (the energy drink, Gatorade). Thus, this method provides a solid basis for small molecules detection in high-salt foods.

Synthesis of Metal–Organic Frameworks Quantum Dots Composites as Sensors for Endocrine-Disrupting Chemicals

“…Xui et al [ 163 ] used a florescence resonance energy transfer (FRET) sensor centered on nanomaterials up-conversion to determine the presence of pollutants in salty foods. This FRET technique was developed to detect bisphenol A, a common pollutant/contaminant in high-salt foods.…”

Heterostructures Made of Upconversion Nanoparticles and Metal–Organic Frameworks for Biomedical Applications

“…[ 52 , 53 , 54 ] Cheng and co‐workers synthesized UCNP‐MOF structures using aptamer modified UCNPs and MOFs, which could be used for the detection of bisphenol A in high‐salt foods. [ 55 ] Aside from DNA base pairing interactions, π – π stacking interactions were adopted to adsorb aptamer‐modified UCNPs onto the surface of MIL‐101 to construct nanosensors for specific response to T‐2 toxin. [ 41 ]…”

Functional Hybrid Micro/Nanoentities Promote Agro-Food Safety Inspection

“…The results showed a linear range of 0.1–100 nM and a detection limit of 0.02 nM. Therefore, this method is more feasible in actual high-salt food detection (Figure ).…”

“…MOF–UCNP platform assembly for the detection of BPA in a high-salt solution. This figure was reprinted with permission from ref . Copyright 2020 Frontiers.…”